Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway
In triple-negative breast cancer, intratumoral hypoxia drives tumor progression via HIF-1α-mediated angiogenesis. The study aims to analyze the efficacy of berberine chloride on tumor proliferation and angiogenesis in triple-negative breast cancer mediated by HIF-1α. The triple-negative breast canc...
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Bangladesh Pharmacological Society
2025-05-01
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| Series: | Bangladesh Journal of Pharmacology |
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| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/80837 |
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| author | Nishmaa Sulthan Mohideen Venkatalakshmi Ranganathan Fazeela Mahaboob Begum S. Mohamed Nainar |
| author_facet | Nishmaa Sulthan Mohideen Venkatalakshmi Ranganathan Fazeela Mahaboob Begum S. Mohamed Nainar |
| author_sort | Nishmaa Sulthan Mohideen |
| collection | DOAJ |
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In triple-negative breast cancer, intratumoral hypoxia drives tumor progression via HIF-1α-mediated angiogenesis. The study aims to analyze the efficacy of berberine chloride on tumor proliferation and angiogenesis in triple-negative breast cancer mediated by HIF-1α. The triple-negative breast cancer cells were subjected to berberine chloride, and the effects on cell viability, apoptosis, and angiogenic gene expression were assessed. MTT assay determined the IC50 as 9.5 µM. Fluorescent imaging confirmed apoptosis through membrane disintegration, fragmented DNA, and the presence of apoptotic bodies. Real-time PCR analysis showed significant down-regulation of HIF-1α (p<0.01), VEGF (p<0.05), Ang1 (p<0.05), and Ang2 (p<0.01) after berberine chloride treatment. These findings indicate the efficacy of berberine chloride in inhibiting triple-negative breast cancer cell proliferation and angiogenesis, highlighting its potential as a therapeutic candidate.
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| format | Article |
| id | doaj-art-0b31fabb0b084ae8b0ad3407d52d52e7 |
| institution | OA Journals |
| issn | 1991-0088 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj-art-0b31fabb0b084ae8b0ad3407d52d52e72025-08-20T02:29:24ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882025-05-0120210.3329/bjp.v20i2.80837Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathwayNishmaa Sulthan Mohideen0Venkatalakshmi Ranganathan1Fazeela Mahaboob Begum S. Mohamed Nainar2School of Life Sciences, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai-600048, India.Department of Pharmaceutics, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai-600048, India.School of Life Sciences, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai-600048, India. In triple-negative breast cancer, intratumoral hypoxia drives tumor progression via HIF-1α-mediated angiogenesis. The study aims to analyze the efficacy of berberine chloride on tumor proliferation and angiogenesis in triple-negative breast cancer mediated by HIF-1α. The triple-negative breast cancer cells were subjected to berberine chloride, and the effects on cell viability, apoptosis, and angiogenic gene expression were assessed. MTT assay determined the IC50 as 9.5 µM. Fluorescent imaging confirmed apoptosis through membrane disintegration, fragmented DNA, and the presence of apoptotic bodies. Real-time PCR analysis showed significant down-regulation of HIF-1α (p<0.01), VEGF (p<0.05), Ang1 (p<0.05), and Ang2 (p<0.01) after berberine chloride treatment. These findings indicate the efficacy of berberine chloride in inhibiting triple-negative breast cancer cell proliferation and angiogenesis, highlighting its potential as a therapeutic candidate. https://www.banglajol.info/index.php/BJP/article/view/80837Ang pathwayAngiogenesisBerberine chlorideHIF-1α pathwayMTT assayTriple-negative breast cancer |
| spellingShingle | Nishmaa Sulthan Mohideen Venkatalakshmi Ranganathan Fazeela Mahaboob Begum S. Mohamed Nainar Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway Bangladesh Journal of Pharmacology Ang pathway Angiogenesis Berberine chloride HIF-1α pathway MTT assay Triple-negative breast cancer |
| title | Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway |
| title_full | Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway |
| title_fullStr | Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway |
| title_full_unstemmed | Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway |
| title_short | Berberine chloride inhibits proliferation and angiogenesis in triple-negative breast cancer by down-regulating HIF-1α/VEGF/Ang pathway |
| title_sort | berberine chloride inhibits proliferation and angiogenesis in triple negative breast cancer by down regulating hif 1α vegf ang pathway |
| topic | Ang pathway Angiogenesis Berberine chloride HIF-1α pathway MTT assay Triple-negative breast cancer |
| url | https://www.banglajol.info/index.php/BJP/article/view/80837 |
| work_keys_str_mv | AT nishmaasulthanmohideen berberinechlorideinhibitsproliferationandangiogenesisintriplenegativebreastcancerbydownregulatinghif1avegfangpathway AT venkatalakshmiranganathan berberinechlorideinhibitsproliferationandangiogenesisintriplenegativebreastcancerbydownregulatinghif1avegfangpathway AT fazeelamahaboobbegumsmohamednainar berberinechlorideinhibitsproliferationandangiogenesisintriplenegativebreastcancerbydownregulatinghif1avegfangpathway |