Frequent sgRNA-barcode recombination in single-cell perturbation assays. by Shiqi Xie, Anne Cooley, Daniel Armendariz, Pei Zhou, Gary C Hon

“…Simultaneously detecting CRISPR-based perturbations and induced transcriptional changes in the same cell is a powerful approach to unraveling genome function. …”
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Machine learning-enabled multiscale modeling platform for damage sensing digital twin in piezoelectric composite structures by Somnath Ghosh, Saikat Dan, Preetam Tarafder

“…The DT framework consists of a two-step computational process integrating multiscale-multiphysics modeling with machine learning (ML) tools to detect damage progression in the piezoelectric composite structure using electrical signal measurements at a few surface points. …”
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Bioactives and Other Nutritional Components in the Jaggery Production Process by Nisha Pujari, Nirali Dedhia, Sanjay Mahajani, Narendra Shah, Amit Arora

“…Some unreported phenolics and flavonoids, such as isoferulic acid, prunitrin and maritimetin, which are known to have anti-inflammatory properties, were detected in selected NCS samples. In order to retain maximum mineral content in jaggery and to ensure the removal of appropriate amounts of scum, technological upgrades must be studied and the clarification step must be optimized and standardized.…”
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Counseling an alcohol abuse patient by Zh. V. Maksimova, D. M. Maksimov

“…However, it is precisely this that causes the massive prevalence of alcohol-related physical and mental health problems, as well as social functioning. The situation can be improved, including through early detection and effective behavioral counseling, especially in primary health care. …”
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Multivariate hypergeometric solutions of cosmological (dS) correlators by d log-form differential equations by Jiaqi Chen, Bo Feng, Yi-Xiao Tao

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Experimentally profiling dielectric properties of Escherichia coli and Staphylococcus aureus by movement velocity and force by Akmal Suhaimi, Arash Zulkarnain, Noraziah Mohamad Zin, Abdullah Abdulhameed, Aminuddin Ahmad Kayani, Ramdzan Buyong

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CPTU-Based Offshore Wind Monopile Rigid Bearing Mechanism Analysis by Jie Zhou, Renjie Gu, Panpan Shen, Chengjun Liu, Zeyao Li, Kefan Zhu, Zhenming Shi

“…A self-compiling parameter function is applied to soil modeling and an innovative mobile boundary function is created to simulate CPTU penetration. …”
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Partial Analysis of the Capsid Protein (VP1) of Human Sapovirus Isolated from Children with Diarrhoea in Rural Communities of South Africa by Mpho Magwalivha, Jean-Pierre Kabue Ngandu, Afsatou Ndama Traore, Natasha Potgieter

“…Twenty-five samples were randomly selected from 40 sapovirus-positive samples previously detected and analyzed for the VP1 region using the One-Step RT-PCR assay. …”
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Design, Modeling, and Control of a Soft Robotic Diaphragm‐Assist Device in a Respiratory Simulator by Diego Quevedo‐Moreno, Sang‐Yoep Lee, Jonathan Tagoe, Vishnu Emani, Jean Bonnemain, Ellen T. Roche

“…The device uses fabric‐based pneumatic actuators to provide targeted mechanical assistance during inhalation. A two‐step control system is implemented to optimize synchronization and support: 1) detecting breath intention from the pleural pressure signal to trigger the device and 2) regulating the device's input pressure to assist in inhalation. …”
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Beyond Global Metrics: The U-Smile Method for Explainable, Interpretable, and Transparent Variable Selection in Risk Prediction Models by Katarzyna B. Kubiak, Agata Konieczna, Anna Tyranska-Fobke, Barbara Więckowska

“…Variable selection (VS) is a critical step in developing predictive binary classification (BC) models. …”
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SentemQC - A novel and cost-efficient method for quality assurance and quality control of high-resolution frequency sensor data in fresh waters [version 2; peer review: 1 approved,... by Sofie Gyritia Madsen van't Veen, Joachim Audet, Eti Ester Levi, Brian Kronvang, Søren Erik Larsen, Erik Jeppesen, Esben Astrup Kristensen, Anders Nielsen, Thomas Alexander Davidson, Jane Rosenstand Laugesen, Peter Mejlhede Andersen

“…The SentemQC method is computationally efficient and features a six-step user-friendly setup for anomaly detection. The method marks anomalies in data using five moving windows. …”
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Hsa-miRNA-16-5p Of Urinary Exosomes A Reliable Biosignature Effective For Prostate Cancer Screening by Weng WH, Chiou YE, Zeng WZ, Chen MH, Pang ST

“…ROC analysis confirmed miR-16’s diagnostic potential, particularly in detecting mid-stage PCa.Conclusion: The proposed extraction method matches commercial kits in performance but offers notable advantages: a simplified five-step process, reduced extraction time (1.08 hours), and low cost ($0.13/test). …”
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A 56-year-old Patient with Recurrent Refractory Ascites and Abdominal Pain by K. S. Zemlyanukhina, I. N. Tikhonov, M. S. Zharkova, L. M. Tulina, M. A. Tsai, A. S. Tertychnyy, D. A. Svetov, A. S. Tyan

“…Laboratory signs of systemic inflammation were noted, while liver function remained intact. Laparoscopy and therapeutic and diagnostic laparocentesis were performed repeatedly — the ascitic fluid was an exudate (serum-ascitic albumin gradient — dance of deformed mesothelial cells was also noted. …”
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CD248 induces PD-L1 expression on cancer-associated fibroblasts to promote NSCLC immune escape by Zeyang Yang, Xuanyin Wang, Xu Zhu, Xu Zhu, Long Li, Long Li, Xianling Zeng, Jiaming Ren, Jiaming Ren, Lu Wang, Jiangwei Wu, Qiaoling Zhang, Siyu Wang, Maoqin Lu, Juan Zhai, Xinlei Liu, Jing Xiao, Tao Jin, Ying Zhang, Yun Wang, Yun Wang, Jian Zhang, Zhu Zeng, Zhu Zeng, Zhu Zeng, Jieheng Wu, Jieheng Wu, Jieheng Wu, Jieheng Wu, Jieheng Wu

“…BackgroundTumor immune escape is a critical step in tumor progression. Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) express abundant PD-L1 and suppress the functions of CD8+T cells, enablingd immune escape. …”
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Multi-material 3D printed smart floor tiles with triboelectric energy generation and security monitoring by Shidhin Mappoli, Kalyan Ghosh, Martin Pumera

“…However, the fabrication of different TENG layers using conventional techniques is often complex, time-intensive, and involves multiple processing steps. Here, a single-step multi-material 3D printing (MMP) approach is used to fabricate the fully functional TENG device, consisting of positive and negative triboelectric layers, current collectors and supporting substrate. …”
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Cosmetic crimes in dromedary camels: a critical overview on these illegal manipulations and its hidden consequences by Mohamed Tharwat

“…Visual assessment remains the primary diagnostic step, followed by imaging techniques (e.g., ultrasonography, radiography, thermography) and hormonal assays. …”
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HR eye & MMR eye: one-day assessment of DNA repair-defective tumors eligible for targeted therapy by Shinta Saito, Shingo Kato, Usaki Arai, Atsuki En, Jun Tsunezumi, Taichi Mizushima, Kensuke Tateishi, Noritaka Adachi

“…Overall, the findings presented here could pave the way for accurately assessing malignant tumors with functional defects in HR or MMR, a step forward in accelerating precision medicine.…”
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THE RELIABILITY OF DETERMINING THE SHORT CIRCUIT ZONE OF THE LINES OF 6–35 kV by F. А. Romaniuk, E. V. Buloichyk, O. A. Huryanchyk, V. S. Kachenya

“…It is shown that the application of the proposed correction method makes it possible to increase the accuracy of fault zone detection. The dynamic properties of the proposed method applied to different modes of the line functioning have been studied. …”
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Bird Call Identification Using Ensemble Empirical Mode Decomposition by Jingxuan Liu, Hailan Chen

“…In contrast to conventional approaches that are easily influenced by noise disruption and tend to encounter mode aliasing issues, the suggested framework proficiently segregates the signal by the following steps. First, the vocalization signals of bird vocalizations are preprocessed and endpoint detection is used to separate the vocalization signals of each call. …”
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Big cats (Carnivora, Felidae) still walking in Sierra de las Minas Biosphere Reserve: a forgotten Jaguar Conservation Unit in northeastern Guatemala by Luis A. Trujillo, Alejandro Mármol-Kattán, Cristina Abugarade, Adriana Rivera-Sandoval, Cristina Chaluleu-Baeza, Raquel Leonardo, Diana Mansilla, Allison Trinidad, Claudia Quijivix, Isabel Martínez, Cristina Escobar, Rocío Paz, Andrid Ramírez, Bárbara I. Escobar-Anleu, Rebeca Escobar, Rony Garcia-Anleu

“…The detection of Jaguars, Panthera onca (Linnaeus, 1758), and Pumas, Puma concolor (Linnaeus, 1771), in the Sierra de las Minas Biosphere Reserve, northeastern Guatemala, represents a pivotal step in understanding the distributional range of these apex predators in the country. …”
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