Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.

Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.

The bacterium Burkholderia pseudomallei causes melioidosis, a rare but serious illness that can be fatal if untreated or misdiagnosed. Species-specific PCR assays provide a technically simple method for differentiating B. pseudomallei from near-neighbor species. However, substantial genetic diversit...

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Main Authors: Erin P Price, Julia L Dale, James M Cook, Derek S Sarovich, Meagan L Seymour, Jennifer L Ginther, Emily L Kaufman, Stephen M Beckstrom-Sternberg, Mark Mayo, Mirjam Kaestli, Mindy B Glass, Jay E Gee, Vanaporn Wuthiekanun, Jeffrey M Warner, Anthony Baker, Jeffrey T Foster, Patrick Tan, Apichai Tuanyok, Direk Limmathurotsakul, Sharon J Peacock, Bart J Currie, David M Wagner, Paul Keim, Talima Pearson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0037723&type=printable
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0037723&type=printable

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