Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR

Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR

The increase in emerging and reemerging infectious diseases has underscored the need for the prompt monitoring of intact infectious viruses and the quick assessment of their infectivity. However, molecular techniques cannot distinguish between intact infectious and noninfectious viruses. Here, two d...

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Bibliographic Details
Main Authors: Chudan Liang, Zequn Wang, Linjin Fan, Yulong Wang, Yuandong Zhou, Xiaofeng Yang, Jingyan Lin, Pengfei Ye, Wendi Shi, Hongxin Huang, Huijun Yan, Linna Liu, Jun Qian
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Biosafety and Health
Subjects:
Intact infectious virus
Rapid quantification
Integrated cell absorption quantitative polymerase chain reaction (ICA-qPCR)
Propidium monoazide quantitative polymerase chain reaction (PMA-qPCR)
Inactivation
Online Access:http://www.sciencedirect.com/science/article/pii/S2590053624001356
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