Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM)
Non-tuberculous mycobacteria (NTM) represent a diverse group of mycobacterial species known for causing opportunistic infections, especially in individuals with underlying health conditions. Unlike <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>), the causative agent of tubercu...
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MDPI AG
2024-11-01
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| Series: | Kinases and Phosphatases |
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| Online Access: | https://www.mdpi.com/2813-3757/2/4/22 |
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| author | Swati Jaiswal Sanjay Kumar |
| author_facet | Swati Jaiswal Sanjay Kumar |
| author_sort | Swati Jaiswal |
| collection | DOAJ |
| description | Non-tuberculous mycobacteria (NTM) represent a diverse group of mycobacterial species known for causing opportunistic infections, especially in individuals with underlying health conditions. Unlike <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>), the causative agent of tuberculosis, NTM species exhibit different pathogenic characteristics and drug resistance mechanisms, making them increasingly relevant in clinical settings. PtkA is a crucial protein tyrosine kinase that regulates bacterial growth, stress response, and virulence by phosphorylating various substrates in <i>Mtb</i>. Understanding whether PtkA homologs exist in NTM could provide insights into their virulence and resistance mechanisms. In silico approaches, which utilize computational tools for sequence alignment, structure prediction, and functional annotation, offer a powerful means to identify homologous proteins across different species. In this article, we have employed tools like BLAST (Basic Local Alignment Search Tool), protein structure databases, and the NTM database to identify PtkA homologs in NTM genomes, providing a foundation for further studies. |
| format | Article |
| id | doaj-art-01374565aaa247d9ac4a64ae5eb3c6b6 |
| institution | Kabale University |
| issn | 2813-3757 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Kinases and Phosphatases |
| spelling | doaj-art-01374565aaa247d9ac4a64ae5eb3c6b62025-01-24T13:37:29ZengMDPI AGKinases and Phosphatases2813-37572024-11-012434034510.3390/kinasesphosphatases2040022Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM)Swati Jaiswal0Sanjay Kumar1Department of Medicine, University of Massachusetts Chan Medical School, Worcester, MA 01605, USADepartment of Structural and Molecular Biology, City College of New York, New York, NY 10031, USANon-tuberculous mycobacteria (NTM) represent a diverse group of mycobacterial species known for causing opportunistic infections, especially in individuals with underlying health conditions. Unlike <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>), the causative agent of tuberculosis, NTM species exhibit different pathogenic characteristics and drug resistance mechanisms, making them increasingly relevant in clinical settings. PtkA is a crucial protein tyrosine kinase that regulates bacterial growth, stress response, and virulence by phosphorylating various substrates in <i>Mtb</i>. Understanding whether PtkA homologs exist in NTM could provide insights into their virulence and resistance mechanisms. In silico approaches, which utilize computational tools for sequence alignment, structure prediction, and functional annotation, offer a powerful means to identify homologous proteins across different species. In this article, we have employed tools like BLAST (Basic Local Alignment Search Tool), protein structure databases, and the NTM database to identify PtkA homologs in NTM genomes, providing a foundation for further studies.https://www.mdpi.com/2813-3757/2/4/22PtkA<i>Mycobacterium tuberculosis</i>nontuberculous bacteriatyrosine kinase |
| spellingShingle | Swati Jaiswal Sanjay Kumar Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) Kinases and Phosphatases PtkA <i>Mycobacterium tuberculosis</i> nontuberculous bacteria tyrosine kinase |
| title | Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) |
| title_full | Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) |
| title_fullStr | Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) |
| title_full_unstemmed | Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) |
| title_short | Using In Silico Methods to Identify Protein Tyrosine Kinase A (PtkA) Homolog in Non-Tuberculous Mycobacteria (NTM) |
| title_sort | using in silico methods to identify protein tyrosine kinase a ptka homolog in non tuberculous mycobacteria ntm |
| topic | PtkA <i>Mycobacterium tuberculosis</i> nontuberculous bacteria tyrosine kinase |
| url | https://www.mdpi.com/2813-3757/2/4/22 |
| work_keys_str_mv | AT swatijaiswal usinginsilicomethodstoidentifyproteintyrosinekinaseaptkahomologinnontuberculousmycobacteriantm AT sanjaykumar usinginsilicomethodstoidentifyproteintyrosinekinaseaptkahomologinnontuberculousmycobacteriantm |