Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors
An electrochemical potential-assisted functionalization strategy is used to immobilize resazurin (AZ) on multiwalled carbon nanotube surfaces in a physiological buffer leading to the formation of a resorufin/dihydro resorufin (RR/DRR) redox couple. The electrochemical characterizations that reveal t...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Electrochemistry Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248124001917 |
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| author | Balamurugan Thangavel Won Han Joong Ho Shin |
| author_facet | Balamurugan Thangavel Won Han Joong Ho Shin |
| author_sort | Balamurugan Thangavel |
| collection | DOAJ |
| description | An electrochemical potential-assisted functionalization strategy is used to immobilize resazurin (AZ) on multiwalled carbon nanotube surfaces in a physiological buffer leading to the formation of a resorufin/dihydro resorufin (RR/DRR) redox couple. The electrochemical characterizations that reveal the modified surface are surface-confined behavior with an electron transfer rate constant of 4.4 s−1. Thus modified RR/DRR redox couple was found to modulate the interfacial characteristics to the benefits of bio-electrocatalysis since the redox molecule has sensitivity to pH, negative redox potential, and selectivity to analytes. The hydrogen peroxide (H2O2) reduction and sensing performance of the AZ-modified electrode surface were evaluated. The experimental results revealed the direct detection of high concentrations of H2O2 at the electrified interface before the oxygen reduction potential. Furthermore, the designed sensor exhibited high selectivity for H2O2 even in the presence of interfering molecules in the solution. In addition, for the demonstration, the glucose oxidase enzymes were immobilized on carbon nanotubes modified with an RR/DRR redox couple, and the electron tunneling behavior was investigated. The developed sensor could be used for the reagent-less electrochemical biosensing of glucose up to 30 mM. Thus, the AZ-based redox electrode catalysts can be applied in diverse biosensor applications. |
| format | Article |
| id | doaj-art-0a250684b32e4da1a5af6a4600643759 |
| institution | Kabale University |
| issn | 1388-2481 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj-art-0a250684b32e4da1a5af6a46006437592025-01-12T05:24:24ZengElsevierElectrochemistry Communications1388-24812025-01-01170107848Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensorsBalamurugan Thangavel0Won Han1Joong Ho Shin2Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan 48513, Republic of KoreaMajor of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan 48513, Republic of Korea; Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of KoreaMajor of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan 48513, Republic of Korea; Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of Korea; Corresponding author at: Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan 48513, Republic of Korea.An electrochemical potential-assisted functionalization strategy is used to immobilize resazurin (AZ) on multiwalled carbon nanotube surfaces in a physiological buffer leading to the formation of a resorufin/dihydro resorufin (RR/DRR) redox couple. The electrochemical characterizations that reveal the modified surface are surface-confined behavior with an electron transfer rate constant of 4.4 s−1. Thus modified RR/DRR redox couple was found to modulate the interfacial characteristics to the benefits of bio-electrocatalysis since the redox molecule has sensitivity to pH, negative redox potential, and selectivity to analytes. The hydrogen peroxide (H2O2) reduction and sensing performance of the AZ-modified electrode surface were evaluated. The experimental results revealed the direct detection of high concentrations of H2O2 at the electrified interface before the oxygen reduction potential. Furthermore, the designed sensor exhibited high selectivity for H2O2 even in the presence of interfering molecules in the solution. In addition, for the demonstration, the glucose oxidase enzymes were immobilized on carbon nanotubes modified with an RR/DRR redox couple, and the electron tunneling behavior was investigated. The developed sensor could be used for the reagent-less electrochemical biosensing of glucose up to 30 mM. Thus, the AZ-based redox electrode catalysts can be applied in diverse biosensor applications.http://www.sciencedirect.com/science/article/pii/S1388248124001917ResazurinElectrochemical Redox probeSurface confinedHydrogen peroxide reductionGlucose oxidaseElectrochemical biosensor |
| spellingShingle | Balamurugan Thangavel Won Han Joong Ho Shin Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors Electrochemistry Communications Resazurin Electrochemical Redox probe Surface confined Hydrogen peroxide reduction Glucose oxidase Electrochemical biosensor |
| title | Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors |
| title_full | Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors |
| title_fullStr | Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors |
| title_full_unstemmed | Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors |
| title_short | Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors |
| title_sort | electrochemical modification and analytical exploration of resazurin as a redox active probe for electrochemical biosensors |
| topic | Resazurin Electrochemical Redox probe Surface confined Hydrogen peroxide reduction Glucose oxidase Electrochemical biosensor |
| url | http://www.sciencedirect.com/science/article/pii/S1388248124001917 |
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