Bioinformatic prediction, annotation and experimental validation of conserved microRNAs in Lactuca sativa Linn

Bioinformatic prediction, annotation and experimental validation of conserved microRNAs in Lactuca sativa Linn

Abstract MicroRNAs (miRNAs) are non-coding RNAs that play crucial role in post-transcriptional gene regulation, with lengths ranging from 18 to 26 nucleotides (nt). These endogenously expressed molecules exhibit significant evolutionary conservation, a feature vital for identifying new conserved miR...

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Main Authors: Shehnaz Shair Qambrani, Muhammad Naeem Shahwani, Iftekhar Ahmed Baloch, Shahjahan Shabir Ahmed Rana, Asma Abro
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Bioinformatic identification
Conserved miRNAs
Lettuce
miRNA targets
RT-PCR validation
Online Access:https://doi.org/10.1038/s41598-025-88689-8
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https://doi.org/10.1038/s41598-025-88689-8

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