Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines
Targeted gene disruption is a powerful tool for studying gene function in cells and animals. In addition, this technology includes a potential to correct disease-causing mutations. However, constructing targeting vectors is a laborious step in the gene-targeting strategy, even apart from the low eff...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2006-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112233 |
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