Extraordinary bloom of toxin-producing phytoplankton enhanced by strong retention on the offshore Patagonian shelf by V. A. Guinder, U. Tillmann, M. Rivarossa, C. Ferronato, F. J. Ramírez, B. Krock, H. Gu, M. Saraceno, M. Saraceno, M. Saraceno

“…The toxin azaspiracid-2 (AZA-2) was detected in both stages of the bloom, with values up to 2122 pg L<span class="inline-formula">⁻¹</span>. …”
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High HBsAg clearance rate and viral dynamics in HBeAg-positive, ALT-normal children and adolescents with chronic HBV infection: results from the prospective sprout project by Xin Wang, Changxiang Lai, Ruiling Li, Jia Lei, Yuyin Xie, Zhiyu Li, Qiyuan Tang, Qing He, Hongfei Zhang, Zheng Zhang, Fang Wang

“…Notably, 61.54% developed detectable HBsAb prior to HBsAg loss. Children aged 3–7 years had significantly higher clearance rates than those aged 8–18 years. …”
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Tregs levels and phenotype modifications during Amyotrophic Lateral Sclerosis course by Elisabetta Zucchi, Elisabetta Zucchi, Federico Banchelli, Cecilia Simonini, Sara De Biasi, Ilaria Martinelli, Giulia Gianferrari, Giulia Gianferrari, Domenico Lo Tartaro, Andrea Cossarizza, Andrea Cossarizza, Roberto D’Amico, Roberto D’Amico, Jessica Mandrioli, Jessica Mandrioli

“…Additionally, we explored whether measures of ALS progression, such as the decline over time in the revised ALS Functional Rating Scale (ALSFRS-r) or forced vital capacity (FVC) correlated Treg levels and whether Treg phenotype varied during the course of ALS.MethodsTotal Tregs (detected by CD3, CD4, FoxP3, CD25, and CD127) were quantified at five time points over 54 weeks in 21 patients in the placebo arm of the RAP-ALS trial; next they were characterized for the expression of surface markers including CD38, CD39, CXCR3, and PD1. …”
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UVC-PURGE: A Novel Cost-Effective Disinfection Robot for Combating COVID-19 Pandemic by Akib Zaman, Mohammad Shahjahan Majib, Shoeb Ahmed Tanjim, Shah Md. Ahasan Siddique, Shafayetul Islam, Md Shadman Aadeeb, Nafiz Imtiaz Khan, Riasat Haque, Md Rashid Ul Islam, M. Rayhan Ferdous Faisal, Siddharth Malik, Muhammad Nazrul Islam

“…Pre-treatment swab testing revealed the presence of both Gram-positive and Gram-Negative bacteria at 17 out of 20 test surfaces in the conducted tests. After the UVC irradiation of the robot, the microbial load was detected in only 2 (1D and 1H) out of 17 test surfaces with significant reductions (95.33&#x0025; in 1D and 90.9&#x0025; in 1H) of microbial load. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…To address this limitation, we investigated an alternative staining method employing calcein acetoxymethyl (CAM) to detect viable cells based on esterase activity, and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH) to assess the remaining biofilm population.MethodsBiofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecium were matured and exposed to varying concentrations of antibiotics or sterile medium. …”
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QTL mapping of seedling biomass and root traits under different nitrogen conditions in bread wheat (Triticum aestivum L.) by Meng-jiao YANG, Cai-rong WANG, Muhammad Adeel HASSAN, Yu-ying WU, Xian-chun XIA, Shu-bing SHI, Yong-gui XIAO, Zhong-hu HE

“…SNP markers tightly linked to two important QTLs clusters C10 and C11 on chromosomes 6BL and 7BL were converted to kompetitive allele-specific PCR (KASP) assays that underpin important traits in root development, including root dry weight, root surface area and shoot dry weight. These QTLs, clusters and KASP assays can greatly improve the efficiency of selection for root traits in wheat breeding programmes.…”
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Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome by Jiao Wang, Guang-Hong Chu, Zi-Huan Wang, Xiao-Yu Cai, Si-Yuan Shi, Qi-Ping Qing, Qi Zhang

“…Serological indicators, including antinuclear antibodies (ANA) and anti-Ro60, anti-Ro52, and anti-La autoantibodies, were detected. Statistical analyses evaluated correlations between IRFs expression and clinical parameters. …”
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The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis by Julia A. Alvarez, Elisabet Gas-Pascual, Sahil Malhi, Juan C. Sánchez-Arcila, Ferdinand Ngale Njume, Hanke van der Wel, Yanlin Zhao, Laura García-López, Gabriella Ceron, Jasmine Posada, Scott P. Souza, George S. Yap, Christopher M. West, Kirk D. C. Jensen

“…ABSTRACT Glycosylphosphatidylinositols (GPIs) are highly conserved anchors for eukaryotic cell surface proteins. The apicomplexan parasite, Toxoplasma gondii, is a widespread intracellular parasite of warm-blooded animals whose plasma membrane is covered with GPI-anchored proteins, and free GPIs called GIPLs. …”
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Novel and potent MICA/B antibody is therapeutically effective in KRAS LKB1 mutant lung cancer models by Shohei Koyama, Yujiro Naito, John D Minna, David E Gerber, Ryan R Kowash, Manoj Sabnani, Laura T Gray, Qing Deng, Nusrat U A Saleh, Luc Girard, Kentaro Masahiro, Zhiqian Lucy Liu, Hemanta Baruah, Esra A Akbay

“…Soluble MICA/B is also detected in patient blood samples. AHA-1031 binds to the α3 domain of MICA/B, preventing shedding and targeting tumor cells to ADCC. …”
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An Acinetobacter baumannii nasal carriage isolate recovered from an asymptomatic patient in Vietnam is extensively antibiotic resistant and produces a rare K71 type capsule by Anna M. Shpirt, Christopher J. Harmer, Alexander S. Shashkov, Mikhail M. Shneider, Alexander O. Chizhov, Andrey S. Dmitrenok, Anastasiya V. Popova, Anastasiya A. Kasimova, Andrei V. Perepelov, Yuriy A. Knirel, Ruth M. Hall, Johanna J. Kenyon

“…We report the resistance profile and correlate this with detected resistance determinants. We also solve the structure of the CPS and reveal its relationship with CPS produced by other A. baumannii isolates.…”
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Migration history of Avicennia marina populations: a legacy of mangrove expansion on the Sunda Shelf by Ludwig Triest, Thi Thuy Hang Phan, Quang Doc Luong, Anne Bousquet-Mélou, Bich Thi Ngoc Do, Tim Sierens, Farid Dahdouh-Guebas, Farid Dahdouh-Guebas, Farid Dahdouh-Guebas, Nico Koedam, Nico Koedam, Nico Koedam, Tom Van der Stocken

“…Avicennia species exhibit a pronounced genetic structure following a stepping-stone migration model, with connectivity patterns linked to the strength and direction of ocean-surface currents. The present-day spatial genetic structure of Avicennia marina populations is an imprint of connectivity. …”
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The Value of Hydrogen Peroxide in Neurosurgery and Its Pathophysiological Effects in Human and Animal Brain Tissues by Violetta C. Spoeler, Markus Kipp, Daniel Dubinski, Joshua D. Bernstock, Artem Rafaelian, Svorad Trnovec, Cajetan I. Lang, Thomas M. Freiman, Sami Ridwan, Friedrich Prall, Florian Gessler, Sae-Yeon Won

“…In human brain tissues, vacuolization was detected in sections exposed to H₂O₂ 1.5% and 3%, with a mean depth of damage of 543.8 ± 304.5 μm and 859.0 ± 379 μm (<i>p</i> = 0.003). …”
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Water vapour isotopes over West Africa as observed from space: which processes control tropospheric H₂O&thinsp;∕&thinsp;HDO pair distributions? by C. J. Diekmann, C. J. Diekmann, M. Schneider, P. Knippertz, T. Trent, T. Trent, H. Boesch, H. Boesch, A. N. Roehling, J. Worden, B. Ertl, B. Ertl, F. Khosrawi, F. Khosrawi, F. Hase

“…</p> <p>This study is the first to apply comprehensive isotopic datasets from IASI, TROPOMI and AIRS to the WAM, demonstrating the utility of satellite-based {H<span class="inline-formula">₂</span>O, <span class="inline-formula"><i>δ</i>D</span>} pairs in detecting impacts of microphysical and dynamical processes on water vapour isotopic composition.…”
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Biosynthesis and activity of Zn-MnO nanocomposite in vitro with molecular docking studies against multidrug resistance bacteria and inflammatory activators by Samy Selim, Tarek M. Abdelghany, Mohammed S. Almuhayawi, Mohammed K. Nagshabandi, Muyassar K. Tarabulsi, Mohammed Yagoub Mohammed Elamir, Asmaa A. Alharbi, Soad K. Al Jaouni

“…Numerous functional groups were detected on the surface of Zn-MnO nanocomposite via Fourier-transform infrared spectroscopy. …”
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Mechanisms and network pharmacological analysis of Yangyin Fuzheng Jiedu prescription in the treatment of hepatocellular carcinoma by Yuqing Xie, Fengna Yan, Xinhui Wang, Lihua Yu, Huiwen Yan, Qing Pu, Weihong Li, Zhiyun Yang

“…The production of serum cytokines was detected using the Milliplex® MAP mouse high sensitivity T cell panel kit. …”
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Correlation between cardiac index(CI) and cardio ankle vascular index(CAVI) at different degrees of head up tilt table by Akeel Zwain, Ahlam Abood

“…A new index CI/CAVI may be proposed; it can be employed to early detect subclinical atherosclerosis impact on cardiac muscle pump function. …”
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Effects of CVOCs on Low-Temperature Adsorption of Elemental Mercury by Adsorbents by Qinyuan HONG, Xiangling CAI, Guanqun GAO, Haomiao XU, Zan QU, Naiqiang YAN

“…Brunauer-Emmett-Teller (BET) analysis indicated no direct relationship between the specific surface area or pore type of the adsorbents and their Hg0 adsorption performance. …”
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CD80 Antibody and MTX Co-Engineered Extracellular Vesicles Targets CD80+ Macrophages to Suppress Inflammation and Alleviate Chronic Inflammatory Diseases by Yang J, Zhang H, Wang W, Yin Q, He X, Tao D, Wang H, Liu W, Wang Y, Dong Z, Chen X, Li B

“…However, there are no definitive therapies to reshape the tissue homeostasis between innate immune and adaptive immune cells.Methods: (1) anti-CD80-MTX-EVs was obtained by gradient centrifugation, which were characterized by TEM and DLS, and the associated membrane proteins were identified by Western Blot. (2) The mouse bone marrow-derived macrophages were co-cultured separately with EVs, anti-CD80-EVs, and anti-CD80-MTX-EVs in vitro, and the expression of CD80 on the macrophages surface as well as the proportion of Treg cell generation were detected. (3) EVs, anti-CD80-EVs and anti-CD80-MTX-EVs were injected into mice models of arthritis and periodontitis for treatment, the therapeutic effect was evaluated by the expressions of related cytokines, staining of HE, the proportion of CD80+ macrophages and the phenotypic differentiation of T cells in the tissues.Results: We successfully constructed engineered EVs (anti-CD80-MTX-EVs) targeting inflammatory macrophages for intracellular MTX delivering, which inducing the anti-inflammatory transformation while upregulating the expression of TGF-β of macrophages. …”
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DEPENDENCE OF CHANGES IN THE HYGIENIC CONDITION OF THE ORAL CAVITY AND PERIODONTIUM IN CHILDREN WITH MALIGNANT TUMORS ON THE DURATION OF POLYCHEMYTHERAPY AND QUANTITIES OF CYTOSTAT... by Yu.V. Popelo

“…Localized erosions covered with fibrinous plaque were detected in 4 of these patients (20,0%) on the background of erythema. …”
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Material Characterization and Technological Properties of Biocompatible Ti-12Al-42Nb Spherical Powder Alloy for Additive Manufacturing of Personal Medical Implants by Alexander Anokhin, Andrey Kirsankin, Elena Kukueva, Alexander Luk’yanov, Maria Chuvikina, Elena Ermakova, Svetlana Strelnikova, Stepan Kupreenko

“…Simultaneous thermal analysis (STA) was performed to indicate weight growth and losses and thermal effects in argon, nitrogen, and air as well as the oxidation of Al₂O₃, TiO₂, and Nb₂O₅ on the surface layer of Ti-12Al-42Nb powder alloy particles. …”
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