甲基转移酶3对人脑血管平滑肌细胞基因表达及增殖和迁移功能的影响 Effects of Methyltransferase 3 on Gene Expression, Proliferation, and Migration Functions of Human Brain Vascular Smooth Muscle Cells
目的 探究甲基转移酶3（methyltransferase 3，METTL3）对人脑血管平滑肌细胞（human brain vascular smooth muscle cells，HBVSMC）基因表达以及增殖和迁移功能的影响。 方法 采用小干扰RNA（small interfering RNA，shRNA）干扰技术，构建METTL3基因干扰载体并通过腺病毒感染HBVSMC，依据感染情况将其分为空白组（无病毒感染）、对照组（空载病毒感染）和干扰组（METTL3-shRNA病毒感染）。使用逆转录实时定量PCR（real-time reverse transcriptional PCR，RT-...
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Chinese Journal of Stroke
2025-01-01
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| Series: | Zhongguo cuzhong zazhi |
| Subjects: | |
| Online Access: | https://www.chinastroke.org.cn/CN/10.3969/j.issn.1673-5765.2025.01.013 |
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