MiR-223 within neutrophil axis promotes Th17 expansion by PI3K-AKT pathway in systemic lupus erythematosus by Chengzhong Zhang, Yan Lu

“…We assessed miR-223 expression within neutrophils and Th17 expansion in MRL/lpr mice and patients with SLE using RT-PCR, luciferase reporter assay, Elisa, flow cytometry analysis. Signaling pathway, RT-PCR and western blot were conducted to elucidate the mechanism by which miR-223 within neutrophils expands Th17. …”
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Studies of heavy metal contents and microbial composition of rhizosphere of <i>Panicum maximum</i> within and around auto mechanic workshop in Benin City by O Osazuwa, I Beckley, UC Osayamen

“…It was then taken to the laboratory for heavy metals and microbial assay. The results showed that average plant density of Panicum maximum per 100m2 of sampled subplots in the mechanic workshops was 8.50 in Ikpoba-Okha and 9.00 in Oredo, compared to 27.50 in the control. …”
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Endoscopic Biopsy in Differential Diagnosis of Colorectal Serrated Lesions by K. D. Khalin, M. Yu. Agapov, L. V. Zvereva, K. V. Stegniy

“…Assessment of sensitivity, specificity and accuracy of endoscopic biopsy in differential diagnosis of colorectal serrated adenomas and risk factors for variance between biopsy results and morphological examination of completely resected lesions.Materials and methods. The assay used data on 56 morphologically verified serrated adenomas diagnosed and resected in 50 patients (14 men, 36 women; average age 66.9 ± 10.5 years). …”
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Assessment of cytotoxicity of clear aligners coated with zinc oxide nanoparticles by Indu Ravi, Vignesh Kailasam

“…The cytotoxicity on HGFs was assessed at day 0, 7 and 14 using assay ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]). …”
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Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer. by Pierre Alusta, Angel Paredes, Marli Azevedo, Lisa Mullis, Dan Buzatu

“…Since respiratory viruses are typically present in nasal and oral secretions, saliva is a good target for testing for viral infections. …”
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Effects of Endurance Training and Chlorogenic Acid on Oxidative and Nitrosative Stress Markers in Prediabetic Male Mice by Sahar Ghasemi Pour, Sayyed M. Marandi

“…Next, 24 hours after the last intervention, tissue samples were taken from the quadriceps femoris muscles and analyzed using the enzyme-linked immunosorbent assay (ELISA) method. Data were analyzed using one-way analysis of variance and Dunnett’s post-hoc test at a significance level of P < 0.05. …”
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Antioxidant activity of Micractinium sp. (Chlorophyta) extracts against H2O2 induced oxidative stress in human breast adenocarcinoma cells by Onur Bulut, Işkın Engin Köse, Çağla Sönmez, Hüseyin Avni Öktem

“…The methanolic extracts demonstrated the highest antioxidant capacity, measuring 7.72 and 93.80 µmol trolox equivalents g−1 dry weight (DW) according to the DPPH and FRAP assays, respectively. To further characterize the biochemical profile, reverse phase high-performance chromatography (RP-HPLC) was employed to quantify twelve different phenolics, including rutin, gallic acid, benzoic acid, cinnamic acid, and β-carotene, in the microalgal extracts. …”
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Effect of Bio MTA plus & ProRoot MTA pulp capping materials on the regenerative properties of human dental pulp stem cells by Khaled Mohamed Ayoub, Mohamed Mokhtar Nagy, Riham Mohamed Aly, Ghada Nour El Deen, Karim El-Batouty

“…The effects of the extracts on hDPSCs cytotoxicity and proliferation were assessed via a CCK-8 assay. Annexin V expression was investigated to assess the effects of both materials on the induction of apoptosis. …”
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Deciphering the generation of heterogeneity in esophageal squamous cell carcinoma metastasis via single-cell multiomics analysis by Kaiwen Sheng, Jun Chen, Ruitang Xu, Haoqiang Sun, Ran Liu, Yongjie Wang, Wenwen Xu, Jiao Guo, Miao Zhang, Shuai Liu, Juan Lei, Yawen Sun, Yang Jia, Dianhao Guo

“…Methods We performed single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) on cancerous and adjacent noncancerous tissues from four ESCC patients who were pathological staged as T1a, T2b, T3b, or T4a, to investigate whether regulatory elements are pivotal in instigating cellular heterogeneity during ESCC metastasis. …”
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Protein tyrosine phosphatase delta is a STAT3-phosphatase and suppressor of metabolic liver disease by Thomas F Baumert, Charlotte Bach, Patrick Pessaux, Catherine Schuster, Frank Jühling, Sarah C Durand, Yujin Hoshida, Fabien Zoulim, Atish Mukherji, Naoto Fujiwara, Marine A Oudot, Armando Andres Roca Suarez, Michel L Tremblay, Barbara Testoni, Joachim Lupberger, Romain Parent, Laurent Mailly, Nassim Dali-Youcef, Emanuele Felli, Maria Saez-Palma, Julien Moehlin, Alessia Virzì, Nicolas Brignon, Eugenie Schaeffer, Romain Martin, Laura Meiss-Heydmann, Zakaria Boulahtouf, Lea Girard, Emma Osswald, Carole Jamey, Daniel Brumaru, Bhuvaneswari Koneru

“…Identified pathways were validated by perturbation studies in human hepatocytes and PTPRD substrates by pull-down assays. The clinical relevance was further explored in a cohort with metabolic disease by ranking patients according to PTPRD expression and analysing its association with metabolic disease markers.Results The analysis of individuals ranked according to PTPRD expression and Ptprd-deficient mice, showed that PTPRD levels were associated with hepatic glucose/lipid signalling and peroxisome function. …”
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Multimodal treatment with Neoadjuvant chemotherapy for eyelid and periocular sebaceous gland carcinoma: Long-term outcomes by Vijitha S. Vempuluru, Anshika Luthra, Vijay Anand Reddy Palkonda, Swathi Kaliki

“…The lower eyelid was the tumor epicenter in two (66 %) patients and the medial canthus in one (33 %) patient. The mean tumor basal diameter was 27 mm (median, 30 mm, range, 20–30 mm). …”
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A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products by Wen Cui, Liang Zhao, Yuwen Shi, Wei Feng, Xiao Chen, Hui Liu

“…The reliability and accuracy of microbiological assay heavily relies on this step. Currently in China, the most recognized standard method for such medium evaluation is ISO 11133-2014. …”
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Acute and chronic effects of physical exercise on atherosclerosis, kynurenine pathway, endothelial function and inflammation in patients with coronary artery disease: a clinical tr... by Eliete Bouskela, Gabriela Andrade Paz, Marcus VDS Rangel, Caio Luan Farias, André Luiz Conveniente Soares, Erica Langner, Tatiana Teixeira, Patrícia M L Dutra, Paulo Farinatti, Maria das Graças C Souza, Juliana P Borges

“…Levels of KP metabolites (TRP, KYN, kynurenic acid and quinolinic acid), inflammatory cytokines (interleukin-6, tumour necrosis factor-alpha and interferon-gamma), T-cell subpopulations and soluble adhesion molecules (soluble P-selectin, soluble vascular cell adhesion molecule-1 and soluble intercellular adhesion molecule-1) will be assessed via ELISA, flow cytometry and multiplex assays, respectively. This study was approved by the Ethics Committee of Pedro Ernesto University Hospital (CAAE 81718324.3.0000.5259) and registered on ClinicalTrials.gov (NCT06579807). …”
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Redistribuição, reconhecimento e poder no financiamento público do cinema pernambucano: uma análise longitudinal da política pública de cultura do estado no âmbito das desigualdade... by Bárbara Cristina do Santos Lino

“… No Recife, observou-se uma vasta produção cinematográfica, apoiada financeiramente por uma política pública (Lei Estadual 12.310/2002), com expressivo foco em temáticas urbanas, desdobrando-se no engajamento pelo direito à cidade. …”
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Competitive displacement of lipoprotein lipase from heparan sulfate is orchestrated by a disordered acidic cluster in GPIHBP1 by Anamika Biswas, Samina Arshid, Kristian Kølby Kristensen, Thomas J.D. Jørgensen, Michael Ploug

“…Modeling this interface revealed widespread polyelectrolyte interactions spanning both LPL domains, which explains why the acidic tail stabilizes LPL activity and protein conformation. In functional assays, we showed that the acidic cluster at 19–30 also had the greatest impact on preserving LPL activity, mitigating ANGPTL4-catalyzed LPL inactivation, preventing PSCK3-mediated LPL cleavage, and, importantly, displacing LPL from HSPGs. …”
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A comparative study of chemical composition, phenolic compound profile and antioxidant activity of wild grown, field and greenhouse cultivated Physalis (P. alkekengi and P. peruvia... by Parisa Yari, Abolfazl Alirezalu, Soghra Khalili

“…The uppermost antioxidant activity was obtained in the fruit extract of cultivated P.alkekengi in C5 (Silvana) with 44.13% by DPPH method and C8 (Urmia) with 0.40 μM Fe++ g −1 FW by FRAP assay. Extracts obtained C8 and C4 (Khoy) possessed the highest total phenolic (34.12 mg GAE g−1 FW) and total flavonoid (7.06 mg QUE g−1 FW) contents, respectively. …”
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Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli. by Danelle S Eto, Tiffani A Jones, Jamie L Sundsbak, Matthew A Mulvey

“…Several host proteins have previously been identified as receptors for type 1 pili, but none have been conclusively shown to promote UPEC entry into host bladder cells. Using overlay assays with FimH, the purified type 1 pilus adhesin, and mass spectroscopy, we have identified beta1 and alpha3 integrins as key host receptors for UPEC. …”
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Site-selective dimethylation of flavonoids using fusion flavonoid O-methyltransferases by Kyungha Lee, Seong Hee Bhoo, Sang-Won Lee, Man-Ho Cho

“…The fusion FOMTs (OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10) were heterologously expressed in Escherichia coli. Activity assays of the recombinant fusion FOMTs demonstrated that OsNOMT/ObFOMT5, OsNOMT/RdOMT10, and ObFOMT5/RdOMT10 catalyze 7/4'-O-methylations, 7/3-O-methylations, and 4'/3-O-methylations of flavonoids, respectively. …”
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Proteomics unveil candidate biomarkers and pathogenesis of subacute thyroiditis by Litong Ran, Xiufei Liu, Yongfeng Tian, Jiaran Zhu, Zhengyuan Gong, Qiao Qiao, Xin Jiang, Yuren Wang, Guojun Yang, Hongting Zheng, Yi Zheng, Hua Qu

“…Moreover, the top three high-abundance proteins (nicotinamide N-methyltransferase (NNMT), FTL and thymidine phosphorylase (TYMP)) were further validated in the plasma from a larger SAT cohort using an enzyme-linked immunosorbent assay. After adjusting for sex, Spearman correlation analysis showed that NNMT, FTL and TYMP levels were positively correlated with FT3, FT4, T3, T4, Tg and erythrocyte sedimentation rate and negatively correlated with thyroid-stimulating hormone. …”
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Attentional dysfunction arises from right frontocentral and occipital network connectivity in Parkinson's disease by Isobel Timothea French, Kuo-Hsuan Chang, Wei-Kuang Liang, Wen-Sheng Chang, Yen-Shi Lo, Yi-Ru Wang, Mei-Ling Cheng, Norden E. Huang, Hsiu-Chuan Wu, Siew-Na Lim, Chiung-Mei Chen, Chi-Hung Juan

“…In Parkinson's disease (PD), the attentional network is impaired due to dysfunctional fronto-subcortical circuits connected to the basal ganglia, disrupting response selection and inhibition. …”
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