Cytomegalovirus Worsens Necrotizing Enterocolitis Severity in Mice via Increased Toll-Like Receptor 4 SignalingSummary by Daniel Scheese, Peng Lu, Hannah Moore, Koichi Tsuboi, Cody Tragesser, Johannes Duess, Zachariah Raouf, Maame F. Sampah, Daphne Klerk, Mahmoud El Baassiri, Hee-seong Jang, Sierra Williams-McLeod, Asuka Ishiyama, Steve N. Steinway, Sanxia Wang, Menghan Wang, Thomas Prindle, Jr., William B. Fulton, Chhinder P. Sodhi, David J. Hackam

“…The adenosine receptor agonist 5'-N-ethylcarboxamido adenosine was tested for its ability to reduce CMV-induced effects on NEC severity. …”
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Cytokine screening identifies TNF to potentially enhance immunogenicity of pediatric sarcomas by Hendrik Gassmann, Hendrik Gassmann, Melanie Thiede, Jennifer Weiß, Emilie Biele, Luisa Flohé, Helena Lachermaier, Carolin Prexler, Valentina Evdokimova, Laszlo Radvanyi, Irfan Akhtar, Mina N. F. Morcos, Franziska Auer, Sebastian J. Schober, Julia Hauer, Julia Hauer, Uwe Thiel, Kristina von Heyking

“…Our previous study showed that mediators of monocyte maturation sensitized the EwS cell line A673 to lysis by HLA-A*02:01/CHM1319-specific allorestricted T cell receptor (TCR) transgenic CD8+ T cells (CHM1319 CD8+ T cells).MethodsIn this study, we tested a panel of monocyte maturation cytokines for their ability to upregulate immunogenic cell surface markers on OS, EwS and RMS cell lines, using flow cytometry. xCELLigence, SRB and ELISpot assays were used to assess whether TNF pretreatment increases CD8+ T cell cytotoxicity.ResultsWe observed that TNF and IL-1β upregulated MHC class I, ICAM-1 as well as CD83 and PD-L1 on the surface of pediatric sarcoma cell lines, while IL-4, GM-CSF, IL-6 and PGE2 failed to induce respective effects. …”
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C-reactive protein-to-prealbumin ratio as prognostic marker in hemodialysis patients with Omicron infection by Quanchao Zhang, Caibao Lu, Zhifen Wu, Shaofa Wu, Lili Jiang, Han Wang, Jin He, Bingshuang Tang, Bingfeng Yang, Shengli Liao, Liao Wang, Hongwei Chen, Moqi Li, Wenchang He, Yiqin Wang, Jie Li, Jinghong Zhao, Ling Nie

“…In addition, we also found that the CP ratio has better discriminatory ability, than the C-reactive protein to albumin ratio (areas under the curve; 0.84 vs. 0.80; Z-test; p = 0.001).Conclusions This study underscores that CP ratio substantially influence the mortality rates in hemodialysis patients following Omicron infection. …”
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Antibacterial and Inhibitory Activity of Nora and Mepa Efflux Pumps of Estragole Complexed to β-Cyclodextrin (ES/β-CD) In Vitro Against <i>Staphylococcus aureus</i> Bacteria, Molec... by Roger Henrique Sousa da Costa, Renata Torres Pessoa, Eduardo dos Santos Silva, Isaac Moura Araujo, Sheila Alves Gonçalves, Janaína Esmeraldo Rocha, Francisco Nascimento Pereira Junior, Naiara Cipriano Oliveira, Victor Moreira de Oliveira, Matheus Nunes da Rocha, Emmanuel Silva Marinho, Natália Kelly Gomes de Carvalho, José Galberto Martins da Costa, Hélcio Silva dos Santos, Irwin Rose Alencar de Menezes

“…Methodology: Several compounds and antibiotics, such as ciprofloxacin and norfloxacin, were used to evaluate the antimicrobial activity and the ability of the ES/β-CD complex to reverse resistance. …”
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RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells by Maryann Batiste, Bethany Joy, Cara K. Yee, Luke Cho, Ashley Christensen, Ihab Abed, Kailey Nguyen, Anusri Yanumula, Hannah Chang, Evan D. Cho, Wenjia Wang, Emily Chou, Esther H. Chang, Yennie L. Shyu, Alyssa Abram, Jessa Alcaide, James Zhou, Brittany Gillespie, Michelle Senderovich, Gianne Almeida Cusick, Ai-Vy Le, Frank Hoang, Yihui Shi, Eslam Mohamed, John K. Cusick

“…RELT localization was detected through immunofluorescence and western blotting, and co-immunoprecipitation was used to test whether a mutated RELT interacts with the OXSR1 kinase. …”
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Identification and quantification of transposable element transcripts using Long-Read RNA-seq in Drosophila germline tissues by Rebollo, Rita, Gerenton, Pierre, Cumunel, Eric, Mary, Arnaud, Sabot, François, Burlet, Nelly, Gillet, Benjamin, Hughes, Sandrine, S. Oliveira, Daniel, Goubert, Clément, Fablet, Marie, Vieira, Cristina, Lacroix, Vincent

“…Most expressed TE insertions correspond to copies that have lost their ability to transpose, and within a family, only a few copies are indeed expressed. …”
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Effects of liver-specific LCMT1 gene knockout on glycolipid metabolism, learning and memory in mice by CHEN Sihong, TANG Tingting, CAI Haiqing, WANG Xinhang, WANG Minghong, PENG Yang, ZHANG Minhua, WEI Meifang, ZHU Congrui, ZHANG Jing, LI Xiaoying, LU Cailing, TANG Shen, LI Xiyi

“…At 16 weeks of age, the motor ability and spatial learning and memory abilities of the two groups of mice were assessed using the fatigue rotating rod test and the Morris water maze test, respectively. …”
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Effective intravenous delivery of adenovirus armed with TNFα and IL-2 improves anti-PD-1 checkpoint blockade in non-small cell lung cancer by Tatiana V. Kudling, James H.A. Clubb, Santeri Pakola, Dafne C.A. Quixabeira, Iris A.K. Lähdeniemi, Camilla Heiniö, Victor Arias, Riikka Havunen, Victor Cervera-Carrascon, Joao M. Santos, Eva Sutinen, Jari Räsänen, Kristian Borenius, Mikko I. Mäyränpää, Eero Aaltonen, Suvi Sorsa, Otto Hemminki, Anna Kanerva, Emmy W. Verschuren, Ilkka Ilonen, Akseli Hemminki

“…Therefore, there is a major unmet clinical need for checkpoint refractory/resistant NSCLC. Here, we tested the combination of aPD-1 and adenovirus armed with TNFα and IL-2 (Ad5-CMV-mTNFα/mIL-2) in an immunocompetent murine NSCLC model. …”
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A predictive nomogram for assessing the likelihood of retrieving 12 lymph nodes after rectal cancer surgery: a single-center study by Jian Ma, Runyang Hao, Shuai Jiao, Qingmin Chen, Baohong Yang, Xu Guan, Jiale Li, Xinxuan Zhao, Yu Huo, Qingxia Xu, Haiyi Liu, Wen Su, Xishan Wang

“…Continuous variables were converted into categorical variables. Chi-square tests were used to identify key factors influencing the retrieval of 12 LNs. …”
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Development of a cost‐effective high‐throughput mid‐density 5K genotyping assay for germplasm characterization and breeding in groundnut by Manish K. Pandey, Vinay Sharma, Aamir W. Khan, Pushpesh Joshi, Sunil S. Gangurde, Prasad Bajaj, Pasupuleti Janila, Annapurna Chitikineni, Ramesh Bhat, Babu N. Motagi, Chandramohan Sangh, Thankappan Radhakrishnan, Sandip K. Bera, Gregor Gorjanc, Krishna Reddy Gujjula, Nathan Hall, Claudio D. Carrasco, Kandalam Arjun, Srinivas Chandram, Rajeev K. Varshney

“…The mid‐density assay demonstrated a proficient ability to distinguish between the genotypes, offering a high level of genome‐wide nucleotide diversity. …”
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Prediction model of ocular metastasis from primary liver cancer: Machine learning‐based development and interpretation study by Jin‐Qi Sun, Shi‐Nan Wu, Zheng‐Lin Mou, Jia‐Yi Wen, Hong Wei, Jie Zou, Qing‐Jian Li, Zhao‐Lin Liu, San Hua Xu, Min Kang, Qian Ling, Hui Huang, Xu Chen, Yi‐Xin Wang, Xu‐Lin Liao, Gang Tan, Yi Shao

“…The extreme gradient boost (XGB) ML model achieved the optimal differential diagnosis ability, with an area under the curve (AUC) = 0.993, accuracy = 0.992, sensitivity = 0.998, and specificity = 0.984. …”
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Developing a model for decision-making around antibiotic prescribing for patients with COVID-19 pneumonia in acute NHS hospitals during the first wave of the COVID-19 pandemic: qua... by Neil Powell, Emma Thomas-Jones, Paul Dark, Tamas Szakmany, Susan Hopkins, Jonathan Sandoe, Margaret Ogden, Robert M West, Enitan Carrol, Lucy Brookes-Howell, Philip Pallmann, Bethany Shinkins, Dominick Shaw, Philip Howard, Stacy Todd, Thomas P Hellyer, David G Partridge, Graham Prestwich, Sarah M Gerver, Mahableshwar Albur, Martin Llewelyn, Joanne Euden, Iain J McCullagh, Josie Henley, Ryan Hamilton, Wakunyambo Maboshe, Stuart E Bond, Helena Parsons

“…However, as clinicians gained more experience with COVID-19, they reported increasing confidence in their ability to distinguish between symptoms and signs caused by SARS-CoV-2 viral infection alone, and secondary bacterial infections. …”
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Biochar effects on durum wheat (Triticum durum) under ambient and elevated atmospheric CO2 by Lorenzo Brilli, Franz-Werner Badeck, Lorenzo Genesio, Silvia Baronti, Fulvia Rizza, Clara Fares, Luigi Cattivelli, Franco Miglietta, Anita Maienza, Alessandro Zaldei, Federica Camin, Rosella Motzo, Piero Toscano

“…The use of biochar as amendment, thanks to its well-known ability to improve soil fertility and crop production and quality, could be a suitable solution to cope with the negative effect of e[CO2] on qualitative traits. …”
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Integrating multiple terrain features for artefact detection in the newly released TanDEM-X 30 m DEM and DCM over the Loess Plateau by Xingang Zhang, Shanchuan Guo, Zilong Xia, Haowei Mu, Bing Wang, Bin Cui, Hong Fang, Peijun Du

“…Specifically, twelve terrain features (including slope, roughness, second-order derivatives, etc.) were selected for their ability to discriminate artefacts, and a CatBoost model is implemented for artefact detection. …”
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Prognostic impact of dynamic changes of type I melanoma antigen gene proteins CT7 (MAGE-C1/CT7) transcripts in multiple myeloma by Xuelin Dou, Fengrong Wang, Huan Chen, Yao Chen, Lei Wen, Yang Liu, Guorui Ruan, Xiaosu Zhao, Xiaojun Huang, Robert Peter Gale, Jin Lu, Jin Lu

“…In univariate Cox regression analysis, peri-ASCT MAGE-C1/CT7 status showed better discriminatory ability in PFS and survival than peri-ASCT multi-parameter flow-cytometry status assessed by flow cytometry. …”
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Odor induced functional connectivity alteration of POC-anterior frontal cortex-medial temporal cortex in patients with mild cognitive impairment by Weiping Li, Jianan Zhou, Shuying Li, Min Wu, Yajing Zhu, Qian Chen, Futao Chen, Xuefeng Ma, Xin Zhang, Zhengge Wang, Jiaming Lu, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang, Bing Zhang

“…All subjects underwent cognitive assessments, olfactory behavior tests, and odor-based functional magnetic resonance imaging (fMRI). …”
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Monitoring poultry social dynamics using colored tags: Avian visual perception, behavioral effects, and artificial intelligence precision by Florencia B. Rossi, Nicola Rossi, Gabriel Orso, Lucas Barberis, Raul H. Marin, Jackelyn M. Kembro

“…In separate experiments, we aim to: (i) evaluate avian visual perception of the different colored tags; (ii) assess the potential impact of tag colors on social behavior; and (iii) test the ability of the YOLO model to accurately distinguish between different colored tags on Japanese quail in social group settings. …”
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Exploring the Antimycobacterial Potential of Podocarpusflavone A from <i>Kielmeyera membranacea</i>: In Vitro and In Vivo Insights by Marlon Heggdorne de Araujo, Salomé Muñoz Sánchez, Thatiana Lopes Biá Ventura Simão, Natalia Nowik, Stella Schuenck Antunes, Shaft Corrêa Pinto, Davide Sorze, Francesca Boldrin, Riccardo Manganelli, Nelilma Correia Romeiro, Elena B. Lasunskaia, Fons J. Verbeek, Herman P. Spaink, Michelle Frazão Muzitano

“…In addition, the EE, DCM, and PCFA have shown the ability to inhibit <i>M. marinum’s</i> growth during in vivo zebrafish larvae yolk infection. …”
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C3d-targeted complement inhibitors to correct complement dysregulation in aHUS patients by Valeria Guaschino, Donata Santarsiero, Sara Gastoldi, Joshua M. Thurman, V. Michael Holers, Shelia M. Violette, Fei Liu, Kelly C. Fahnoe, Chiara Guarinoni, Ariela Benigni, Giuseppe Remuzzi, Marina Noris, Sistiana Aiello

“…We also assessed their ability to prevent loss of anti-thrombogenic properties in HMEC-1 pre-exposed to aHUS serum and then perfused with control blood. …”
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Enhancing the nutritional profile of purple sweet potato flour through fermentation with Lactiplantibacillus plantarum InaCC B157: A functional food perspective by Tiurma Pasaribu, Yati Sudaryati Soeka, Novik Nurhidayat, Suciatmih Suciatmih, Titin Yulinery, Evi Triana, Tri Ratna Sulistiyani, Ninik Setyowati, Desty Dwi Sulistyowati, Dwi Ningsih Susilowati

“…This study aimed to evaluate the ability of Lactiplantibacillus plantarum InaCC B157 to enhance the biochemical and functional composition of purple sweet potato flour. …”
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