An optimized method for directed differentiation of hypothalamic neural stem cells in a 3D culture system by Jiao Luo, Qiaoyan Tang, Tanjing Lin, Jiabang Liu, Zhiheng Wu, Xintao Zhang, Xiaohua Zhang, Junhai Jiang, Yulong Wang

“…In this study, we present an optimized protocol for isolating and culturing hypothalamic neural stem cells (htNSCs) from neonatal (P1) mice, followed by their directed differentiation in a three-dimensional (3D) Matrigel environment. …”
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Systematic optimization of prime editing for enhanced efficiency and versatility in genome engineering across diverse cell types by Huiling Mu, Huiling Mu, Yeyi Liu, Yeyi Liu, Yijia Chi, Yijia Chi, Fei Wang, Shuting Meng, Shuting Meng, Yi Zhang, Yi Zhang, Xunting Wang, Xunting Wang, Dongxin Zhao, Dongxin Zhao, Dongxin Zhao

“…To further assess its efficacy in challenging cell types, we validated our optimized system in human pluripotent stem cells (hPSCs) in both primed and naïve states, achieving substantial editing efficiencies of up to 50%. …”
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Unraveling the underlying mechanisms of cancer stem cells in therapeutic resistance for optimizing treatment strategies by Yunhan Tan, Siyuan Qin, Zhe Zhang, Yongen Liu, Li Zhou, Bowen Li, Edouard C. Nice, Yuanyuan Zhang, Jing Jing

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Optimizing Timing and Preparation for Allogeneic Hematopoietic Stem Cell Transplantation in Higher-Risk Myelodysplastic Syndromes by Geng L, Chen E, Ji Y, Liu H, Sun Z

“…Liangquan Geng,1,* Erling Chen,2,* Yanping Ji,3,* Huilan Liu,1 Zimin Sun1,2,4 1Department of Hematology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, People’s Republic of China; 2Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People’s Republic of China; 3Department of Hematology, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China; 4Blood and Cell Therapy Institute, Division of Life Sciences and Medicine, Anhui Provincial Key Laboratory of Blood Research and Applications, University of Science and Technology of China, Hefei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zimin Sun, Department of Hematology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, No. 17 Lujiang Road, Luyang District, Hefei, Anhui, 230001, People’s Republic of China, Email zmsun@ustc.edu.cnIntroduction: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only curative treatment for higher-risk myelodysplastic syndromes (MDS), but optimal timing and donor selection remain controversial.Methods: We conducted a retrospective analysis of 70 higher-risk MDS patients classified by the revised International Prognostic Scoring System (IPSS-R) undergoing allo-HSCT. …”
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Modeling of Nonlinear Systems: Method of Optimal Injections by Anatoli Torokhti, Pablo Soto-Quiros

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Human stem cell models to unravel brain cancer by Biren Dave, Jignesh Tailor

“…In this review article, we aim to highlight the utility and promises of human stem cell-based models of brain tumors as a complementary system to traditional transgenic animal and cell line systems.…”
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Exploring the combined effect of optimally controlled chemo-stem cell therapy on a fractional-order cancer model. by Kaushik Dehingia, Sana Abdulkream Alharbi, Awatif Jahman Alqarni, Mounirah Areshi, Mona Alsulami, Reima Daher Alsemiry, Reem Allogmany, Homan Emadifar, Mati Ur Rahman

“…The Ulam-Hyers stability of the system is investigated. An optimal treatment schedule is developed to obtain the combined effect of chemotherapy and stem cell therapy on the model. …”
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Maintaining rockfall protection in mountain forests under climate change: optimizing management for sustainable stem size distributions by Ulrike Hiltner, Jonas Glatthorn, Timothy Thrippleton, Harald Bugmann

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An efficient propagation system through stem cuttings of a multipurpose plant—Ficus tikoua Bur by Tinghong Tan, Yu Peng, Biling An, Fan Gao, Yanni Sun, Chuandong Yang, Hong Yang, Zhihong Lu

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Clinical Progress in Mesenchymal Stem Cell Therapy: A Focus on Rheumatic Diseases by Helal F. Hetta, Alaa Elsaghir, Victor Coll Sijercic, Abdulrahman K. Ahmed, Sayed A. Gad, Mahlet S. Zeleke, Fawaz E. Alanazi, Yasmin N. Ramadan

“…ABSTRACT Background Rheumatic diseases are chronic immune‐mediated disorders affecting multiple organ systems and significantly impairing patients' quality of life. …”
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Overexpression of Decorin Optimizes the Treatment Efficacy of Umbilical Cord Mesenchymal Stem Cells in Bleomycin-Induced Pulmonary Fibrosis in Rats by Yaofeng Zhi, Minghui Shu, Pinsheng Tang, Yingjie Li, Min Guo, Jiongrui Deng, Haixin Mo, Meimei Wu, Baoyi Liu, Yanyang Mai, Jie Ling, Xulin Zhao, Xin Zhang, Wanli Zuo

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The Role of Mesenchymal Stem Cells in Modulating Adaptive Immune Responses in Multiple Sclerosis by Sepehr Dadfar, Esmaeil Yazdanpanah, Alireza Pazoki, Mohammad Hossein Nemati, Majid Eslami, Dariush Haghmorad, Valentyn Oksenych

“…Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system, leading to significant disability through neurodegeneration. …”
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Autologous hematopoietic stem-cell transplantation in diffuse cutaneous systemic sclerosis: a single-center experience by Marko Barešić, Ljiljana Smiljanić Tomičević, Igor Aurer, Mislav Cerovec, Branimir Anić

“… Systemic sclerosis (SSc) is a chronic autoimmune disorder characterized by multisystem involvement. …”
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Advances in genetically modified neural stem cell therapy for central nervous system injury and neurological diseases by Xiangwen Tang, Peng Deng, Lin Li, Yuqing He, Jinchao Wang, Dingjun Hao, Hao Yang

“…Abstract Neural stem cells (NSCs) have increasingly been recognized as the most promising candidates for cell-based therapies for the central nervous system (CNS) injuries, primarily due to their pluripotent differentiation capabilities, as well as their remarkable secretory and homing properties. …”
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Optimization of In-Situ Exosome Enrichment Methodology On-a-Chip to Mimic Tumor Microenvironment Induces Cancer Stemness in Glioblastoma Tumor Model by Saleheh Saffar, Ali Ghiaseddin, Shiva Irani, Amir Ali Hamidieh

“…Notch1 concentration tripled in the µBR system, correlating with enhanced stemness and phenotypic heterogeneity. …”
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Transdisciplinary integration of knowledge in the environment of a virtual stem center by Viktor Shapovalov, Oleksandr Stryzhak

“…The process of optimizing work processes in the modular system of the virtual STEM center is also considered. …”
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Transdisciplinary integration of knowledge in the environment of a virtual stem center by Віктор Шаповалов, Олександр Стрижак

“…The process of optimizing work processes in the modular system of the virtual STEM center is also considered. …”
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Potential of Stem-Cell-Induced Peripheral Nerve Regeneration: From Animal Models to Clinical Trials by Taylor M. Wynne, Virginia Grey Fritz, Zachary T. Simmons, Malek Zahed, Ananya Seth, Tamir Abbasi, Michael J. Reymundi, Kelly C. S. Roballo

“…This review is oriented to outline the potential role of stem cell therapies in peripheral nerve injury versus the current standards of care, compare the benefits and drawbacks of specific stem cell lines under investigation, and highlight the current models of stem cell therapy in the peripheral nervous system, with the ultimate goal of narrowing down and optimizing the role and scope of stem cell therapy in peripheral nerve injury.…”
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Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells by Esmeralda Magro-Lopez, Esmeralda Magro-Lopez, Esmeralda Magro-Lopez, Elena Vazquez-Alejo, Elena Vazquez-Alejo, Elena Vazquez-Alejo, María de la Sierra Espinar-Buitrago, María de la Sierra Espinar-Buitrago, María de la Sierra Espinar-Buitrago, María Ángeles Muñoz-Fernández, María Ángeles Muñoz-Fernández, María Ángeles Muñoz-Fernández

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TREATMENT OF ADVANCED SYSTEMIC MASTOCYTOSIS WITH MIDOSTAURIN: PRACTICAL GUIDANCE FOR OPTIMAL THERAPY AND MANAGEMENT by Cristina Papayannidis, Vincenzo Federico, Luana Fianchi, Patrizia Pregno, Novella Pugliese, Alessandra Romano, Federica Irene Grifoni

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