Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)
The mycelial biomass, antioxidant activity and production of extra- and intra-cellular polysaccharides production [EPS, IPS] of Cordyceps militaris strains AG-1, PSJ-1 were evaluated under different submerged liquid culture (SLC) conditions. At 24 0C mycelial biomass and polysaccharide production of...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2020-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12966 |
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| author | Dang Ngoc HUNG Li Chun WANG Liang Horng LAY Vu Thi PHUONG |
| author_facet | Dang Ngoc HUNG Li Chun WANG Liang Horng LAY Vu Thi PHUONG |
| author_sort | Dang Ngoc HUNG |
| collection | DOAJ |
| description | The mycelial biomass, antioxidant activity and production of extra- and intra-cellular polysaccharides production [EPS, IPS] of Cordyceps militaris strains AG-1, PSJ-1 were evaluated under different submerged liquid culture (SLC) conditions. At 24 0C mycelial biomass and polysaccharide production of AG-1, PSJ-1 was optimal using PVC media and static culture conditions; (AG-1: 21.85 ± 1.00; PSJ-1: 18.20 ± 1.84 g l-1 ), and oven drying at 40 oC (AG-1: 25.95 ± 0.84, PSJ-1: 23.55 ± 0.69 mg g-1) compared with hot water extraction (AG-1: 7.07 ± 0.15, PSJ-1: 7.39 ± 0.61 mg g-1). Maximum biomass, EPS and IPS production were observed when the initial pH was 6.7: AG-1: 12.92 ± 0.33, 209.70 ± 1.56, 32.62 ± 0.87; PSJ-1: 9.03 ± 0.24 g l-1 , 198.16 ± 0.85 mg g-1, 30.63 ± 1.96 mg g-1, respectively. The use 3.5 % coconut oil improved biomass, EPS, IPS production, which were 8.27 ± 0.09, 8.01 ± 0.01 g l-1 ; 1208.00 ± 8.60, 1110.40 ± 7.20 mg g-1; 32.43 ± 0.49, 29.74 ± 0.44, for AG-1 and PSJ-1, respectively. Both culture condition and oven drying methods had significant effects on H2O2 and radical scavenging activity, ABTS.+ radical activity, lipid peroxidation, and also had effects on total flavonoid and, total phenolic contents. The use of crude submerged liquid culture and oven drying on strains AG-1, PSJ-1 led to extracts with potent antioxidant activity, suggesting the therapeutic use of polysaccharides from strains AG-1, PSJ-1. |
| format | Article |
| id | doaj-art-0ab95c831960479588f4333f2fa4472f |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
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| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-0ab95c831960479588f4333f2fa4472f2025-08-20T02:52:56ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412020-12-01116233735010.14720/aas.2020.116.2.110419358Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)Dang Ngoc HUNG0Li Chun WANG1Liang Horng LAY2Vu Thi PHUONG3National Pingtung University of Science and Technology, Department of Tropical Agriculture and International Cooperation, Pingtung, 91201, TaiwanNational Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, TaiwanNational Pingtung University of Science and Technology, Department of Plant Industry, Pingtung, 91201, TaiwanThai Nguyen University of Sciences, Thai Nguyen, 3Faculty of Tourism, 24000, Vietnam.The mycelial biomass, antioxidant activity and production of extra- and intra-cellular polysaccharides production [EPS, IPS] of Cordyceps militaris strains AG-1, PSJ-1 were evaluated under different submerged liquid culture (SLC) conditions. At 24 0C mycelial biomass and polysaccharide production of AG-1, PSJ-1 was optimal using PVC media and static culture conditions; (AG-1: 21.85 ± 1.00; PSJ-1: 18.20 ± 1.84 g l-1 ), and oven drying at 40 oC (AG-1: 25.95 ± 0.84, PSJ-1: 23.55 ± 0.69 mg g-1) compared with hot water extraction (AG-1: 7.07 ± 0.15, PSJ-1: 7.39 ± 0.61 mg g-1). Maximum biomass, EPS and IPS production were observed when the initial pH was 6.7: AG-1: 12.92 ± 0.33, 209.70 ± 1.56, 32.62 ± 0.87; PSJ-1: 9.03 ± 0.24 g l-1 , 198.16 ± 0.85 mg g-1, 30.63 ± 1.96 mg g-1, respectively. The use 3.5 % coconut oil improved biomass, EPS, IPS production, which were 8.27 ± 0.09, 8.01 ± 0.01 g l-1 ; 1208.00 ± 8.60, 1110.40 ± 7.20 mg g-1; 32.43 ± 0.49, 29.74 ± 0.44, for AG-1 and PSJ-1, respectively. Both culture condition and oven drying methods had significant effects on H2O2 and radical scavenging activity, ABTS.+ radical activity, lipid peroxidation, and also had effects on total flavonoid and, total phenolic contents. The use of crude submerged liquid culture and oven drying on strains AG-1, PSJ-1 led to extracts with potent antioxidant activity, suggesting the therapeutic use of polysaccharides from strains AG-1, PSJ-1.https://journals.uni-lj.si/aas/article/view/12966antioxidant activitiescordyceps militarisextra-cellular polysaccharides and intra-cellular polysaccharidesoven dryingph |
| spellingShingle | Dang Ngoc HUNG Li Chun WANG Liang Horng LAY Vu Thi PHUONG Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) Acta Agriculturae Slovenica antioxidant activities cordyceps militaris extra-cellular polysaccharides and intra-cellular polysaccharides oven drying ph |
| title | Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) |
| title_full | Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) |
| title_fullStr | Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) |
| title_full_unstemmed | Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) |
| title_short | Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1) |
| title_sort | impact of different fermentation characteristics on the production of mycelial biomass extra cellular polysaccharides intra cellular polysaccharides and on the antioxidant activities of cordyceps militaris l fr strains ag 1 psj 1 |
| topic | antioxidant activities cordyceps militaris extra-cellular polysaccharides and intra-cellular polysaccharides oven drying ph |
| url | https://journals.uni-lj.si/aas/article/view/12966 |
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