Surface Display of Type 1 Fimbriae on <i>Shigella flexneri</i> Induces Antigen-Specific Immune Response via Oral Route by Shuli Sang, Rui Yu, Yunyun Mao, Yanfang Zhai, Chen Cao, Kai Li, Yiyan Guan, Haoxia Tao, Chunjie Liu, Yanchun Wang

“…Background: Live attenuated bacteria are promising candidates for mucosal vaccine delivery due to their ability to elicit robust immune responses. …”
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Genome-Wide Identification and Expression Profiling of Glycosidases, Lipases, and Proteases from Invasive Asian Palm Weevil, <i>Rhynchophorus ferrugineus</i> by Nazmi Harith-Fadzilah, Mohammad Nihad, Mohammed Ali AlSaleh, Abdulqader Yaslam Bazeyad, Subash-Babu Pandurangan, Kashif Munawar, Arya Vidyawan, Hattan A. Alharbi, Jernej Jakše, Arnab Pain, Binu Antony

“…Digestive enzymes (DEs), which impair feeding on plants (herbivory), are promising targets. We generated de novo transcriptomes, annotated DE-related genes from the <i>R. ferrugineus</i> gut and abdomen, manually annotated the DE gene family from the recently available genome and our transcriptome data, and reported 34 glycosidases, 85 lipases, and 201 proteases. …”
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Multi-tissue expression and splicing data prioritise anatomical subsite- and sex-specific colorectal cancer susceptibility genes by Emma Hazelwood, Daffodil M. Canson, Benedita Deslandes, Xuemin Wang, Pik Fang Kho, Danny Legge, Andrei-Emil Constantinescu, Matthew A. Lee, D. Timothy Bishop, Andrew T. Chan, Stephen B. Gruber, Jochen Hampe, Loic Le Marchand, Michael O. Woods, Rish K. Pai, Stephanie L. Schmit, Jane C. Figueiredo, Wei Zheng, Jeroen R. Huyghe, Neil Murphy, Marc J. Gunter, Tom G. Richardson, Vicki L. J. Whitehall, Emma E. Vincent, Dylan M. Glubb, Tracy A. O’Mara

“…These findings offer valuable insights into CRC molecular mechanisms and support promising therapeutic avenues.…”
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MⅡ-polyphosphate-based wax-like material with osteogenesis and blood occlusion by Wei Zhu, Huasi Zhou, Zhanqi Wei, Xisheng Weng, Bin Li, Song Chen, Håkan Engqvist, Sheng Cui, Wei Xia

“…Overall, CoPPW exhibits excellent hemostatic and osteogenic effects, making it a promising candidate for bone repair applications.…”
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Wilms tumor primary cultures capture phenotypic heterogeneity and facilitate preclinical screening by Lisa Götz, Jenny Wegert, Alireza Paikari, Silke Appenzeller, Sabrina Bausenwein, Christian Vokuhl, Taryn D. Treger, Jarno Drost, Christin Linderkamp, Dominik T. Schneider, Karen Ernestus, Steven W. Warman, Jörg Fuchs, Nils Welter, Norbert Graf, Sam Behjati, Rhoikos Furtwängler, Manfred Gessler

“…Adherent, spheroid and organoid cultures are also clearly distinguishable by their transcriptome. Preclinical drug screening experiments revealed sensitivity to a range of inhibitors, that are highly effective in other childhood solid tumors. …”
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Development of MDM2‐Targeting PROTAC for Advancing Bone Regeneration by Sol Jeong, Jae‐Kook Cha, Wasim Ahmed, Jaewan Kim, Minsup Kim, Kyung Tae Hong, Wonji Choi, Sunjoo Choi, Tae Hyeon Yoo, Hyun‑Ju An, Seung Chan An, Jaemin Lee, Jimin Choi, Sun‐Young Kim, Jun‐Seok Lee, Soonchul Lee, Junwon Choi, Jin Man Kim

“…Next, this work performs whole‐transcriptome analysis to dissect the biological effects of the CL144, and reveals the upregulation of osteogenic marker genes. …”
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Ethyl β-carboline-3-carboxylate targets PRDX5/c-Jun axis for novel therapeutic strategy against cervical cancer by Wan-Qiu Xiao, Dong-Hun Lee, Hakjin Kim, Hui-Na Zhang, Yao-Yuan Feng, Ying-Hua Jin, Mei-Hua Jin, Ying-Hao Han, Taeho Kwon, Hu-Nan Sun

“…Our study provides compelling evidence for the development of PRDX5-targeted therapies using β-CCE as a promising therapeutic agent for cervical cancer treatment.…”
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Evolution of agricultural biotechnology is the paradigm shift in crop resilience and development: a review by Muhammad Riaz, Erum Yasmeen, Bilal Saleem, Muhammad Khalid Hameed, Maryam Thani Saeed Almheiri, Reem Omar Saeed Al Mir, Ghalia Alameri, Jwaher Salem Khamis Alghafri, Mayank Anand Gururani

“…The integration of cutting-edge multi-omics platforms, including transcriptomics, proteomics, metabolomics combined with robust artificial intelligence (AI) based methods has revolutionizing crop genome elucidation. …”
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Identification and validation of HOXC6 as a diagnostic biomarker for Ewing sarcoma: insights from machine learning algorithms and in vitro experiments by Yonghua Pang, Jiahui Liang, Jiahui Liang, Yakai Deng, Weinan Chen, Yunyan Shen, Jing Li, Xin Wang, Zhiyao Ren

“…Functional validation through HOXC6 knockdown in the RD-ES cell line revealed that its suppression significantly inhibited cell proliferation and migration. Furthermore, transcriptome sequencing suggested potential oncogenic mechanisms underlying HOXC6 function.DiscussionThese findings highlight HOXC6 as a promising diagnostic biomarker for ES, demonstrating robust performance across multiple datasets. …”
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Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning by Tao Zhou, Can Zhu, Wei Zhang, Qiongfang Wu, Mingqiang Deng, Zhiwei Jiang, Longfei Peng, Hao Geng, Zhouting Tuo, Zhouting Tuo, Ci Zou

“…Among them, PLAC8 is a promising diagnostic biomarker that modulates the immune response in patients with IC/BPS, which provides new insights into the future diagnosis of IC/BPS.…”
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Gut microbiota dysbiosis orchestrates vitiligo-related oxidative stress through the metabolite hippuric acid by Qingrong Ni, Lin Xia, Ye Huang, Xiaoying Yuan, Weijie Gu, Yueqi Chen, Yijin Wang, Meng Nian, Shengxi Wu, Hong Cai, Jing Huang

“…However, the complex promising interaction between abnormal accumulation of reactive oxygen species (ROS) in the skin and gut microbiota has remained unclear. …”
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ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma by Yuhao Pan, Hao Chen, Chenhui Lv, Wei He, Yongpeng Xu, Qijia Xuan

“…Conclusion ATP6V1C1 multifacetedly contributes to the oncogenesis and progression of HCC and is a promising diagnostic and prognostic biomarker with predictive value on therapy response.…”
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Malignant Hepatoblast‐Like Cells Sustain Stemness via IGF2‐Dependent Cholesterol Accumulation in Hepatoblastoma by Miao Ding, Siwei Mao, Han Wu, Sijia Fang, Ni Zhen, Tianshu Chen, Jiabei Zhu, Xiaochen Tang, Xiaoyang Wang, Fenyong Sun, Guoqing Zhu, Qiuhui Pan, Ji Ma

“…Herein, single‐cell transcriptomics analysis is used to identify a unique malignant‐hepatoblast (HB)‐like cell subpopulation as the possible origin of poorly differentiated hepatoblastoma. …”
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Mechanical Load‐Induced Upregulation of Talin2 through Non‐Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis by Yizhen Huang, Heng Sun, Haojie Chen, Xiangpeng Wang, Junduo Zhao, Yang Jiao, Hongyi Zhou, Haoyu Cai, Jiafeng Dai, Xuan Huang, Weiyun Chen, Jianxiong Shen

“…Given that Talin2 is stabilized and deubiquitinated by OTUB1, especially under mechanical load, the Talin2/OTUB1 interaction may be a promising therapeutic target for FJOA.…”
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Elucidation of the role of stress-induced DNA polymerase II in the pathogenesis of uropathogenic Escherichia coli by Dr Damian Gawel, M.Sc. Anna Betkowska, MD Marcin Rozwadowski, Dr Marlena Godlewska, Prof Roel Schaaper

“…To evaluate the expression level of Pol II in the UPEC strains, SOS was induced by construction of UPEC/lexA(def) strains using the lambda red-mediated genome editing method. Whole transcriptome sequencing (RNA-seq) analysis was used to establish SOS-deregulated genes, including the expression level of polB, in the tested strains. …”
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Strike at the root: exploring the transferability of heat stress tolerance in tomatoes by reciprocal grafting by Robin T. Biermann, Robin T. Biermann, Linh T. Bach, Christian M. Steuer, Christian M. Steuer, Julia J. Reimer, Dietmar Schwarz, Frederik Börnke, Frederik Börnke

“…In addition to breeding, a promising approach to enhance HS tolerance is through grafting. …”
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PLIN2 promotes colorectal cancer progression through CD36-mediated epithelial-mesenchymal transition by Fan Yang, Ying Li, Xue Shang, Yun Zhu, Wenting Hou, Yi Liu, Qing Hua, Zhirong Sun

“…PLIN2 expression in monocytes/macrophages was verified by scRNA-seq datasets and spatial transcriptome datasets, and PLIN2 was found to promote macrophage transformation to M2 subtype. …”
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The FGF13‐Caveolin‐1 Axis: A Key Player in the Pathogenesis of Doxorubicin‐ and D‐Galactose‐Induced Premature Cardiac Aging by Enzhao Shen, Yuecheng Wu, Weijian Ye, Sihang Li, Junjie Zhu, Meifan Jiang, Zhicheng Hu, Gaoyong Cao, Xiaojing Yi, Fan Li, Zhouhao Tang, Xiaokun Li, Kwang Youl Lee, Litai Jin, Xu Wang, Weitao Cong

“…This study has demonstrated that FGF13 regulated the Cav1‐p53‐p21 axis to augment cardiomyocyte senescence and thereby exacerbated cardiac premature aging and suggests that FGF13 knockdown may be a promising approach to combat CVDs in response to aging and chemotoxicity.…”
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Novel Leech Antimicrobial Peptides, Hirunipins: Real‐Time 3D Monitoring of Antimicrobial and Antibiofilm Mechanisms Using Optical Diffraction Tomography by S. Dinesh Kumar, Jeongwon Park, Naveen Kumar Radhakrishnan, Yam Prasad Aryal, Geon‐Hwi Jeong, In‐Hyeok Pyo, Byambasuren Ganbaatar, Chul Won Lee, Sungtae Yang, Younhee Shin, Sathiyamoorthy Subramaniyam, Yu‐jin Lim, Sung‐Hak Kim, Seongsoo Lee, Song Yub Shin, Sung‐Jin Cho

“…Abstract Antimicrobial peptides (AMPs) are promising agents for treating antibiotic‐resistant bacterial infections. …”
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Anti-inflammatory therapy for tendinopathy using Il1rn mRNA encapsulated in SM102 lipid nanoparticles by Yuan Zhang, Yuan Zhang, Xu Li, Hao Li, Ruiyang Zhang, Ti Zhang, Talante Juma, Yongfei Zhou, Quanyi Guo, Hui Zhao, Yongping Cao

“…This SM102-Il1rn mRNA therapy enables spatiotemporally controlled anti-inflammatory therapy, providing a promising non-surgical solution for refractory tendinopathies. …”
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