Influence of Electroporation Timing on CRISPR/Cas-Mediated Multiple Gene Editing in Buffalo Embryos
Gene editing in large animals like buffalo is challenged by mosaicism, where cells contain both wild-type and mutant alleles, complicating the creation of genetically modified F0 animals in a single step. Traditionally, electroporation is performed on zygotes post in vitro fertilization (IVF),...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Chiang Mai University
2024-10-01
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| Series: | Veterinary Integrative Sciences |
| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/271085 |
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