Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration
The aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2011/175362 |
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| author | S. Saska H. S. Barud A. M. M. Gaspar R. Marchetto S. J. L. Ribeiro Y. Messaddeq |
| author_facet | S. Saska H. S. Barud A. M. M. Gaspar R. Marchetto S. J. L. Ribeiro Y. Messaddeq |
| author_sort | S. Saska |
| collection | DOAJ |
| description | The aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4. BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40%–50% of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration. |
| format | Article |
| id | doaj-art-077da4bb33714956bcbf34b41b37836f |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-077da4bb33714956bcbf34b41b37836f2025-02-03T01:31:41ZengWileyInternational Journal of Biomaterials1687-87871687-87952011-01-01201110.1155/2011/175362175362Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone RegenerationS. Saska0H. S. Barud1A. M. M. Gaspar2R. Marchetto3S. J. L. Ribeiro4Y. Messaddeq5Institute of Chemistry, University Estadual Paulista—UNESP, CP 355, 14-801-970 Araraquara, SP, BrazilInstitute of Chemistry, University Estadual Paulista—UNESP, CP 355, 14-801-970 Araraquara, SP, BrazilDepartment of Morphology, Dental School, University Estadual Paulista—UNESP, Rua Humaitá, 1680, 14-801-903 Araraquara, SP, BrazilInstitute of Chemistry, University Estadual Paulista—UNESP, CP 355, 14-801-970 Araraquara, SP, BrazilInstitute of Chemistry, University Estadual Paulista—UNESP, CP 355, 14-801-970 Araraquara, SP, BrazilInstitute of Chemistry, University Estadual Paulista—UNESP, CP 355, 14-801-970 Araraquara, SP, BrazilThe aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4. BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40%–50% of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration.http://dx.doi.org/10.1155/2011/175362 |
| spellingShingle | S. Saska H. S. Barud A. M. M. Gaspar R. Marchetto S. J. L. Ribeiro Y. Messaddeq Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration International Journal of Biomaterials |
| title | Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration |
| title_full | Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration |
| title_fullStr | Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration |
| title_full_unstemmed | Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration |
| title_short | Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration |
| title_sort | bacterial cellulose hydroxyapatite nanocomposites for bone regeneration |
| url | http://dx.doi.org/10.1155/2011/175362 |
| work_keys_str_mv | AT ssaska bacterialcellulosehydroxyapatitenanocompositesforboneregeneration AT hsbarud bacterialcellulosehydroxyapatitenanocompositesforboneregeneration AT ammgaspar bacterialcellulosehydroxyapatitenanocompositesforboneregeneration AT rmarchetto bacterialcellulosehydroxyapatitenanocompositesforboneregeneration AT sjlribeiro bacterialcellulosehydroxyapatitenanocompositesforboneregeneration AT ymessaddeq bacterialcellulosehydroxyapatitenanocompositesforboneregeneration |