Variation of nucleocapsid protein gene of infectious bronchitis virus and its expression in Escherichia coli by ZHOU Ji-yong, DING Hong-mei, CHENG Li-qin, Jimmy Kwang

“…This research was undertaken to investigate their variance where two specific primers were designed and synthesized according to the published sequence of N protein gene of IBV strain Beaudette. cDNAs of N gene were amplified by reverse transcription polymerase chain reaction (RT-PCR) from viral RNAs of IBV-ZJ971, N, X and H52 strains, cloned into vector pBluescript SK (+) and sequenced. …”
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A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression by Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi

“…Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) plays a crucial role in relative gene expression analysis, and accurate normalization relies on suitable reference genes (RGs). …”
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Cytoplasmic HMGB1 promotes the activation of JAK2-STAT3 signaling and PD-L1 expression in breast cancer by Ju-Young Han, Woo Joong Rhee, Jeon-Soo Shin

“…Statistical analyses were performed using Student’s t-tests, one-way analysis of variance, Pearson’s correlation, and Kaplan–Meier survival analysis, with significance set at p < 0.05. …”
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Alternative splicing induces sample-level variation in gene–gene correlations by Yihao Lu, Brandon L. Pierce, Pei Wang, Fan Yang, Lin S. Chen

“…Abstract Background The vast majority of genes in the genome are multi-exonic, and are alternatively spliced during transcription, resulting in multiple isoforms for each gene. …”
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Ensembling Classical Machine Learning and Deep Learning Approaches for Morbidity Identification From Clinical Notes by Vivek Kumar, Diego Reforgiato Recupero, Daniele Riboni, Rim Helaoui

“…Digital data exist in the form of images, video, speech, transcripts, electronic health records, clinical records, and free-text. …”
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Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging by Hu X, Du S, Chen M, Yang H, He J, Zhang L, Tan B, Wu T, Duan X

“…A miRNA network was created to look for target miRNAs connected with important genes, and transcriptional regulation analysis was used to identify related transcription factors. …”
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High-quality bonds: serine acetyltransferase 2 gene revealed by GWAS is associated with grain protein content in spring durum wheat by Aleksey Ermolaev, Ludmila Bespalova, Varvara Korobkova, Aleksey Yanovsky, Lubov Nazarova, Aleksandra Kroupina, Anastasia Chernook, Aleksandra Mudrova, Viktoria Voronezhskaya, Viktoria Voronezhskaya, Pavel Kroupin, Gennady Karlov, Mikhail Divashuk, Mikhail Divashuk

“…Expression profiling of TAsat2 homologous from bread wheat revealed peak transcription during grain filling stages, aligning with grain protein accumulation dynamics. …”
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Exploring the Causal Relationship Between Gut Microbiota and Pulmonary Artery Hypertension: Insights From Mendelian Randomization by Lihuang Su, Xinghong Wang, Ya Lin, Yiying Zhang, Dan Yao, Tongtong Pan, Xiaoying Huang

“…Methods and Results We examined the association between gut microbiota taxa and PAH using inverse variance weighted 2‐sample Mendelian randomization analysis, Mendelian randomization–Egger, weighted median, and weighted mode methods. …”
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Analytical Validation of the Cxbladder^® Triage Plus Assay for Risk Stratification of Hematuria Patients for Urothelial Carcinoma by Justin C. Harvey, David Fletcher, Charles W. Ellen, Megan Colonval, Jody A. Hazlett, Xin Zhou, Jordan M. Newell

“…Intra- and inter-assay variance was low, while extraction efficiency was high. …”
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Genome-wide association study for plant height and ear height in maize under well-watered and water-stressed conditions by Xin Wen, Hao-Yang Li, Ying-Lu Song, Peng-Yan Zhang, Zheng Zhang, Hua-Hu Bu, Chun-Lin Dong, Zhi-Qiang Ren, Jian-Zhong Chang

“…Among them, Zm00001d010175 and Zm00001d047994 encode an ethylene response transcription factor and inositol-tetrakisphosphate 1-kinase (ITPK1), respectively. …”
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Immunohistochemical Expression of Paired Box 2 (PAX2) in Normal Endometrium, Endometrial Hyperplasia, Endometrial Adenocarcinoma and its Association with Clinicopathological Parame... by HG Sowjanya, Vijaya V Mysorekar, BK Sujani

“…Paired box 2 (PAX2) is a member of the paired box gene family that is involved in transcriptional regulation during embryogenesis and has been found to mutate early during endometrial carcinogenesis. …”
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M2 macrophage-secreted KYNU promotes stemness remodeling and malignant behavior in endometrial cancer via the SOD2-mtROS-ERO1α-UPRER axis by Xin Pan, Wantong Wang, Yuting Wang, JiaHui Gu, Xiaoxin Ma

“…Additionally, ATF4 acted as a transcription factor in the KYNU promoter region, amplifying KYNU tumorigenesis in a positive feedback manner. …”
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Dissecting genomic regions and underlying candidate genes in groundnut MAGIC population for drought tolerance by Vinay Sharma, Supriya S. Mahadevaiah, Putta Latha, S. Anjan Gowda, Surendra S. Manohar, Kanchan Jadhav, Prasad Bajaj, Pushpesh Joshi, T. Anitha, Mangesh P. Jadhav, Shailendra Sharma, Pasupuleti Janila, Ramesh S. Bhat, Rajeev K. Varshney, Manish K. Pandey

“…Furthermore, important candidate genes associated with leaf senescence (NAC transcription factor), flowering (B3 domain-containing transcription factor, Ulp1 protease family, and Ankyrin repeat-containing protein), involved in chlorophyll biosynthesis (FAR1 DNA-binding domain protein), stomatal regulation (Rop guanine nucleotide exchange factor; Galacturonosyltransferases), and associated with yield traits (Fasciclin-like arabinogalactan protein 11 and Fasciclin-like arabinogalactan protein 21) were found in the vicinity of significant MTAs genomic regions. …”
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Optical quality changes of the eye during peak SARS-CoV-2 pandemic in young adults by He Tian, Qian Fan, Wenjing Gao, Yan Wang

“…Effects of varying SARS-CoV-2 infection levels were determined using reverse transcription polymerase chain reaction. Changes in corneal morphology, backscatter, and aberrations were measured. …”
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Efficacy of mindfulness-based intervention (‘mindfulness-based joyful sleep’) in young and middle-aged individuals with insomnia using a biomarker of inflammatory responses: a pros... by Chen Pan, Xiang Wang, Yunlong Deng, Peihuan Li, Yanhui Liao, Xin Ma, Guo-Ping Yang, Lisha Dai, Qiuping Tang

“…This study aims to compare the effectiveness of an MBI to CBT-I in young and middle-aged individuals with insomnia disorder and explore its effect on nuclear factor kappa B (NF-κB), a transcription factor that controls the expression of genes involved in inflammation.Methods and analysis This report describes a protocol for a randomised controlled trial. …”
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Epigenetic modifications are associated with mRNA and cytokine expression changes in chronic rhinosinusitis: a multiomics study from the United States by Devyani Lal, Tripti Brar, Chantal McCabe, Erik Jessen, Nitish Kumar, Pedro Lança Gomes, Michael J. Marino, Amar Miglani, Hirohito Kita

“…DNA methylation was the most significant data modality contributing to total variance on JDR. Cytokines critical in CRS (IL-5, IL-13, IL-10, IFNγ, IL-6) associated with hundreds of differentially methylated regions (DMRs) and mRNA. …”
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Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model by Hu J, Song Y, Zhang Y, Yang P, Chen S, Wu Z, Zhang J

“…Additionally, the expression of collagen type 1 (COL-1), ALP, and runt-related transcription factor-2 (RUNX-2) was evaluated through qRT-PCR and Western blot analysis. …”
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Impaired Compensatory Vasodilatory Effect Mediated by Wolfram Syndrome 1 and Corticotropin-Releasing Hormone Family Peptides in 17α-Ethynylestradiol-Induced Intrahepatic Cholestasi... by Tingting Xu, Daijuan Chen, Xixi Deng, Yongchi Zhan, Fan Zhou, Xiaodong Wang, Dandan Shi

“…Multivariate analysis of variance and least significant difference test were used to establish the group and individual comparisons. …”
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An mRNA expression atlas for the duck with public RNA-seq datasets by Qiuyu Tao, Anqi Huang, Jingjing Qi, Zhao Yang, Shihao Guo, Yinjuan Lu, Xinxin He, Xu Han, Shuaixue Jiang, Mengru Xu, Yuan Bai, Tao Zhang, Shenqiang Hu, Liang Li, Lili Bai, HeHe Liu

“…Additionally, we identified 27 low-variance, highly expressed housekeeping genes suitable for gene expression experiments. …”
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Analysis of abscisic acid-insensitive gene (ABI) sequence and phylogenetic relationship in genus Fagopyrum by WANG Yan, LIANG Chenggang, CHEN Qingqing, SHI Taoxiong, CHEN Qijiao, MENG Ziye, DENG Jiao, HUANG Juan, CHEN Qingfu

“…Among the families of ABA receptor genes, ABA- insensitive (ABI) is a major transcription factor that regulates ABA signal pathway. …”
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