Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice
Summary: Angiopoietin-like protein 8 (Angptl8), expressed in the liver and adipocytes, forms a complex with Angptl3 or Angptl4, which regulates lipoprotein lipase and triglyceride metabolism. However, the precise functions of adipocyte Angptl8 remain elusive. Here we report that adipocyte-specific i...
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Elsevier
2024-12-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224025173 |
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| author | Anindya Ghosh Isabelle Chénier Yat Hei Leung Abel K. Oppong Marie-Line Peyot S. R. Murthy Madiraju Irina Al-Khairi Jehad Abubaker Fahd Al-Mulla Marc Prentki Mohamed Abu-Farha |
| author_facet | Anindya Ghosh Isabelle Chénier Yat Hei Leung Abel K. Oppong Marie-Line Peyot S. R. Murthy Madiraju Irina Al-Khairi Jehad Abubaker Fahd Al-Mulla Marc Prentki Mohamed Abu-Farha |
| author_sort | Anindya Ghosh |
| collection | DOAJ |
| description | Summary: Angiopoietin-like protein 8 (Angptl8), expressed in the liver and adipocytes, forms a complex with Angptl3 or Angptl4, which regulates lipoprotein lipase and triglyceride metabolism. However, the precise functions of adipocyte Angptl8 remain elusive. Here we report that adipocyte-specific inducible Angptl8-knockout (AT-A8-KO) male mice on normal diet showed minor phenotypic changes, but after a high-fat high fructose (HFHF) diet, exhibited decreased body weight gain and glycemia, elevated rectal temperature and early dark phase energy expenditure compared to the Cre controls. AT-A8-KO mice also displayed improved glucose tolerance, a trend for better insulin sensitivity, improved insulin-stimulated glucose uptake in adipose tissues, and reduced visceral adipose tissue crown-like structures, plasma MCP-1 and leptin levels. The results indicate the importance of adipose Angptl8 in the context of nutri-stress and obesity, as its deletion in mice promotes a metabolically healthy obese phenotype by slightly ameliorating obesity, improving glucose and energy homeostasis, and mitigating inflammation. |
| format | Article |
| id | doaj-art-050289fe5d4542d5980451a7aff949a3 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-050289fe5d4542d5980451a7aff949a32025-08-20T02:39:48ZengElsevieriScience2589-00422024-12-01271211129210.1016/j.isci.2024.111292Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in miceAnindya Ghosh0Isabelle Chénier1Yat Hei Leung2Abel K. Oppong3Marie-Line Peyot4S. R. Murthy Madiraju5Irina Al-Khairi6Jehad Abubaker7Fahd Al-Mulla8Marc Prentki9Mohamed Abu-Farha10Departments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, CanadaBiochemistry and Molecular Biology Department, Dasman Diabetes Institute, Dasman 15462, KuwaitBiochemistry and Molecular Biology Department, Dasman Diabetes Institute, Dasman 15462, KuwaitTranslational Research Department, Dasman Diabetes Institute, Dasman 15462, KuwaitDepartments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche Du Centre Hospitalier de L’Université de Montréal (CRCHUM), Montréal, QC, Canada; Corresponding authorBiochemistry and Molecular Biology Department, Dasman Diabetes Institute, Dasman 15462, Kuwait; Translational Research Department, Dasman Diabetes Institute, Dasman 15462, Kuwait; Corresponding authorSummary: Angiopoietin-like protein 8 (Angptl8), expressed in the liver and adipocytes, forms a complex with Angptl3 or Angptl4, which regulates lipoprotein lipase and triglyceride metabolism. However, the precise functions of adipocyte Angptl8 remain elusive. Here we report that adipocyte-specific inducible Angptl8-knockout (AT-A8-KO) male mice on normal diet showed minor phenotypic changes, but after a high-fat high fructose (HFHF) diet, exhibited decreased body weight gain and glycemia, elevated rectal temperature and early dark phase energy expenditure compared to the Cre controls. AT-A8-KO mice also displayed improved glucose tolerance, a trend for better insulin sensitivity, improved insulin-stimulated glucose uptake in adipose tissues, and reduced visceral adipose tissue crown-like structures, plasma MCP-1 and leptin levels. The results indicate the importance of adipose Angptl8 in the context of nutri-stress and obesity, as its deletion in mice promotes a metabolically healthy obese phenotype by slightly ameliorating obesity, improving glucose and energy homeostasis, and mitigating inflammation.http://www.sciencedirect.com/science/article/pii/S2589004224025173Biochemical mechanism |
| spellingShingle | Anindya Ghosh Isabelle Chénier Yat Hei Leung Abel K. Oppong Marie-Line Peyot S. R. Murthy Madiraju Irina Al-Khairi Jehad Abubaker Fahd Al-Mulla Marc Prentki Mohamed Abu-Farha Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice iScience Biochemical mechanism |
| title | Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| title_full | Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| title_fullStr | Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| title_full_unstemmed | Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| title_short | Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| title_sort | adipocyte angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice |
| topic | Biochemical mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224025173 |
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