Chemical and biological antioxidant activity evaluation of extracts from berries pomaces
Berries and berries pomaces have a high content of antioxidant compounds, especially anthocyanins. This work evaluates the chemical (TPC, FRAP, DPPH, ABTS, and ORAC) and biological (assays of cytotoxicity and loss of mitochondrial membrane potential (Δψm) on HL60 and fibroblast cells) antioxidant ca...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225000915 |
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| author | Mauricio Vergara-Castro Carmen Soto-Maldonado María Elvira Zúñiga-Hansen Alejandra Arancibia-Díaz Paloma Fuentes Claudia Altamirano |
| author_facet | Mauricio Vergara-Castro Carmen Soto-Maldonado María Elvira Zúñiga-Hansen Alejandra Arancibia-Díaz Paloma Fuentes Claudia Altamirano |
| author_sort | Mauricio Vergara-Castro |
| collection | DOAJ |
| description | Berries and berries pomaces have a high content of antioxidant compounds, especially anthocyanins. This work evaluates the chemical (TPC, FRAP, DPPH, ABTS, and ORAC) and biological (assays of cytotoxicity and loss of mitochondrial membrane potential (Δψm) on HL60 and fibroblast cells) antioxidant capacity of hydroalcoholic and aqueous extracts from raspberry and maqui pomaces. Better chemical results were obtained with hydroalcoholic extracts. Raspberry extract has better results than maqui extract, except for anthocyanin content and ORAC activity that was similar.A maximum of 75 % and 38 % of inhibition of HL60 cell growth, respectively, was observed with hydroalcoholic and aqueous extracts at 0.15 mg/mL. Fibroblast growth was inhibited only with raspberry pomace extract. Mostly, extracts allow cell growth under oxidative conditions, observing a protective effect equivalent to using a reference antioxidant (based on Δψm). This study therefore lays the groundwork for the biological evaluation of the use of extracts of maqui and raspberry pomace, with particular attention to their possible anticancer properties. |
| format | Article |
| id | doaj-art-08429acdf43d481db7c42af4317cc2ee |
| institution | DOAJ |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-08429acdf43d481db7c42af4317cc2ee2025-08-20T02:40:02ZengElsevierApplied Food Research2772-50222025-06-015110078110.1016/j.afres.2025.100781Chemical and biological antioxidant activity evaluation of extracts from berries pomacesMauricio Vergara-Castro0Carmen Soto-Maldonado1María Elvira Zúñiga-Hansen2Alejandra Arancibia-Díaz3Paloma Fuentes4Claudia Altamirano5Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile; Centro Regional de Estudios en Alimentos Saludables, Avenida Universidad 330, Curauma-Placilla, Valparaíso, Chile; Corresponding author at: Centro Regional de Estudios en Alimentos Saludables, Avenida Universidad 330, Curauma-Placilla, Valparaíso, Chile.Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile; Centro Regional de Estudios en Alimentos Saludables, Avenida Universidad 330, Curauma-Placilla, Valparaíso, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile; IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, ChileIMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile; Centro Regional de Estudios en Alimentos Saludables, Avenida Universidad 330, Curauma-Placilla, Valparaíso, Chile; IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, ChileBerries and berries pomaces have a high content of antioxidant compounds, especially anthocyanins. This work evaluates the chemical (TPC, FRAP, DPPH, ABTS, and ORAC) and biological (assays of cytotoxicity and loss of mitochondrial membrane potential (Δψm) on HL60 and fibroblast cells) antioxidant capacity of hydroalcoholic and aqueous extracts from raspberry and maqui pomaces. Better chemical results were obtained with hydroalcoholic extracts. Raspberry extract has better results than maqui extract, except for anthocyanin content and ORAC activity that was similar.A maximum of 75 % and 38 % of inhibition of HL60 cell growth, respectively, was observed with hydroalcoholic and aqueous extracts at 0.15 mg/mL. Fibroblast growth was inhibited only with raspberry pomace extract. Mostly, extracts allow cell growth under oxidative conditions, observing a protective effect equivalent to using a reference antioxidant (based on Δψm). This study therefore lays the groundwork for the biological evaluation of the use of extracts of maqui and raspberry pomace, with particular attention to their possible anticancer properties.http://www.sciencedirect.com/science/article/pii/S2772502225000915Agro-industrial wastes revaluationBerries pomacesHydroalcoholic and aqueous extractsHL60 cell lineChemical and biological antioxidant activity |
| spellingShingle | Mauricio Vergara-Castro Carmen Soto-Maldonado María Elvira Zúñiga-Hansen Alejandra Arancibia-Díaz Paloma Fuentes Claudia Altamirano Chemical and biological antioxidant activity evaluation of extracts from berries pomaces Applied Food Research Agro-industrial wastes revaluation Berries pomaces Hydroalcoholic and aqueous extracts HL60 cell line Chemical and biological antioxidant activity |
| title | Chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| title_full | Chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| title_fullStr | Chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| title_full_unstemmed | Chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| title_short | Chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| title_sort | chemical and biological antioxidant activity evaluation of extracts from berries pomaces |
| topic | Agro-industrial wastes revaluation Berries pomaces Hydroalcoholic and aqueous extracts HL60 cell line Chemical and biological antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225000915 |
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