Computer-Assisted, Quantitative Cytokine Enzyme-Linked Immunospot Analysis of Human Immune Effector Cell Function by Yan Cui, Lung-Ji Chang

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Optimizing one-leg standing test duration for screening functional decline in community-dwelling older adults by Akira Iwata, Izumi Arihara, Keita Sasada, Atsuki Kanayama, Kenichiro Tsubokura, Gaito Kitada, Ryoga Ueba, Shuji Okuno, Toshimitsu Ohmine, Saki Yamamoto

“…They performed the one-leg standing test for a maximum of 60 s and were categorized into those who could or could not maintain the position at each time point. Physical function was assessed through mobility (gait speed and five times sit-to-stand test), balance (Timed Up and Go (TUG) test and two-step test), and knee extension strength. …”
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Phenotype and function of human monocytes remain mainly unaffected by very small superparamagnetic iron oxide particles by Sari Hussein, Sari Hussein, Anja Andrea Kühl, Laura Golusda, Laura Golusda, Christina Plattner, Nadine Heinze, Nadine Heinze, Gregor Sturm, Christian Freise, Heike Traub, Mathias Schannor, Zlatko Trajanoski, Matthias Taupitz, Britta Siegmund, Daniela Paclik, Daniela Paclik

“…This study examines nanoparticle effects on human blood cells focusing on monocytes in vitro as a first step toward clinical application. Whole blood and monocytes from healthy donors and patients with inflammatory bowel disease were treated with VSOP in vitro and analyzed for changes in their transcriptome, phenotype and function. …”
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Strain promoted azide alkyne cycloaddition, an efficient surface functionalization strategy for microRNA magnetic separation by Djamila Kechkeche, Sirine El Mousli, Claire Poujouly, Emilie Secret, Vincent Dupuis, Isabelle Le Potier, Marie-Emmanuelle Goriot, Julien Siracusa, Sébastien Banzet, Jean Gamby, Jean-Michel Siaugue

“…In an emergency context, the detection of microRNA (miRNA), which are potential biomarkers of cardiovascular and muscular pathologies is hampered by the time required for the various steps involved: treatment of the patient sample, reverse transcription (RT), and finally polymerase chain reaction (PCR). …”
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The Algorithm for Recognizing Superposition of Wave Aberrations from Focal Pattern Based on Partial Sums by Sergey G. Volotovsky, Pavel A. Khorin, Aleksey P. Dzyuba, Svetlana N. Khonina

“…It is numerically shown that when recognizing superpositions of Zernike functions from the intensity pattern in the focal plane, the use of step-by-step optimization in combination with the Levenberg–Marquardt algorithm yields good results only with an initial approximation close to the solution. …”
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Investigation and functional characterization of rare genetic variants in the adipose triglyceride lipase in a large healthy working population. by Stefan Coassin, Martina Schweiger, Anita Kloss-Brandstätter, Claudia Lamina, Margot Haun, Gertraud Erhart, Bernhard Paulweber, Yusof Rahman, Simon Olpin, Heimo Wolinski, Irina Cornaciu, Rudolf Zechner, Robert Zimmermann, Florian Kronenberg

“…We therefore screened the full genomic region of ATGL for mutations in 1,473 randomly selected individuals from the SAPHIR (Salzburg Atherosclerosis Prevention program in subjects at High Individual Risk) Study using a combined Ecotilling and sequencing approach and functionally investigated all detected protein variants by in-vitro studies. …”
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The dynamics of microbial community structure and metabolic function in different parts of cigar tobacco leaves during air-curing by Wenlong Li, Wenlong Li, Jun Yu, Jun Yu, Hao Li, Hao Li, Chunlei Yang, Chunlei Yang, Zheng Peng, Zheng Peng, Juan Zhang, Juan Zhang

“…Air-curing is the initial step in the processing of cigar tobacco leaves. However, the dynamics of microbial community and metabolic functions in different parts of tobacco leaves during this process remain largely unclear. …”
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Fecal carriage of vanB antibiotic resistance gene affects adipose tissue function under vancomycin use by Lars M. M. Vliex, Giang N. Le, Marina Fassarella, Dorien Reijnders, Gijs H. Goossens, Erwin G. Zoetendal, John Penders, Ellen E. Blaak

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Dioctylsulfosuccinate Functionalized NiAl-Layered Double Hydroxide for Sensitive Fenuron Electroanalysis Using a Carbon Paste Electrode by Aude Peggy Kameni Wendji, Herve Leclerc Tcheumi, Ignas Kenfack Tonle, Emmanuel Ngameni

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Preferential Stripping Analysis of Post-Transition Metals (In and Ga) at Bi/Hg Films Electroplated on Graphene-Functionalized Graphite Rods by Nastaran Ghaffari, Nazeem Jahed, Zareenah Abader, Priscilla G. L. Baker, Keagan Pokpas

“…The improved adsorption of Ga³⁺ and In³⁺ ions at the Bi/Hg films on the graphene-functionalized electrodes during the preconcentration step significantly enhanced sensitivity, achieving detection limits of 2.53 nmol L⁻¹ for Ga³⁺ and 7.27 nmol L⁻¹ for In³⁺. …”
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Protocol for isolating cervico-vaginal fluid cells from Macaca mulatta to study immunological and functional changes during pregnancy by Daniel Short, Monica Cappelletti, Anne Vu, Mark R. Johnson, Suhas G. Kallapur, Pietro Presicce

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Impact of Venous CONgestion on Organ Function and Outcomes in Sepsis (ICON-Sepsis): a prospective observational cohort study protocol by Hao Ying, Robert R. Ehrman, Robert L. Sherwin, Christian A. Reynolds, Steven J. Korzeniewski, Robert D. Welch, Jeffrey A. Kline, Phillip D. Levy

“…Patient-level heterogeneity in prior sepsis trials is recognised as a substantial contributor to all these problems, yet no prior investigation has attempted to identify volume-informed septic phenotypes, a necessary first step towards precision care.Methods and analysis Predicated on prior studies demonstrating detectability of organ-level congestion, we hypothesise that central venous hypertension (1) is deleterious to the function of the lungs, liver, kidneys and vascular endothelium; (2) is worsened by cardiac dysfunction and IVF administration; and (3) contributes to adverse organ-specific and overall outcomes. …”
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High-intensity intermittent exercise regulates oxidative stress and improves endothelial progenitor cell function in patients with essential hypertension by Jixin ZHI, Tiantian WANG, Shuang REN, Chenyu WANG

“…Before and after intervention, brachial artery flow-mediated vasodilation (FMD) was used to evaluate vascular endothelial function; venous blood was sampled to perfrom circulating endothelial progenitor cell counts; endothelial progenitor cells were cultured in vitro, and the modified Boyden chamber assay and Matrigel lumen formation assay were used to detect their migration and tube formation ability, superoxide fluorescent anion probe method to detect reactive oxygen species levels, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining method to detect cell apoptosis, Western blotting to determine protein expression of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2, NADPH oxidase 4, and superoxide dismutase. …”
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A comparative analysis of single-step and multi-step methods for producing magnetic activated carbon from palm kernel shells: Adsorption of methyl orange dye by Waluyo Joko, Rahmawati Farida Dwi, Izzulhaq Muhammad Ghozy, Purba Ibnu Tryansar, Kaavessina Mujtahid, Wibowo Wusana Agung, Pranolo Sunu Herwi, Buwono Haris Puspito, Septian Ardie, Adnan Muflih Arisa

“…Fourier transform infra-red characterization showed that the functional groups detected on the activated carbon and MAC were O–H, C═O, C═C, C≡N, and C–O. …”
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Adaptation of an Eddy Current Model for Characterizing Subsurface Defects in CFRP Plates Using FEM Analysis Based on Energy Functional by Mario Versaci, Filippo Laganà, Francesco Carlo Morabito, Annunziata Palumbo, Giovanni Angiulli

“…The adapted EC model admits a unique and stable solution by verifying that all analytical steps are satisfied. To reconstruct 2D maps of the magnetic flux density amplitude, a FEM formulation is adopted, based on the energy functional because it ensures a stable and consistent numerical formulation given its coercivity. …”
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Using an ensemble approach to predict habitat of Dusky Grouse ( Dendragapus obscurus ) in Montana, USA by Elizabeth A Leipold, Claire N Gower, Lance McNew

“…Our goal was to explore relationships between Dusky Grouse use and habitat characteristics, and then generate a state-wide map predicting Dusky Grouse habitat in Montana using two methods: resource selection functions and random forest classifiers. The Integrated Monitoring in Bird Conservation Regions program provided a multi-year dataset of Dusky Grouse observations, which we reduced to detected (n=132) and pseudo-absent (n=5960) locations, using geospatial datasets to obtain topographic and vegetation characteristics for each location. …”
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Convolutional networks can model the functional modulation of the MEG responses associated with feed-forward processes during visual word recognition by Marijn van Vliet, Oona Rinkinen, Takao Shimizu, Anni-Mari Niskanen, Barry Devereux, Riitta Salmelin

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Effects of branched-chain amino acid- and vitamin D-containing high-protein food supplementation plus exercise on elderly people with decreased physical functions by Yoshihiro Nakamura, Takuji Yokoe, Koki Ouchi, Taro Funamoto, Hiroyuki Kimura, Koichiro Hamada, Hideki Arakawa, Etsuo Chosa, Naosuke Kamei

“…Conclusions This study showed that BCAA and vitamin D supplementation with exercise in elderly subjects with declining physical functions resulted in maintenance of soft lean mass, improvement in physical functions (stand-up test score and two-step test), and an increase in 25OH vitamin D. …”
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A multistage Plasmodium CRL4WIG1 ubiquitin ligase is critical for the formation of functional microtubule organization centers in microgametocytes by Ravish Rashpa, Cameron Smith, Katerina Artavanis-Tsakonas, Mathieu Brochet

“…However, little is known about the repertoire and function of these enzymes in Plasmodium. Here, we show that Plasmodium expresses a conserved cullin RING E3 ligase (CRL) complex that is functionally related to CRL4 in other eukaryotes. …”
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What do we know about nursing practice in relation to functional ability limitations, frailty and models of care among older people in home- and facility-based care: a scoping revi... by Ida Røed Flyum, Edith Roth Gjevjon, Anna Josse Eklund, Gunilla Borglin

“…Abstract Background Nursing practice in long-term care, must support the delivery of safe and evidence-based care, especially for older people with functional ability limitations and frailty, with the competency, knowledge and structured working modes such practice requires. …”
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