Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens
Introduction: Probiotic and postbiotic potential of thirty-two strains of lactic acid bacteria (LAB), obtained earlier from artisanal dairy sources in Pakistan, have been investigated against major multi-drug resistant (MDR) and food borne pathogenic bacteria. Methodology: LAB strains were ident...
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The Journal of Infection in Developing Countries
2021-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/13404 |
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| author | Nazar Hussain Muhammad Tariq Per Erik Joakim Saris Arsalan Zaidi |
| author_facet | Nazar Hussain Muhammad Tariq Per Erik Joakim Saris Arsalan Zaidi |
| author_sort | Nazar Hussain |
| collection | DOAJ |
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Introduction: Probiotic and postbiotic potential of thirty-two strains of lactic acid bacteria (LAB), obtained earlier from artisanal dairy sources in Pakistan, have been investigated against major multi-drug resistant (MDR) and food borne pathogenic bacteria.
Methodology: LAB strains were identified by 16S rRNA gene sequencing and their antibacterial activity was assessed by the microdilution method. Four LAB isolates, Weissella confusa PL6, Enterococcus faecium PL7, and Lactobacillus delbrueckii PL11 and PL13 were shortlisted. Their ability to degrade lactose and safety for human consumption in terms of hemolysis and antibiotic susceptibility were assessed in vitro. The antibacterial components in the cell-free supernatants (CFSs) of isolate cultures were characterized biochemically by HPLC.
Results: Acid neutralization but not protease treatment abolished the antibacterial activity of CFSs. Lactic, acetic and propionic acids were the main acids in the CFSs, and acid production peaked in the stationary phase of growth. The antibacterial activity of the LAB cultures resulted from secretion of organic acids that lowered the pH. The strains exhibited variable ability to degrade lactose and were non-hemolytic and susceptible to the most common antibiotics.
Conclusions: These LAB strains are probiotic candidates for further investigation of their postbiotic role in naturally preserving processed foods and for attenuation of lactose intolerance.
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| format | Article |
| id | doaj-art-0819beb597344a0589ba8f1044bd0f30 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0819beb597344a0589ba8f1044bd0f302025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-01-01150110.3855/jidc.13404Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens Nazar Hussain0Muhammad Tariq1Per Erik Joakim Saris2Arsalan Zaidi3National Probiotics Lab-HBD, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, PakistanNational Probiotics Lab-HBD, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, PakistanDepartment of Microbiology, University of Helsinki, Helsinki, FinlandNational Probiotics Lab-HBD, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Introduction: Probiotic and postbiotic potential of thirty-two strains of lactic acid bacteria (LAB), obtained earlier from artisanal dairy sources in Pakistan, have been investigated against major multi-drug resistant (MDR) and food borne pathogenic bacteria. Methodology: LAB strains were identified by 16S rRNA gene sequencing and their antibacterial activity was assessed by the microdilution method. Four LAB isolates, Weissella confusa PL6, Enterococcus faecium PL7, and Lactobacillus delbrueckii PL11 and PL13 were shortlisted. Their ability to degrade lactose and safety for human consumption in terms of hemolysis and antibiotic susceptibility were assessed in vitro. The antibacterial components in the cell-free supernatants (CFSs) of isolate cultures were characterized biochemically by HPLC. Results: Acid neutralization but not protease treatment abolished the antibacterial activity of CFSs. Lactic, acetic and propionic acids were the main acids in the CFSs, and acid production peaked in the stationary phase of growth. The antibacterial activity of the LAB cultures resulted from secretion of organic acids that lowered the pH. The strains exhibited variable ability to degrade lactose and were non-hemolytic and susceptible to the most common antibiotics. Conclusions: These LAB strains are probiotic candidates for further investigation of their postbiotic role in naturally preserving processed foods and for attenuation of lactose intolerance. https://jidc.org/index.php/journal/article/view/13404antagonismmulti-drug resistanceorganic acidslactic acid bacteriaprobioticslactose intolerance |
| spellingShingle | Nazar Hussain Muhammad Tariq Per Erik Joakim Saris Arsalan Zaidi Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens Journal of Infection in Developing Countries antagonism multi-drug resistance organic acids lactic acid bacteria probiotics lactose intolerance |
| title | Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| title_full | Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| title_fullStr | Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| title_full_unstemmed | Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| title_short | Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| title_sort | evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens |
| topic | antagonism multi-drug resistance organic acids lactic acid bacteria probiotics lactose intolerance |
| url | https://jidc.org/index.php/journal/article/view/13404 |
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