Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study
Mesenchymal stem cell (MSC) therapy shows considerable promise for the treatment of myocardial infarction (MI). However, the inefficient migration and homing of MSCs after systemic infusion have limited their therapeutic applications. Ultrasound-targeted...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/691310 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562783311364096 |
|---|---|
| author | Lu Li Shengzheng Wu Zheng Liu Zhongxiong Zhuo Kaibin Tan Hongmei Xia Lisha Zhuo Xiaojun Deng Yunhua Gao Yali Xu |
| author_facet | Lu Li Shengzheng Wu Zheng Liu Zhongxiong Zhuo Kaibin Tan Hongmei Xia Lisha Zhuo Xiaojun Deng Yunhua Gao Yali Xu |
| author_sort | Lu Li |
| collection | DOAJ |
| description | Mesenchymal stem cell (MSC) therapy shows considerable promise for the
treatment of myocardial infarction (MI). However, the inefficient migration and homing of
MSCs after systemic infusion have limited their therapeutic applications. Ultrasound-targeted
microbubble destruction (UTMD) has proven to be promising to improve the homing of MSCs
to the ischemic myocardium, but the concrete mechanism remains unclear. We hypothesize
that UTMD promotes MSC homing by upregulating SDF-1/CXCR4, and this study was aimed
at exploring this potential mechanism. We analyzed SDF-1/CXCR4 expression after UTMD
treatment in vitro and in vivo and counted the number of homing MSCs in MI areas. The
in vitro results demonstrated that UTMD not only led to elevated secretion of SDF-1
but also resulted in an increased proportion of MSCs that expressed surface CXCR4.
The in vivo findings show an increase in the number of homing MSCs and higher expression
of SDF-1/CXCR4 in the UTMD combined with MSCs infusion group compared to other groups.
In conclusion, UTMD can increase SDF-1 expression in the ischemic myocardium and upregulate
the expression of surface CXCR4 on MSCs, which provides a molecular mechanism for the homing
of MSCs assisted by UTMD via SDF-1/CXCR4 axis. |
| format | Article |
| id | doaj-art-00909db6596c4a9cb49f3d9682bf32c9 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-00909db6596c4a9cb49f3d9682bf32c92025-02-03T01:21:46ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/691310691310Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot StudyLu Li0Shengzheng Wu1Zheng Liu2Zhongxiong Zhuo3Kaibin Tan4Hongmei Xia5Lisha Zhuo6Xiaojun Deng7Yunhua Gao8Yali Xu9Department of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Blood Transfusion, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, ChinaMesenchymal stem cell (MSC) therapy shows considerable promise for the treatment of myocardial infarction (MI). However, the inefficient migration and homing of MSCs after systemic infusion have limited their therapeutic applications. Ultrasound-targeted microbubble destruction (UTMD) has proven to be promising to improve the homing of MSCs to the ischemic myocardium, but the concrete mechanism remains unclear. We hypothesize that UTMD promotes MSC homing by upregulating SDF-1/CXCR4, and this study was aimed at exploring this potential mechanism. We analyzed SDF-1/CXCR4 expression after UTMD treatment in vitro and in vivo and counted the number of homing MSCs in MI areas. The in vitro results demonstrated that UTMD not only led to elevated secretion of SDF-1 but also resulted in an increased proportion of MSCs that expressed surface CXCR4. The in vivo findings show an increase in the number of homing MSCs and higher expression of SDF-1/CXCR4 in the UTMD combined with MSCs infusion group compared to other groups. In conclusion, UTMD can increase SDF-1 expression in the ischemic myocardium and upregulate the expression of surface CXCR4 on MSCs, which provides a molecular mechanism for the homing of MSCs assisted by UTMD via SDF-1/CXCR4 axis.http://dx.doi.org/10.1155/2015/691310 |
| spellingShingle | Lu Li Shengzheng Wu Zheng Liu Zhongxiong Zhuo Kaibin Tan Hongmei Xia Lisha Zhuo Xiaojun Deng Yunhua Gao Yali Xu Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study Stem Cells International |
| title | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study |
| title_full | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study |
| title_fullStr | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study |
| title_full_unstemmed | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study |
| title_short | Ultrasound-Targeted Microbubble Destruction Improves the Migration and Homing of Mesenchymal Stem Cells after Myocardial Infarction by Upregulating SDF-1/CXCR4: A Pilot Study |
| title_sort | ultrasound targeted microbubble destruction improves the migration and homing of mesenchymal stem cells after myocardial infarction by upregulating sdf 1 cxcr4 a pilot study |
| url | http://dx.doi.org/10.1155/2015/691310 |
| work_keys_str_mv | AT luli ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT shengzhengwu ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT zhengliu ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT zhongxiongzhuo ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT kaibintan ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT hongmeixia ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT lishazhuo ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT xiaojundeng ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT yunhuagao ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy AT yalixu ultrasoundtargetedmicrobubbledestructionimprovesthemigrationandhomingofmesenchymalstemcellsaftermyocardialinfarctionbyupregulatingsdf1cxcr4apilotstudy |