miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway
ABSTRACT Background Our study aimed to explore the specific functions and potential mechanisms of miR‐224‐5p in non‐small cell lung cancer (NSCLC). Methods We first analyzed the expression of miR‐224‐5p in NSCLC patients and cell lines through the GEO database and qRT‐PCR analysis. Then, we used MTT...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15516 |
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| author | Jiao Tian Yiming He Zihui Zhang Yuxin Zhu Haixia Ren Liang Zhang Lei Li Wei Li Weidong Zhang Ting Xiao Honggang Zhou Xiaoping Li |
| author_facet | Jiao Tian Yiming He Zihui Zhang Yuxin Zhu Haixia Ren Liang Zhang Lei Li Wei Li Weidong Zhang Ting Xiao Honggang Zhou Xiaoping Li |
| author_sort | Jiao Tian |
| collection | DOAJ |
| description | ABSTRACT Background Our study aimed to explore the specific functions and potential mechanisms of miR‐224‐5p in non‐small cell lung cancer (NSCLC). Methods We first analyzed the expression of miR‐224‐5p in NSCLC patients and cell lines through the GEO database and qRT‐PCR analysis. Then, we used MTT assays, wound healing assays, Transwell assays, and western blotting to evaluate the effects of miR‐224‐5p on NSCLC cell proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT). Furthermore, we used a xenograft tumor model to evaluate the effect of miR‐224‐5p on NSCLC tumor growth. Potential binding targets of miR‐224‐5p were further identified through the target prediction databases, and the relationships between miR‐224‐5p, its targets, and downstream signaling pathways were further verified using luciferase reporter gene assays and western blotting. Results The GEO database and qRT‐PCR analysis indicated that miR‐224‐5p was significantly downregulated in NSCLC patients and cell lines. Functional assays indicated that inhibiting miR‐224‐5p could enhance the proliferation, migration, invasion, and EMT of NSCLC cells, as well as accelerate tumor growth. In contrast, overexpression of miR‐224‐5p inhibited these processes. We identified IL6ST (interleukin 6 signal transducer) as a binding target of miR‐224‐5p. We observed that miR‐224‐5p could bind to and inhibit IL6ST expression and JAK2/STAT3 signaling pathway, and the inhibition of NSCLC tumor growth and JAK2/STAT3 pathway by miR‐224‐5p could be reversed by IL6ST overexpression. Conclusion Our study demonstrated that miR‐224‐5p inhibited NSCLC by targeting IL6ST, thereby downregulating the JAK2/STAT3 signaling pathway. |
| format | Article |
| id | doaj-art-09dbf8a5c0af4a59b1d64127b5816727 |
| institution | Kabale University |
| issn | 1759-7706 1759-7714 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj-art-09dbf8a5c0af4a59b1d64127b58167272025-01-30T22:40:34ZengWileyThoracic Cancer1759-77061759-77142025-01-01162n/an/a10.1111/1759-7714.15516miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 PathwayJiao Tian0Yiming He1Zihui Zhang2Yuxin Zhu3Haixia Ren4Liang Zhang5Lei Li6Wei Li7Weidong Zhang8Ting Xiao9Honggang Zhou10Xiaoping Li11Department of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaDepartment of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine Nankai University Tianjin ChinaABSTRACT Background Our study aimed to explore the specific functions and potential mechanisms of miR‐224‐5p in non‐small cell lung cancer (NSCLC). Methods We first analyzed the expression of miR‐224‐5p in NSCLC patients and cell lines through the GEO database and qRT‐PCR analysis. Then, we used MTT assays, wound healing assays, Transwell assays, and western blotting to evaluate the effects of miR‐224‐5p on NSCLC cell proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT). Furthermore, we used a xenograft tumor model to evaluate the effect of miR‐224‐5p on NSCLC tumor growth. Potential binding targets of miR‐224‐5p were further identified through the target prediction databases, and the relationships between miR‐224‐5p, its targets, and downstream signaling pathways were further verified using luciferase reporter gene assays and western blotting. Results The GEO database and qRT‐PCR analysis indicated that miR‐224‐5p was significantly downregulated in NSCLC patients and cell lines. Functional assays indicated that inhibiting miR‐224‐5p could enhance the proliferation, migration, invasion, and EMT of NSCLC cells, as well as accelerate tumor growth. In contrast, overexpression of miR‐224‐5p inhibited these processes. We identified IL6ST (interleukin 6 signal transducer) as a binding target of miR‐224‐5p. We observed that miR‐224‐5p could bind to and inhibit IL6ST expression and JAK2/STAT3 signaling pathway, and the inhibition of NSCLC tumor growth and JAK2/STAT3 pathway by miR‐224‐5p could be reversed by IL6ST overexpression. Conclusion Our study demonstrated that miR‐224‐5p inhibited NSCLC by targeting IL6ST, thereby downregulating the JAK2/STAT3 signaling pathway.https://doi.org/10.1111/1759-7714.15516IL6STJAK2/STAT3 signaling pathwaymiR‐224‐5pNSCLC |
| spellingShingle | Jiao Tian Yiming He Zihui Zhang Yuxin Zhu Haixia Ren Liang Zhang Lei Li Wei Li Weidong Zhang Ting Xiao Honggang Zhou Xiaoping Li miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway Thoracic Cancer IL6ST JAK2/STAT3 signaling pathway miR‐224‐5p NSCLC |
| title | miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway |
| title_full | miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway |
| title_fullStr | miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway |
| title_full_unstemmed | miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway |
| title_short | miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway |
| title_sort | mir 224 5p suppresses non small cell lung cancer via il6st mediated regulation of the jak2 stat3 pathway |
| topic | IL6ST JAK2/STAT3 signaling pathway miR‐224‐5p NSCLC |
| url | https://doi.org/10.1111/1759-7714.15516 |
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