JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis
Abstract New hematopoietic cell models have recently emerged through immortalization of CD34 cells to study and understand various molecular mechanisms of erythropoiesis. Here, we characterize the JK-1 CML-derived cell line, previously shown to spontaneously differentiate without cytokines. Using an...
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2024-10-01
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| author | Sylvain Metral Sandrine Genetet Benoît Gamain Isabelle Mouro-Chanteloup |
| author_facet | Sylvain Metral Sandrine Genetet Benoît Gamain Isabelle Mouro-Chanteloup |
| author_sort | Sylvain Metral |
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| description | Abstract New hematopoietic cell models have recently emerged through immortalization of CD34 cells to study and understand various molecular mechanisms of erythropoiesis. Here, we characterize the JK-1 CML-derived cell line, previously shown to spontaneously differentiate without cytokines. Using an epigenetic differentiation inhibitor that keeps JK-1 in an early differentiation phase, we characterized 2 progenitor stages: BFU-E JK-1 and CFU-E JK-1 with CD34+/CD36− and CD34−/CD36 + phenotypes respectively. Then, using the PFI-1 inducer known to synchronously control the terminal differentiation of JK-1 cells, 5 precursor stages were obtained (ProE, Baso1-2, PolyC, OrthoC) and characterized via cell morphology, CD49a and Band3 markers. Enlarged phenotyping was carried out for the earlier phase, and expression kinetics of membrane proteins such as RhAG, RhD/CE, CD47, DARC and CD44 were performed on each stage of the terminal phase. Furthermore, since JK-1 offers the unique property of covering a broad spectrum of differentiation stages, we explored deeper the GATA2/GATA1 and the non-erythroid/erythroid spectrin ‘switching’. The possibility of obtaining large quantities of JK-1 cells at each stage of differentiation, as shown in this study, as well as the potential to genetically modify these cells, via CrisprCas9, makes their use of considerable interest for studying pathologies occurring during erythropoiesis. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-0bdf975c154947b18140547ea6c60d392025-01-12T12:24:54ZengNature PortfolioScientific Reports2045-23222024-10-0114111110.1038/s41598-024-76897-7JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesisSylvain Metral0Sandrine Genetet1Benoît Gamain2Isabelle Mouro-Chanteloup3Université Paris Cité and Université des Antilles, Inserm U1134, BIGRUniversité Paris Cité and Université des Antilles, Inserm U1134, BIGRSorbonne Université, Inserm U1135, CNRS ERL 8255Sorbonne Université, Inserm U1135, CNRS ERL 8255Abstract New hematopoietic cell models have recently emerged through immortalization of CD34 cells to study and understand various molecular mechanisms of erythropoiesis. Here, we characterize the JK-1 CML-derived cell line, previously shown to spontaneously differentiate without cytokines. Using an epigenetic differentiation inhibitor that keeps JK-1 in an early differentiation phase, we characterized 2 progenitor stages: BFU-E JK-1 and CFU-E JK-1 with CD34+/CD36− and CD34−/CD36 + phenotypes respectively. Then, using the PFI-1 inducer known to synchronously control the terminal differentiation of JK-1 cells, 5 precursor stages were obtained (ProE, Baso1-2, PolyC, OrthoC) and characterized via cell morphology, CD49a and Band3 markers. Enlarged phenotyping was carried out for the earlier phase, and expression kinetics of membrane proteins such as RhAG, RhD/CE, CD47, DARC and CD44 were performed on each stage of the terminal phase. Furthermore, since JK-1 offers the unique property of covering a broad spectrum of differentiation stages, we explored deeper the GATA2/GATA1 and the non-erythroid/erythroid spectrin ‘switching’. The possibility of obtaining large quantities of JK-1 cells at each stage of differentiation, as shown in this study, as well as the potential to genetically modify these cells, via CrisprCas9, makes their use of considerable interest for studying pathologies occurring during erythropoiesis.https://doi.org/10.1038/s41598-024-76897-7ErythropoiesisProgenitorPrecursorJK-1 cellsExpression switching |
| spellingShingle | Sylvain Metral Sandrine Genetet Benoît Gamain Isabelle Mouro-Chanteloup JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis Scientific Reports Erythropoiesis Progenitor Precursor JK-1 cells Expression switching |
| title | JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| title_full | JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| title_fullStr | JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| title_full_unstemmed | JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| title_short | JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| title_sort | jk 1 a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis |
| topic | Erythropoiesis Progenitor Precursor JK-1 cells Expression switching |
| url | https://doi.org/10.1038/s41598-024-76897-7 |
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