Calibration and Storage of DNA Competitors Used for Contamination-Protected Competitive PCR

DNA fragments used as standards in competitive PCR were precisely calibrated using HPLC and commercially available DNA molecular mass markers. The accuracy of calibration was reflected by data that differed by only 2% from the mean when two independently purified and calibrated competitor preparatio...

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Bibliographic Details
Main Authors: Thomas Köhler, Anne-Katrin Rost, Harald Remke
Format: Article
Language:English
Published: Taylor & Francis Group 1997-10-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97234st07
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