Influence of Electroporation Timing on CRISPR/Cas-Mediated Multiple Gene Editing in Buffalo Embryos

Influence of Electroporation Timing on CRISPR/Cas-Mediated Multiple Gene Editing in Buffalo Embryos

Gene editing in large animals like buffalo is challenged by mosaicism, where cells contain both wild-type and mutant alleles, complicating the creation of genetically modified F0 animals in a single step. Traditionally, electroporation is performed on zygotes post in vitro fertilization (IVF),...

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Bibliographic Details
Main Authors: Dipankar Sardar, Md. Taslim Hossain, Rudranil Baidya, Adnan Habib
Format: Article
Language:English
Published: Faculty of Veterinary Medicine, Chiang Mai University 2024-10-01
Series:Veterinary Integrative Sciences
Online Access:https://he02.tci-thaijo.org/index.php/vis/article/view/271085
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