Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响)
Quinclorac-resistant Echinochloa spp. can promote the growth and reproduction of Laodelphax striatellus, which is closely related to the microbiota in vivo. In this study, 16S rRNA gene amplicon sequencing was used to analyze and compare the structural characteristics of microbiota in Laodelphax str...
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Zhejiang University Press
2025-04-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://doi.org/10.3785/j.issn.1008-9209.2025.02.111 |
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| author | 郭嘉雯(GUO Jiawen) 皮艺萌(PI Yimeng) 鲁艳辉(LU Yanhui) 杨霞(YANG Xia) 许益鹏(XU Yipeng) 吕仲贤(LÜ Zhongxian) 李永丰(LI Yongfeng) 徐红星(XU Hongxing) |
| author_facet | 郭嘉雯(GUO Jiawen) 皮艺萌(PI Yimeng) 鲁艳辉(LU Yanhui) 杨霞(YANG Xia) 许益鹏(XU Yipeng) 吕仲贤(LÜ Zhongxian) 李永丰(LI Yongfeng) 徐红星(XU Hongxing) |
| author_sort | 郭嘉雯(GUO Jiawen) |
| collection | DOAJ |
| description | Quinclorac-resistant Echinochloa spp. can promote the growth and reproduction of Laodelphax striatellus, which is closely related to the microbiota in vivo. In this study, 16S rRNA gene amplicon sequencing was used to analyze and compare the structural characteristics of microbiota in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp. and rice, and to predict the functions of the bacterial communities, in order to investigate the effect of quinclorac-resistant Echinochloa spp. on the structure and function of microbiota in Laodelphax striatellus. The results showed that feeding on quinclorac-resistant Echinochloa spp. reduced the relative abundance of Proteobacteria microbiota but increased that of Bacteroidota microbiota. At the genus level, the relative abundance of Wolbachia microbiota exhibited a declining trend, whereas Chryseobacterium and Stenotrophomonas microbiota showed increasing trends in relative abundance, although no significant differences in alpha diversity index were observed. Functional prediction analysis revealed that the in vivo bacterial communities in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp. exhibited significantly elevated gene abundances in pathways related to energy metabolism, immune system, and nervous system, whereas resistance-related gene abundance decreased in those fed on quinclorac-resistant Echinochloa crus-pavonis. The results of this study demonstrate that feeding on quinclorac-resistant Echinochloa spp. alters the microbiota structure and affects the gene function of microbiota in Laodelphax striatellus, providing a theoretical foundation for elucidating the dynamic regulatory mechanism underlying the response of the microbiota in Laodelphax striatellus to quinclorac-resistant Echinochloa spp.
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二氯喹啉酸抗性稗草能促进灰飞虱的生长和繁殖，而这一过程与其体内微生物群落密切相关。本研究利用16S rRNA基因扩增子测序技术，分析和比较了取食二氯喹啉酸抗性稗草、敏感稗草以及水稻的灰飞虱体内菌群结构特征，并预测了细菌菌群的功能，以探究二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响。结果表明：取食二氯喹啉酸抗性稗草降低了灰飞虱体内变形菌门菌群的相对丰度，而增加了拟杆菌门菌群的相对丰度。在属水平上，沃尔巴克菌属菌群相对丰度呈下降趋势，而金黄杆菌属和寡养单胞菌属菌群相对丰度呈上升趋势，但菌群α多样性指数无显著差异。功能预测结果显示，取食二氯喹啉酸抗性稗草和敏感稗草的灰飞虱体内细菌菌群在能量代谢、免疫系统、神经系统等通路上基因丰度显著上升，而取食二氯喹啉酸抗性孔雀稗的灰飞虱耐药性相关基因丰度下降。本研究结果揭示了取食二氯喹啉酸抗性稗草可改变灰飞虱体内微生物群落结构并影响菌群基因功能，为深入阐明灰飞虱微生物群落响应抗性稗草的动态调控机制提供了理论依据。 |
| format | Article |
| id | doaj-art-07ee57818c404233a2e68b33b4af51c6 |
| institution | Kabale University |
| issn | 2097-5155 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-07ee57818c404233a2e68b33b4af51c62025-08-20T04:02:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版2097-51552025-04-0151227729010.3785/j.issn.1008-9209.2025.02.11110089209Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响)郭嘉雯(GUO Jiawen)0https://orcid.org/0000-0001-6743-1874皮艺萌(PI Yimeng)1鲁艳辉(LU Yanhui)2杨霞(YANG Xia)3许益鹏(XU Yipeng)4吕仲贤(LÜ Zhongxian)5李永丰(LI Yongfeng)6徐红星(XU Hongxing)7https://orcid.org/0009-0007-5940-3700 1 State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China( 1 浙江省农业科学院植物保护与微生物研究所，农产品质量安全全国重点实验室，浙江 杭州 310021) 1 State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China( 1 浙江省农业科学院植物保护与微生物研究所，农产品质量安全全国重点实验室，浙江 杭州 310021) 1 State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China( 1 浙江省农业科学院植物保护与微生物研究所，农产品质量安全全国重点实验室，浙江 杭州 310021) 3 Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China( 3 江苏省农业科学院种质资源与生物技术研究所，江苏 南京 210014) 2 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang, China( 2 中国计量大学生命科学学院，浙江省生物计量及检验检疫技术重点实验室，浙江 杭州 310018) 1 State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China( 1 浙江省农业科学院植物保护与微生物研究所，农产品质量安全全国重点实验室，浙江 杭州 310021) 3 Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China( 3 江苏省农业科学院种质资源与生物技术研究所，江苏 南京 210014) 1 State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China( 1 浙江省农业科学院植物保护与微生物研究所，农产品质量安全全国重点实验室，浙江 杭州 310021)Quinclorac-resistant Echinochloa spp. can promote the growth and reproduction of Laodelphax striatellus, which is closely related to the microbiota in vivo. In this study, 16S rRNA gene amplicon sequencing was used to analyze and compare the structural characteristics of microbiota in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp. and rice, and to predict the functions of the bacterial communities, in order to investigate the effect of quinclorac-resistant Echinochloa spp. on the structure and function of microbiota in Laodelphax striatellus. The results showed that feeding on quinclorac-resistant Echinochloa spp. reduced the relative abundance of Proteobacteria microbiota but increased that of Bacteroidota microbiota. At the genus level, the relative abundance of Wolbachia microbiota exhibited a declining trend, whereas Chryseobacterium and Stenotrophomonas microbiota showed increasing trends in relative abundance, although no significant differences in alpha diversity index were observed. Functional prediction analysis revealed that the in vivo bacterial communities in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp. exhibited significantly elevated gene abundances in pathways related to energy metabolism, immune system, and nervous system, whereas resistance-related gene abundance decreased in those fed on quinclorac-resistant Echinochloa crus-pavonis. The results of this study demonstrate that feeding on quinclorac-resistant Echinochloa spp. alters the microbiota structure and affects the gene function of microbiota in Laodelphax striatellus, providing a theoretical foundation for elucidating the dynamic regulatory mechanism underlying the response of the microbiota in Laodelphax striatellus to quinclorac-resistant Echinochloa spp. . 二氯喹啉酸抗性稗草能促进灰飞虱的生长和繁殖，而这一过程与其体内微生物群落密切相关。本研究利用16S rRNA基因扩增子测序技术，分析和比较了取食二氯喹啉酸抗性稗草、敏感稗草以及水稻的灰飞虱体内菌群结构特征，并预测了细菌菌群的功能，以探究二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响。结果表明：取食二氯喹啉酸抗性稗草降低了灰飞虱体内变形菌门菌群的相对丰度，而增加了拟杆菌门菌群的相对丰度。在属水平上，沃尔巴克菌属菌群相对丰度呈下降趋势，而金黄杆菌属和寡养单胞菌属菌群相对丰度呈上升趋势，但菌群α多样性指数无显著差异。功能预测结果显示，取食二氯喹啉酸抗性稗草和敏感稗草的灰飞虱体内细菌菌群在能量代谢、免疫系统、神经系统等通路上基因丰度显著上升，而取食二氯喹啉酸抗性孔雀稗的灰飞虱耐药性相关基因丰度下降。本研究结果揭示了取食二氯喹啉酸抗性稗草可改变灰飞虱体内微生物群落结构并影响菌群基因功能，为深入阐明灰飞虱微生物群落响应抗性稗草的动态调控机制提供了理论依据。https://doi.org/10.3785/j.issn.1008-9209.2025.02.111除草剂抗性(herbicide resistance)稗草(echinochloa spp.)灰飞虱(laodelphax striatellus)微生物群落(microbiota) |
| spellingShingle | 郭嘉雯(GUO Jiawen) 皮艺萌(PI Yimeng) 鲁艳辉(LU Yanhui) 杨霞(YANG Xia) 许益鹏(XU Yipeng) 吕仲贤(LÜ Zhongxian) 李永丰(LI Yongfeng) 徐红星(XU Hongxing) Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) 浙江大学学报. 农业与生命科学版 除草剂抗性(herbicide resistance) 稗草(echinochloa spp.) 灰飞虱(laodelphax striatellus) 微生物群落(microbiota) |
| title | Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) |
| title_full | Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) |
| title_fullStr | Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) |
| title_full_unstemmed | Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) |
| title_short | Effect of quinclorac-resistant Echinochloa spp. on the microbiota structure and function of Laodelphax striatellus(二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响) |
| title_sort | effect of quinclorac resistant echinochloa spp on the microbiota structure and function of laodelphax striatellus 二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响 |
| topic | 除草剂抗性(herbicide resistance) 稗草(echinochloa spp.) 灰飞虱(laodelphax striatellus) 微生物群落(microbiota) |
| url | https://doi.org/10.3785/j.issn.1008-9209.2025.02.111 |
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