Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.

Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.

Patients with liver cirrhosis are susceptible to fungal infections. Due to low sensitivity of culture-based methods, we applied a real-time PCR assay targeting the 18S rRNA gene in combination with direct sequencing and terminal-restriction fragment length polymorphism (T-RFLP) in order to establish...

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Main Authors: Sandra Krohn, Katharina Zeller, Stephan Böhm, Antonis Chatzinotas, Hauke Harms, Jan Hartmann, Anett Heidtmann, Adam Herber, Thorsten Kaiser, Maud Treuheit, Albrecht Hoffmeister, Thomas Berg, Cornelius Engelmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0197319/1/pone.0197319.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210218%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210218T134555Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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