Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis
It has been suggested that cancer stem cells (CSC) may play a central role in oncogenesis, especially in undifferentiated tumours. Anaplastic thyroid carcinoma (ATC) has characteristics suggestive of a tumour enriched in CSC. Previous studies suggested that the stem cell factor SOX2 has a preeminen...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/439370 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561124691673088 |
|---|---|
| author | Walter Arancio Valeria Carina Giuseppe Pizzolanti Laura Tomasello Maria Pitrone Concetta Baiamonte Marco Calogero Amato Carla Giordano |
| author_facet | Walter Arancio Valeria Carina Giuseppe Pizzolanti Laura Tomasello Maria Pitrone Concetta Baiamonte Marco Calogero Amato Carla Giordano |
| author_sort | Walter Arancio |
| collection | DOAJ |
| description | It has been suggested that cancer stem cells (CSC) may play a central role in oncogenesis, especially in undifferentiated tumours. Anaplastic thyroid carcinoma (ATC) has characteristics suggestive of a tumour enriched in CSC.
Previous studies suggested that the stem cell factor SOX2 has a preeminent hierarchical role in determining the characteristics of stem cells in SW1736 ATC cell line. In detail, silencing SOX2 in SW1736 is able to suppress the expression of the stem markers analysed, strongly sensitizing the line to treatment with chemotherapeutic agents. Therefore, in order to further investigate the role of SOX2 in ATC, a competing endogenous RNA (ceRNA) analysis was conducted in order to isolate new functional partners of SOX2. Among the interactors, of particular interest are genes involved in the biogenesis of miRNAs (DICER1, RNASEN, and EIF2C2), in the control cell cycle (TP53, CCND1), and in mitochondrial activity (COX8A). The data suggest that stemness, microRNA biogenesis and functions, p53 regulatory network, cyclin D1, and cell cycle control, together with mitochondrial activity, might be coregulated. |
| format | Article |
| id | doaj-art-0168b1cac74e43d28ff4058c86853802 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-0168b1cac74e43d28ff4058c868538022025-02-03T01:25:53ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/439370439370Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA BiogenesisWalter Arancio0Valeria Carina1Giuseppe Pizzolanti2Laura Tomasello3Maria Pitrone4Concetta Baiamonte5Marco Calogero Amato6Carla Giordano7Section of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalySection of Cardio-Respiratory and Endocrine-Metabolic Diseases, Biomedical Department of Internal and Specialist Medicine (Di.Bi.M.I.S.), University of Palermo, Piazza delle Cliniche 2, 90127 Palermo, ItalyIt has been suggested that cancer stem cells (CSC) may play a central role in oncogenesis, especially in undifferentiated tumours. Anaplastic thyroid carcinoma (ATC) has characteristics suggestive of a tumour enriched in CSC. Previous studies suggested that the stem cell factor SOX2 has a preeminent hierarchical role in determining the characteristics of stem cells in SW1736 ATC cell line. In detail, silencing SOX2 in SW1736 is able to suppress the expression of the stem markers analysed, strongly sensitizing the line to treatment with chemotherapeutic agents. Therefore, in order to further investigate the role of SOX2 in ATC, a competing endogenous RNA (ceRNA) analysis was conducted in order to isolate new functional partners of SOX2. Among the interactors, of particular interest are genes involved in the biogenesis of miRNAs (DICER1, RNASEN, and EIF2C2), in the control cell cycle (TP53, CCND1), and in mitochondrial activity (COX8A). The data suggest that stemness, microRNA biogenesis and functions, p53 regulatory network, cyclin D1, and cell cycle control, together with mitochondrial activity, might be coregulated.http://dx.doi.org/10.1155/2015/439370 |
| spellingShingle | Walter Arancio Valeria Carina Giuseppe Pizzolanti Laura Tomasello Maria Pitrone Concetta Baiamonte Marco Calogero Amato Carla Giordano Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis International Journal of Endocrinology |
| title | Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis |
| title_full | Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis |
| title_fullStr | Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis |
| title_full_unstemmed | Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis |
| title_short | Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis |
| title_sort | anaplastic thyroid carcinoma a cerna analysis pointed to a crosstalk between sox2 tp53 and microrna biogenesis |
| url | http://dx.doi.org/10.1155/2015/439370 |
| work_keys_str_mv | AT walterarancio anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT valeriacarina anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT giuseppepizzolanti anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT lauratomasello anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT mariapitrone anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT concettabaiamonte anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT marcocalogeroamato anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis AT carlagiordano anaplasticthyroidcarcinomaacernaanalysispointedtoacrosstalkbetweensox2tp53andmicrornabiogenesis |