Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy
Abstract Background Prostate cancer remains a significant health challenge, as conventional treatments often fail to provide long-term benefits in the advanced stages. This study explored the potential of mRNA vaccines as novel therapeutic strategies, specifically focusing on the combination of 5T4...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Journal of Nanobiotechnology |
| Online Access: | https://doi.org/10.1186/s12951-025-03607-4 |
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| author | Fei Cao Yuandong Xu Yupeng Guan Kexin Zhang Haowei Qiu Zhen Xu Yunru He Ze Xiu Xiao Gao-feng Zha Jun Pang |
| author_facet | Fei Cao Yuandong Xu Yupeng Guan Kexin Zhang Haowei Qiu Zhen Xu Yunru He Ze Xiu Xiao Gao-feng Zha Jun Pang |
| author_sort | Fei Cao |
| collection | DOAJ |
| description | Abstract Background Prostate cancer remains a significant health challenge, as conventional treatments often fail to provide long-term benefits in the advanced stages. This study explored the potential of mRNA vaccines as novel therapeutic strategies, specifically focusing on the combination of 5T4 and CD70 mRNA delivered via lipid nanoparticles (LNPs). 5T4, which is highly expressed in various tumors but minimally expressed in normal tissues, is an ideal target for immunotherapy. CD70 was selected for its capacity to enhance T cell activation and augment the overall immune response. Results Our findings demonstrated that the combination of 5T4 and CD70 mRNA-LNPs significantly enhanced both humoral and cellular immunity, resulting in improved tumor suppression and prolonged survival in a prostate cancer mouse model. Mechanistically, these LNPs increased the immune responses, including elevated 5T4-specific antibodies, enhanced CD8 + T cell activity, and more active natural killer (NK) cells. Conclusions These results suggest that this combined mRNA vaccine strategy may offer more effective and durable treatments for prostate cancer and that CD70 mRNA, as an immune activator, has broader potential for cancer immunotherapy. Graphical Abstract |
| format | Article |
| id | doaj-art-06597f58328745909b2f6c686f4c0a49 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-06597f58328745909b2f6c686f4c0a492025-08-20T04:02:55ZengBMCJournal of Nanobiotechnology1477-31552025-07-0123112010.1186/s12951-025-03607-4Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapyFei Cao0Yuandong Xu1Yupeng Guan2Kexin Zhang3Haowei Qiu4Zhen Xu5Yunru He6Ze Xiu Xiao7Gao-feng Zha8Jun Pang9Department of Urology, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen UniversityDepartment of Urology, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen UniversityDepartment of Urology, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen UniversityResearch and development center, Shenzhen MagicRNA BiotechScientific Research Center, the Seventh Affiliated Hospital, Sun Yat-sen UniversityDepartment of Urology, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen UniversityScientific Research Center, the Seventh Affiliated Hospital, Sun Yat-sen UniversityResearch and development center, Shenzhen MagicRNA BiotechScientific Research Center, the Seventh Affiliated Hospital, Sun Yat-sen UniversityDepartment of Urology, Pelvic Floor Disorders Center, The Seventh Affiliated Hospital, Sun Yat-sen UniversityAbstract Background Prostate cancer remains a significant health challenge, as conventional treatments often fail to provide long-term benefits in the advanced stages. This study explored the potential of mRNA vaccines as novel therapeutic strategies, specifically focusing on the combination of 5T4 and CD70 mRNA delivered via lipid nanoparticles (LNPs). 5T4, which is highly expressed in various tumors but minimally expressed in normal tissues, is an ideal target for immunotherapy. CD70 was selected for its capacity to enhance T cell activation and augment the overall immune response. Results Our findings demonstrated that the combination of 5T4 and CD70 mRNA-LNPs significantly enhanced both humoral and cellular immunity, resulting in improved tumor suppression and prolonged survival in a prostate cancer mouse model. Mechanistically, these LNPs increased the immune responses, including elevated 5T4-specific antibodies, enhanced CD8 + T cell activity, and more active natural killer (NK) cells. Conclusions These results suggest that this combined mRNA vaccine strategy may offer more effective and durable treatments for prostate cancer and that CD70 mRNA, as an immune activator, has broader potential for cancer immunotherapy. Graphical Abstracthttps://doi.org/10.1186/s12951-025-03607-4 |
| spellingShingle | Fei Cao Yuandong Xu Yupeng Guan Kexin Zhang Haowei Qiu Zhen Xu Yunru He Ze Xiu Xiao Gao-feng Zha Jun Pang Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy Journal of Nanobiotechnology |
| title | Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy |
| title_full | Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy |
| title_fullStr | Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy |
| title_full_unstemmed | Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy |
| title_short | Enhancing the potency of 5T4 mRNA vaccine by CD70 mRNA-LNPs through ADCC and T cell boosting in prostate cancer therapy |
| title_sort | enhancing the potency of 5t4 mrna vaccine by cd70 mrna lnps through adcc and t cell boosting in prostate cancer therapy |
| url | https://doi.org/10.1186/s12951-025-03607-4 |
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