Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice
In this study, we assessed the feasibility of using positron emission tomography (PET) and the tracer [ 11 C]OMAR ([ 11 C]JHU75528), an analogue of rimonabant, to study the brain cannabinoid type 1 (CB1) receptor system. Wild-type (WT) andCB1 knockout (KO) animals were imaged at baseline and after p...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2011-11-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00019 |
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| _version_ | 1841563118429274112 |
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| author | Raúl Herance Santiago Rojas Sergio Abad Xavier Jiménez Juan Domingo Gispert Olga Millán Elena Martín-García Aurelijus Burokas Miquel Àngel Serra Rafael Maldonado Deborah Pareto |
| author_facet | Raúl Herance Santiago Rojas Sergio Abad Xavier Jiménez Juan Domingo Gispert Olga Millán Elena Martín-García Aurelijus Burokas Miquel Àngel Serra Rafael Maldonado Deborah Pareto |
| author_sort | Raúl Herance |
| collection | DOAJ |
| description | In this study, we assessed the feasibility of using positron emission tomography (PET) and the tracer [ 11 C]OMAR ([ 11 C]JHU75528), an analogue of rimonabant, to study the brain cannabinoid type 1 (CB1) receptor system. Wild-type (WT) andCB1 knockout (KO) animals were imaged at baseline and after pretreatment with blocking doses of rimonabant. Brain uptake in WT animals was higher (50%) than in KO animals in baseline conditions. After pretreatment with rimonabant, WT uptake lowered to the level of KO animals. The results of this study support the feasibility of using PET with the radiotracer [ 11 C]JHU75528 to image the brain CB1 receptor system in mice. In addition, this methodology can be used to assess the effect of new drugs in preclinical studies using genetically manipulated animals. |
| format | Article |
| id | doaj-art-0210aac57d5245848b5c3f45635cdda3 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-0210aac57d5245848b5c3f45635cdda32025-01-03T00:11:46ZengSAGE PublishingMolecular Imaging1536-01212011-11-011010.2310/7290.2011.0001910.2310_7290.2011.00019Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout MiceRaúl HeranceSantiago RojasSergio AbadXavier JiménezJuan Domingo GispertOlga MillánElena Martín-GarcíaAurelijus BurokasMiquel Àngel SerraRafael MaldonadoDeborah ParetoIn this study, we assessed the feasibility of using positron emission tomography (PET) and the tracer [ 11 C]OMAR ([ 11 C]JHU75528), an analogue of rimonabant, to study the brain cannabinoid type 1 (CB1) receptor system. Wild-type (WT) andCB1 knockout (KO) animals were imaged at baseline and after pretreatment with blocking doses of rimonabant. Brain uptake in WT animals was higher (50%) than in KO animals in baseline conditions. After pretreatment with rimonabant, WT uptake lowered to the level of KO animals. The results of this study support the feasibility of using PET with the radiotracer [ 11 C]JHU75528 to image the brain CB1 receptor system in mice. In addition, this methodology can be used to assess the effect of new drugs in preclinical studies using genetically manipulated animals.https://doi.org/10.2310/7290.2011.00019 |
| spellingShingle | Raúl Herance Santiago Rojas Sergio Abad Xavier Jiménez Juan Domingo Gispert Olga Millán Elena Martín-García Aurelijus Burokas Miquel Àngel Serra Rafael Maldonado Deborah Pareto Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice Molecular Imaging |
| title | Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice |
| title_full | Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice |
| title_fullStr | Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice |
| title_full_unstemmed | Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice |
| title_short | Positron Emission Tomographic Imaging of the Cannabinoid Type 1 Receptor System with [C]OMAR ([C]JHU75528): Improvements in Image Quantification Using Wild-Type and Knockout Mice |
| title_sort | positron emission tomographic imaging of the cannabinoid type 1 receptor system with c omar c jhu75528 improvements in image quantification using wild type and knockout mice |
| url | https://doi.org/10.2310/7290.2011.00019 |
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