Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep
Fatty acid contributes notably to meat nutrition value. A previous study investigated how complex plant extracts (CPE) can improve the fatty acid composition of ruminants, but the molecular mechanism remains unknown. This study aimed to examine the effect of dietary CPE supplementation on sheep grow...
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2025-06-01
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| author | Hui Guo Ruixue Nie Tao Guo Chang Gao Jinju Mao Yuchao Hu Wenwen Wang Na Liu Xiaoping An Jingwei Qi Yuan Wang |
| author_facet | Hui Guo Ruixue Nie Tao Guo Chang Gao Jinju Mao Yuchao Hu Wenwen Wang Na Liu Xiaoping An Jingwei Qi Yuan Wang |
| author_sort | Hui Guo |
| collection | DOAJ |
| description | Fatty acid contributes notably to meat nutrition value. A previous study investigated how complex plant extracts (CPE) can improve the fatty acid composition of ruminants, but the molecular mechanism remains unknown. This study aimed to examine the effect of dietary CPE supplementation on sheep growth performance and fatty acid composition in back fat (BF), combining lipidomics and transcriptomics analyses to explain the underlying mechanisms. Thirty-six female sheep, weighing 29.92 ± 2.52 kg and of a similar age (~4 months old), were randomly assigned into two groups: one received a basal diet (CTRL group), and the other received the same diet supplemented with 80 mg/kg of CPE (CPE group) for 75 days. The results revealed that the values of carcass fat content (GR) in the CPE group were significantly increased (<i>p</i> = 0.008), and the composition of fatty acid was changed. Lipidomic analysis indicated that CPE modulated lipid metabolism by regulating the contents of lipid molecules such as phosphatidylcholine (PC), fatty acyls (FAs), cardiolipin (CL), and triglyceride (TAG). After the addition of CPE, the lipid metabolism of BF was regulated mainly by regulating the glycerophospholipid metabolism, TNF signaling pathway, and cytokine–cytokine receptor interaction signaling pathway. These results revealed that changes in fatty acids were affected by the added CPE and corresponding molecular changes, which may provide new insights on the molecular level for applying CPE in sheep to improve fatty acid composition. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-0c10a5a13bd34bc5887d677985e611552025-08-20T02:32:54ZengMDPI AGAnimals2076-26152025-06-011511164510.3390/ani15111645Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in SheepHui Guo0Ruixue Nie1Tao Guo2Chang Gao3Jinju Mao4Yuchao Hu5Wenwen Wang6Na Liu7Xiaoping An8Jingwei Qi9Yuan Wang10College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaFatty acid contributes notably to meat nutrition value. A previous study investigated how complex plant extracts (CPE) can improve the fatty acid composition of ruminants, but the molecular mechanism remains unknown. This study aimed to examine the effect of dietary CPE supplementation on sheep growth performance and fatty acid composition in back fat (BF), combining lipidomics and transcriptomics analyses to explain the underlying mechanisms. Thirty-six female sheep, weighing 29.92 ± 2.52 kg and of a similar age (~4 months old), were randomly assigned into two groups: one received a basal diet (CTRL group), and the other received the same diet supplemented with 80 mg/kg of CPE (CPE group) for 75 days. The results revealed that the values of carcass fat content (GR) in the CPE group were significantly increased (<i>p</i> = 0.008), and the composition of fatty acid was changed. Lipidomic analysis indicated that CPE modulated lipid metabolism by regulating the contents of lipid molecules such as phosphatidylcholine (PC), fatty acyls (FAs), cardiolipin (CL), and triglyceride (TAG). After the addition of CPE, the lipid metabolism of BF was regulated mainly by regulating the glycerophospholipid metabolism, TNF signaling pathway, and cytokine–cytokine receptor interaction signaling pathway. These results revealed that changes in fatty acids were affected by the added CPE and corresponding molecular changes, which may provide new insights on the molecular level for applying CPE in sheep to improve fatty acid composition.https://www.mdpi.com/2076-2615/15/11/1645complex plant extractmolecular mechanismsgene expression regulationlipid metabolismmulti-omics |
| spellingShingle | Hui Guo Ruixue Nie Tao Guo Chang Gao Jinju Mao Yuchao Hu Wenwen Wang Na Liu Xiaoping An Jingwei Qi Yuan Wang Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep Animals complex plant extract molecular mechanisms gene expression regulation lipid metabolism multi-omics |
| title | Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep |
| title_full | Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep |
| title_fullStr | Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep |
| title_full_unstemmed | Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep |
| title_short | Lipidomics and Transcriptomics Analyses Reveal Dietary Complex Plant Extracts Improve Lipid Composition of Back Fat in Sheep |
| title_sort | lipidomics and transcriptomics analyses reveal dietary complex plant extracts improve lipid composition of back fat in sheep |
| topic | complex plant extract molecular mechanisms gene expression regulation lipid metabolism multi-omics |
| url | https://www.mdpi.com/2076-2615/15/11/1645 |
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