Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides)
This study investigated the effects of dietary supplementation of mulberry leaf oligosaccharides (MLO) on the growth performance, serum biochemistry, glucose and lipid metabolism, antioxidant activity, liver health, and virus resistance in largemouth bass (Micropterus salmoides). The fish were fed w...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1525992/full |
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| author | Donglai Zhou Donglai Zhou Wenhao Zhong Wenhao Zhong Bing Fu Erna Li Erna Li Le Hao Qingrong Li Qiong Yang Yuxiao Zou Zhenxing Liu Fubao Wang Sentai Liao Dongxu Xing |
| author_facet | Donglai Zhou Donglai Zhou Wenhao Zhong Wenhao Zhong Bing Fu Erna Li Erna Li Le Hao Qingrong Li Qiong Yang Yuxiao Zou Zhenxing Liu Fubao Wang Sentai Liao Dongxu Xing |
| author_sort | Donglai Zhou |
| collection | DOAJ |
| description | This study investigated the effects of dietary supplementation of mulberry leaf oligosaccharides (MLO) on the growth performance, serum biochemistry, glucose and lipid metabolism, antioxidant activity, liver health, and virus resistance in largemouth bass (Micropterus salmoides). The fish were fed with CK (basal diet), MLOL (basal diet supplemented with 0.5%MLO), and MLOH (basal diet supplemented with 1.0% MLO) for 80 days, and then subjected to a 21-day viral challenge experiment. The results showed that MLO supplementation had no adverse effect on the weight gain rate, specific growth rate, feed intake, and condition factor (P > 0.05), but significantly decreased the feed conversion rate and viscerosomatic index (P< 0.05). Moreover, the MLOL and MLOH group had significantly lower contents of triglyceride, blood glucose, and malondialdehyde and activities of serum alanine aminotransferase and aspartate aminotransferase, while significantly higher levels of serum and liver total superoxide dismutase and lower levels of glutathione than the CK group (P< 0.05). MLO supplementation significantly up-regulated the relative expression of glycolytic genes gk and pfk and lipid catabolism genes ppar-α and cpt-1, while obviously down-regulated that of acc, fas, and dgat related to fatty acid synthesis in the liver tissue (P< 0.05). In terms of liver health, MLO supplementation significantly up-regulated the relative expression of anti-inflammatory cytokines il-10 and tgf-β, while decreased that of pro-inflammatory cytokines nf-κb, il-8, and tnf-α in the liver tissue (P< 0.05). The viral challenge test showed that MLO supplementation significantly improved the survival rate of M. salmoides after largemouth bass ranavirus (LMBV) infection. Dietary MLO supplementation promoted liver glucose and lipid metabolism, and improved the immunity and resistance of M. salmoides to LMBV by regulating the PPAR signaling way and inhibiting the NF-kB signaling pathway. The appropriate addition amount of MLO to the diet was determined to be 1.0%. |
| format | Article |
| id | doaj-art-0159a09c0f59400da8e13ef517b900d9 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-0159a09c0f59400da8e13ef517b900d92025-01-28T06:41:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011610.3389/fimmu.2025.15259921525992Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides)Donglai Zhou0Donglai Zhou1Wenhao Zhong2Wenhao Zhong3Bing Fu4Erna Li5Erna Li6Le Hao7Qingrong Li8Qiong Yang9Yuxiao Zou10Zhenxing Liu11Fubao Wang12Sentai Liao13Dongxu Xing14Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaGuangdong Provincial Engineering Technology Research Center of Special Aquatic Functional Feed, Foshan, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaGuangdong Provincial Engineering Technology Research Center of Special Aquatic Functional Feed, Foshan, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Provincial Engineering Technology Research Center of Special Aquatic Functional Feed, Foshan, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, ChinaThis study investigated the effects of dietary supplementation of mulberry leaf oligosaccharides (MLO) on the growth performance, serum biochemistry, glucose and lipid metabolism, antioxidant activity, liver health, and virus resistance in largemouth bass (Micropterus salmoides). The fish were fed with CK (basal diet), MLOL (basal diet supplemented with 0.5%MLO), and MLOH (basal diet supplemented with 1.0% MLO) for 80 days, and then subjected to a 21-day viral challenge experiment. The results showed that MLO supplementation had no adverse effect on the weight gain rate, specific growth rate, feed intake, and condition factor (P > 0.05), but significantly decreased the feed conversion rate and viscerosomatic index (P< 0.05). Moreover, the MLOL and MLOH group had significantly lower contents of triglyceride, blood glucose, and malondialdehyde and activities of serum alanine aminotransferase and aspartate aminotransferase, while significantly higher levels of serum and liver total superoxide dismutase and lower levels of glutathione than the CK group (P< 0.05). MLO supplementation significantly up-regulated the relative expression of glycolytic genes gk and pfk and lipid catabolism genes ppar-α and cpt-1, while obviously down-regulated that of acc, fas, and dgat related to fatty acid synthesis in the liver tissue (P< 0.05). In terms of liver health, MLO supplementation significantly up-regulated the relative expression of anti-inflammatory cytokines il-10 and tgf-β, while decreased that of pro-inflammatory cytokines nf-κb, il-8, and tnf-α in the liver tissue (P< 0.05). The viral challenge test showed that MLO supplementation significantly improved the survival rate of M. salmoides after largemouth bass ranavirus (LMBV) infection. Dietary MLO supplementation promoted liver glucose and lipid metabolism, and improved the immunity and resistance of M. salmoides to LMBV by regulating the PPAR signaling way and inhibiting the NF-kB signaling pathway. The appropriate addition amount of MLO to the diet was determined to be 1.0%.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1525992/fulllargemouth bassgrowthserum biochemistryliver metabolisminflammation |
| spellingShingle | Donglai Zhou Donglai Zhou Wenhao Zhong Wenhao Zhong Bing Fu Erna Li Erna Li Le Hao Qingrong Li Qiong Yang Yuxiao Zou Zhenxing Liu Fubao Wang Sentai Liao Dongxu Xing Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) Frontiers in Immunology largemouth bass growth serum biochemistry liver metabolism inflammation |
| title | Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) |
| title_full | Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) |
| title_fullStr | Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) |
| title_full_unstemmed | Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) |
| title_short | Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides) |
| title_sort | dietary supplementation of mulberry leaf oligosaccharides improves the growth glucose and lipid metabolism immunity and virus resistance in largemouth bass micropterus salmoides |
| topic | largemouth bass growth serum biochemistry liver metabolism inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1525992/full |
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