Integration of therapeutic cargo into the human genome with programmable type V-K CAST

Integration of therapeutic cargo into the human genome with programmable type V-K CAST

Abstract CRISPR-associated (Cas) transposases (CAST) are RNA-guided systems capable of programmable integration of large segments of DNA without creating double-strand breaks. Engineered Cascade CAST function in human cells but are challenging to deploy due to the complexity of the targeting compone...

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Main Authors: Jason Liu, Daniela S. Aliaga Goltsman, Lisa M. Alexander, Khak Khak Khayi, Jennifer H. Hong, Drew T. Dunham, Christine A. Romano, Morayma M. Temoche-Diaz, Shailaja Chadha, Rodrigo Fregoso Ocampo, Jennifer Oki-O’Connell, Owen P. Janson, Keirstinne Turcios, Liliana Gonzalez-Osorio, Jared Muysson, Jenat Rahman, Sarah M. Laperriere, Audra E. Devoto, Cindy J. Castelle, Cristina N. Butterfield, Gregory J. Cost, Christopher T. Brown, Brian C. Thomas
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-57416-2
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https://doi.org/10.1038/s41467-025-57416-2

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