Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review
Abstract As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of organoids relies mainly on a type of unknown composition scaffold, namely Matrigel, which may pose problems in studying the effect of mechanical properties...
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| Format: | Article |
| Language: | English |
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BMC
2025-03-01
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| Series: | Molecular Medicine |
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| Online Access: | https://doi.org/10.1186/s10020-025-01131-7 |
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| author | Wei Lai Hu Geliang Xu Bin Wei Wang |
| author_facet | Wei Lai Hu Geliang Xu Bin Wei Wang |
| author_sort | Wei Lai |
| collection | DOAJ |
| description | Abstract As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of organoids relies mainly on a type of unknown composition scaffold, namely Matrigel, which may pose problems in studying the effect of mechanical properties on organoids. Hydrogels, a new material with adjustable mechanical properties, can adapt to current studies. In this review, we summarized the synthesis of recent advance in developing definite hydrogel scaffolds for organoid culture and identified the critical parameters for regulating mechanical properties. In addition, classified by different mechanical properties like stiffness and viscoelasticity, we concluded the effect of mechanical properties on the development of organoids and tumor organoids. We hope this review enhances the understanding of the development of organoids by hydrogels and provides more practical approaches to investigating them. |
| format | Article |
| id | doaj-art-0138b9fdc8584ec89cef3d241b013c10 |
| institution | DOAJ |
| issn | 1528-3658 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj-art-0138b9fdc8584ec89cef3d241b013c102025-08-20T02:59:54ZengBMCMolecular Medicine1528-36582025-03-0131111910.1186/s10020-025-01131-7Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive reviewWei Lai0Hu Geliang1Xu Bin2Wei Wang3Department of Thoracic Surgery, Renmin Hospital of Wuhan UniversityDepartment of Orthopedics, Renmin Hospital of Wuhan UniversityCancer Center, Renmin Hospital of Wuhan UniversityDepartment of Thoracic Surgery, Renmin Hospital of Wuhan UniversityAbstract As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of organoids relies mainly on a type of unknown composition scaffold, namely Matrigel, which may pose problems in studying the effect of mechanical properties on organoids. Hydrogels, a new material with adjustable mechanical properties, can adapt to current studies. In this review, we summarized the synthesis of recent advance in developing definite hydrogel scaffolds for organoid culture and identified the critical parameters for regulating mechanical properties. In addition, classified by different mechanical properties like stiffness and viscoelasticity, we concluded the effect of mechanical properties on the development of organoids and tumor organoids. We hope this review enhances the understanding of the development of organoids by hydrogels and provides more practical approaches to investigating them.https://doi.org/10.1186/s10020-025-01131-7OrganoidsStiffnessViscoelasticity3D modelHydrogel |
| spellingShingle | Wei Lai Hu Geliang Xu Bin Wei Wang Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review Molecular Medicine Organoids Stiffness Viscoelasticity 3D model Hydrogel |
| title | Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review |
| title_full | Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review |
| title_fullStr | Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review |
| title_full_unstemmed | Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review |
| title_short | Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review |
| title_sort | effects of hydrogel stiffness and viscoelasticity on organoid culture a comprehensive review |
| topic | Organoids Stiffness Viscoelasticity 3D model Hydrogel |
| url | https://doi.org/10.1186/s10020-025-01131-7 |
| work_keys_str_mv | AT weilai effectsofhydrogelstiffnessandviscoelasticityonorganoidcultureacomprehensivereview AT hugeliang effectsofhydrogelstiffnessandviscoelasticityonorganoidcultureacomprehensivereview AT xubin effectsofhydrogelstiffnessandviscoelasticityonorganoidcultureacomprehensivereview AT weiwang effectsofhydrogelstiffnessandviscoelasticityonorganoidcultureacomprehensivereview |