Cystatin B Promotes the Proliferation, Migration, and Invasion of Intrahepatic Cholangiocarcinoma by Dai Zhang, Bao-Ye Sun, Jing-Fang Wu, Zhu-Tao Wang, Su-Su Zheng, Guo-Qiang Sun, Xu-Kang Gao, Jian Zhou, Jia Fan, Bo Hu, Shuang-Jian Qiu, Bo-Heng Zhang

“…<b>Conclusions</b>: CSTB plays a significant function in the regulation of iCCA progression and may serve as a promising biomarker for iCCA.…”
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CRISPR/dCas-mediated counter-silencing: reprogramming dCas proteins into antagonists of xenogeneic silencers by Johanna Wiechert, Biel Badia Roigé, Doris Dohmen-Olma, Hindra, Xiafei Zhang, Roberto G. Stella, Marie A. Elliot, Julia Frunzke

“…The strongest effect was observed when targeting the Lsr2 nucleation site. Genome-wide transcriptome profiling revealed high specificity with minimal off-target effects. …”
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A Zeolitic Imidazolate Framework-Based Antimicrobial Peptide Delivery System with Enhanced Anticancer Activity and Low Systemic Toxicity by Jingwen Jiang, Kaderya Kaysar, Yanzhu Pan, Lijie Xia, Jinyao Li

“…We characterized this system in terms of morphology, particle size, zeta potential, infrared (IR), x-ray diffraction (XRD), and transcriptome analysis, and examined the in vitro cytotoxicity and cellular uptake properties of CEC@ZIF-8 using cervical cancer cells. …”
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Characterization and immunoprotective efficacy of a fumarate reductase frdA mutant of Salmonella enteritidis by Siping Zhu, Xinyi Sun, Xinyi Sun, Hong Li, Yongmei Su, Chihuan Li, Xintong Zhu, Chao Ren, Xiaochen Liu, Yulai Dong, Qiumei Shi, Zhiqiang Zhang

“…Moreover, immunization of mice with the frdA deletion strain provided promising immune protection for mice.ConclusionFumarate reductase FrdA is closely associated with pathogenicity of S. enteritidis and that is an attractive candidate target for vaccine design of Salmonella.…”
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Gut microbiota-derived indoleacetic acid attenuates neuroinflammation and neurodegeneration in glaucoma through ahr/rage pathway by Ning Wang, Chengyang Sun, Yijie Yang, Dandan Zhang, Lulu Huang, Chenrui Xu, Minghan Wang, Mengmeng Xu, Tongtong Yan, Yue Wu, Li Xu, Yahan Ju, Hao Sun, Wenyi Guo

“…To explore the underlying mechanisms, retinal transcriptomics and microglia-neuron co-cultures were also employed. …”
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Repurposed clindamycin suppresses pyroptosis in tumor-associated macrophages through Inhibition of caspase-1 by Adrian Weich, Johannes Berges, Cindy Flamann, Katrin Bitterer, Krishna Pal Singh, David Chambers, Christopher Lischer, Xin Lai, Olaf Wolkenhauer, Carola Berking, Gerhard Krönke, Shailendra Gupta, Heiko Bruns, Julio Vera, Research Group Macrophages

“…The method generates a ranking of drug-target interactions, the most promising of which are channeled towards experimental validation. …”
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Molecular markers of reduced behavioral sensitivity to transfluthrin in Anopheles gambiae s.s. from Western Kenya by Stephen Okeyo, Dieunel Derilus, Lucy Mackenzie Impoinvil, Nsa Dada, Diana Omoke, Helga Saizonou, Cynthia Awuor Odhiambo, Nicola Mulder, Gerald Juma, Benard W. Kulohoma, John E. Gimnig, Luc S. Djogbénou, Audrey Lenhart, Eric Ochomo

“…The use of spatial repellent products (SR) containing volatile insecticides such as transfluthrin offer a promising complementary strategy to current vector control tools. …”
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A high content clonogenic survival drug screening identifies maytansine as a potent radiosensitizer for meningiomas by Jinxiu Yu, Jinxiu Yu, Jinxiu Yu, Jiaojiao Deng, Jiaojiao Deng, Jiaojiao Deng, Leihao Ren, Leihao Ren, Leihao Ren, Lingyang Hua, Lingyang Hua, Lingyang Hua, Tianqi Wu, Yi Hui, Chunlin Shao, Ye Gong, Ye Gong, Ye Gong, Ye Gong

“…These findings opened up a promising path in the development of radiosensitizers aimed at treating this condition.…”
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NLRX1 limits inflammatory neurodegeneration in the anterior visual pathway by Alexander J. Gill, Matthew D. Smith, Danny Galleguillos, Thomas Garton, Jackson W. Mace, Sachin P. Gadani, Swati Kumar, Aayush Pokharel, Krista Solem, Saahith Potluri, Omar Hussein, Giuliana Sardi Rogines, Arihant Singh, Annatje Clark, Peter A. Calabresi, Marjan Gharagozloo

“…This study highlights that NLRX1 could serve as a promising target for neuroprotection in progressive MS and other neurodegenerative diseases. …”
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LINC01088 prevents ferroptosis in glioblastoma by enhancing SLC7A11 via HLTF/USP7 axis by Yujie Zhou, Zhen Zhao, Cheng Jiang, Chuansheng Nie, Dongdong Xiao, Zhipeng Wu, Hao Yu, Jianglin Zheng, Xuan Wang, Xiaobing Jiang

“…Ferroptosis, an iron‐dependent cell death pathway, is a promising therapeutic target for GBM. However, current treatments targeting cell death pathways have not yielded expected results. …”
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Decreased IL-33 in the brain following repetitive mild traumatic brain injury contributes to cognitive impairment by inhibiting microglial phagocytosis by Ze-Xi Jia, Meng-Tian Guo, Mei-Mei Li, Pan Liao, Bo Yan, Wei Zhang, Fang-Yuan Cheng, Ya-Ru Liu, Zi-Han Zhang, Cheng Wei, Jie Zhou, Fang-Lian Chen, Ping Lei, Xin-Tong Ge

“…In the mouse brain, astrocytes were identified as the primary source of IL-33 secretion, while microglia predominantly expressed ST2. Transcriptome sequencing revealed that IL-33 significantly influenced phagocytosis function. …”
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Putative function and prognostic molecular marker of mast cells in colorectal cancer by Jiani Guo, Jie Chen, Yiting Wang, Xiaoming Bai, Haimei Feng, Siqi Sheng, Hongyu Wang, Ke Xu, Mengxi Huang, Zengjie Lei, Xiaoyuan Chu

“…Methods Single-cell transcriptome data of CRC patients were extracted from GSE146771 for cell classification and annotation. …”
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PRMT5 deficiency in myeloid cells reprograms macrophages to enhance antitumor immunity and synergizes with anti-PD-L1 therapy by Jian Cao, Yongyu Chen, Bingqian Zhou, Shiyu Chen, Zheyi Chen, Yiren Huang, Lisong Shen, Yingxia Zheng

“…Bulk and single-cell transcriptomics were employed to explore the mechanistic roles of PRMT5 in regulating lipid metabolism and macrophage polarization. …”
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E2F1/CDK5/DRP1 axis mediates microglial mitochondrial division and autophagy in the pathogenesis of cerebral ischemia‐reperfusion injury by Ya‐Jing Yuan, Tingting Chen, Yan‐Ling Yang, Hao‐Nan Han, Li‐Ming Xu

“…Methods A CIRI mouse model was utilized for single‐cell RNA transcriptome sequencing of brain tissues. The research comprised diverse gene expression, gene ontology (GO), and the enrichment of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. …”
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Self-assembled co-delivery nanoplatform for increasing the broad-spectrum susceptibility of fall armyworm toward insecticides by Shuo Yan, Mingjian Li, Qinhong Jiang, Mingshan Li, Mengfan Hu, Xueyan Shi, Pei Liang, Meizhen Yin, Xiwu Gao, Jie Shen, Lei Zhang

“…Method: Regulatory function of nuclear factor erythroid 2-related factor 2 (Nrf2) in detoxification was confirmed using transcriptome sequencing, quantitative real-time PCR and enzyme activity measurement. …”
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Characterizing transcripts of HIV-1 different substrains using direct RNA sequencing by Weizhen Li, Yong Huang, Haowen Yuan, Jingwan Han, Zhengyang Li, Aiping Tong, Yating Li, Hanping Li, Yongjian Liu, Lei Jia, Xiaolin Wang, Jingyun Li, Bohan Zhang, Lin Li

“…This study provides a detailed analysis of post-transcriptional processing modifications in HIV-1 and suggests promising avenues for future research that could potentially be applied as new therapeutic targets in HIV treatment.…”
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A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway by Huan-Huan Zhou, Xiaolei Zhou, Jianqiu Pei, Shiyin Xu, Biyu Jin, Jiuling Chen, Zixuan Zhang, Mingmeng Tang, Yan Liu, Andreas K. Nüssler, Liegang Liu, Qin Xu, Anxin Wang, Min Xia, Wei Yang

“…This study is the first to highlight the multifaceted therapeutic effects of GqDNVs-gel, offering a promising strategy for enhancing cardiac function post-MI. …”
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Identification and Mechanistic Studies of Key Genes in Thalamic Hemorrhage Pain by Multi-omics by Chen Yang, Ju Gao, Yaqun Li, Yinggang Xiao, Tianfeng Huang

“…These findings provide novel mechanistic insights into THP pathogenesis and highlight promising targets for therapeutic intervention.…”
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LINC02888 promotes HGSOC progression and immune evasion via PPIB-mediated stabilization of LAPTM5 mRNA and inhibition of RIG-I-like receptor signaling by BingJie Rui, YanFeng Yang, DanBo Geng, ZiTeng Kuang, TianLi Mu, YuXi Liu, Bo Ren, RunZe He, XiaoWei Zhang, YuCi Zhang, Min Wang

“…Methods We identified overexpression of LINC02888 in HGSOC tissues and cell lines through whole-transcriptome sequencing, qPCR, in situ hybridization, and analyses of TCGA/GTEx datasets. …”
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Dual Roles of SIRT7 Inhibition by Oroxylin A Reprogram HSCs Fate: PRMT5 Succinylation-Driven Senescence and Ecto-Calreticulin-Dependent NK Cell Immune Clearance in Liver Fibrosis by Junrui Wang, Haoyuan Tian, Yuanyuan Gao, Xinran Qiu, Zhengyang Bao, Danli Zhao, Feng Zhang, Zili Zhang, Feixia Wang, Shizhong Zheng, Haibo Cheng, Jiangjuan Shao

“…Activation of hepatic stellate cells (HSCs) represents a central pathological event in liver fibrogenesis, and targeted clearance of activated HSCs is considered to be a promising therapeutic strategy. However, our understanding of the underlying molecular mechanisms is limited. …”
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