Dietary emulsifier carboxymethylcellulose-induced gut dysbiosis and SCFA reduction aggravate acute pancreatitis through classical monocyte activation by Yongpu Feng, Wenjin Chen, Jiayu Chen, Fengyuan Sun, Fanyang Kong, Lei Li, Yating Zhao, Shouxin Wu, Zhaoshen Li, Yiqi Du, Xiangyu Kong

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A Two-Stage Optimization Method for Multi-Runway Departure Sequencing Based on Continuous-Time Markov Chain by Guan Lian, Yingzi Wu, Weizhen Luo, Wenyong Li, Yaping Zhang, Xiaoyue Zhang

“…Then, continuous-time Markov chains (CTMC) were employed to track aircraft state transitions in the taxiway queue, and a nested whale optimization algorithm was proposed to optimize both the pushback sequence and runway resource allocation. Results indicate that the proposed method reduced the average taxiway queue time by 55.58%, with delay reductions of up to 73.06%, offering significant cost savings and environmental benefits while improving flight punctuality. …”
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Clinical benefits of enhanced 3D-NEVERview sequence in MRI simulation for nasopharyngeal carcinoma patients received radiotherapy by Yuanyuan Li, Yin Zhang, Ran Tang, Anyan Gu, Zhenyu Pan, Guozi Yang, Yaotao Li, Yu Wu, Zhuocheng Li, Lixiang Yang, Zhitao Dai, Xingru Sun

“…Abstract Background and purpose To test whether the enhanced 3D-NEVERview (3D-NEVERview + C) sequence improves delineation accuracy and allows clinically meaningful dose reductions to the brachial plexus of nasopharyngeal carcinoma (NPC) with cervical lymph node metastasis in radiotherapy during MRI simulation. …”
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Impact of a structured food sequence and mobile health monitoring on gestational diabetes outcomes: a clinical trial by Ria Murugesan, Shubhashree Thiruselvam, Kakithakara Vajravelu Leela, Abhishek Satheesan, K. Geetha, Mohan Ram, Janardanan Kumar

“…Dietary management is crucial in controlling blood glucose, and recent evidence suggests that the order in which food is consumed (food sequencing) can improve glycemic control. Mobile health (mHealth) tools also offer promising support for healthier eating habits and glucose monitoring. …”
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Machine learning revealed giant thermal conductivity reduction by strong phonon localization in two-angle disordered twisted multilayer graphene by Jingwen Wang, Zheng Zhu, Tianran Jiang, Ke Chen

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Clinical utility of a rapid two-dimensional balanced steady-state free precession sequence with deep learning reconstruction by Katerina Eyre, Moezedin Javad Rafiee, Margherita Leo, Junjie Ma, Elizabeth Hillier, Negin Amini, Josephine Pressacco, Martin A. Janich, Xucheng Zhu, Matthias G. Friedrich, Michael Chetrit

“…In the larger cohort, this accelerated cine sequence was successfully performed in all participants and significantly reduced the acquisition time of cine images compared to the standard 2D method (reduction of 37% (5.98/16) p < 0.0001). …”
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Comparing subsampling strategies for metagenomic analysis in microbial studies using amplicon sequence variants versus operational taxonomic units. by Daniel Segura, Divya Sharma, Osvaldo Espin-Garcia

“…Due to the high cost of shotgun metagenomic sequencing (SM-seq), microbiome analyses can be done cost-effectively in two phases: Phase 1-sequencing of 16S ribosomal RNA, and Phase 2-SM-seq of an informative subsample. …”
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Identification of Pathogenic Mutation c.286dupA in TYR Gene in an Individual with Oculocutaneous Albinism Using Exome Sequencing by Parto Hekmatpou, Parnian Hekmatpou, Farideh Jalali Mashayekhi, Sahar Bayat, Milad Gholami

“…DNA extraction and whole exome sequencing were performed. After analyzing the exome sequencing data, the pathogenic mutation was identified. …”
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Differential impact of divalent metals on native elongating transcript sequencing (NET-seq) protocols for RNA polymerases I and II. by Abigail K Huffines, David A Schneider

“…Recently, several next-generation sequencing techniques have emerged to study transcription at single-nucleotide resolution in vivo. …”
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Next generation sequencing identified bio-active peptides: Evaluation of its in vitro, in vivo and in silico anti-inflammatory activities by Megha Gowri Thippeswamy, Rajeshwara Achur, Ravikumar Hemagirigowda, Manjunatha Daddayya, Thoyajakshi Ramasamudhra Siddaraju, Shridhar Mathad, Shridhar Malladi, Huda Wazzan, Mohammad A. Wazan, Ahmed H. Abduljabbar, Anish Khan, Nagaraju Shivaiah

“…Recent advances in bioinformatics and next-generation sequencing (NGS) offer extensive molecular data for studying an organism's transcriptome and whole genome. …”
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Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens by Nattapong Langsiri, Wieland Meyer, Laszlo Irinyi, Navaporn Worasilchai, Nuttapon Pombubpa, Thidathip Wongsurawat, Piroon Jenjaroenpun, J. Jennifer Luangsa-ard, Ariya Chindamporn

“…Evaluation of the developed DNA extraction protocol on simulated clinical hemocultures revealed that the obtained DNA sequences exceed 10 kb in length, enabling a highly efficient sequencing run with over 80% active pores. …”
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Combined biochemical profiling and DNA sequencing in the expanded newborn screening for inherited metabolic diseases: the experience in an Italian reference center by Simona Fecarotta, Lorenzo Vaccaro, Alessandra Verde, Marianna Alagia, Alessandro Rossi, Chiara Colantuono, Maria Teresa Cacciapuoti, Patrizia Annunziata, Sara Riccardo, Antonio Grimaldi, Tonya Fusco, Rosa De Santis, Fernando Barretta, Lucia Albano, Daniela Crisci, Fabiana Vallone, Antonietta Tarallo, Marcella Cesana, Nicola Brunetti-Pierri, Giulia Frisso, Margherita Ruoppolo, Davide Cacchiarelli, Giancarlo Parenti

“…Advanced molecular technologies point to genomic sequencing as an alternative and feasible strategy for the screening of genetic diseases, including IMDs. …”
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Single-cell RNA-sequencing highlights a curtailed NK cell function in convalescent COVID-19 pregnant women by Madhuri S. Salker, Nor Haslinda Abd Aziz, Natalia Carman Prodan, Zhiqi Yang, Aditya Kumar Lankapalli, Katrin Lazar, Mohamad Nasir Shafiee, Ersoy Kocak, Ersoy Kocak, Harivignesh Ganesan, Surya Sekhar Pal, Omer Khalid, Norhana Mohd Kasim, Aida Kalok, Norashikin Abdul Fuad, Alfred Lennart Bissinger, Tina Ganzenmueller, Thomas Iftner, Karl Oliver Kagan, Stephan Ossowski, Stephan Ossowski, Nicolas Casadei, Nicolas Casadei, Sara Y. Brucker, Olaf Riess, Olaf Riess, Yogesh Singh, Yogesh Singh, Yogesh Singh, Deutsche COVID-19 OMICS Initiative (DeCOI)

“…We estimated cytokines and SARS-CoV-2 IgG antibodies in validation cohort using a multiplexd flow cytometry panel. Single-cell RNA sequencing (scRNA-seq) was performed using a Chromium Single Cell 3’ Gel Bead Chip and Library Kit from 10x Genomics (Drop-seq method). …”
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Enhancing bloodstream infection diagnostics: a novel filtration and targeted next-generation sequencing approach for precise pathogen identification by Ting-Syuan Lin, Ting-Syuan Lin, Ting-Syuan Lin, ZiHao Zhu, ZiHao Zhu, ZiHao Zhu, XiaoHong Lin, Hsi-Yuan Huang, Hsi-Yuan Huang, Hsi-Yuan Huang, Li-Ping Li, Li-Ping Li, Li-Ping Li, Jing Li, Jing Li, Jing Li, Jie Ni, Jie Ni, Jie Ni, PeiZhi Li, LanChun Chen, WeiXin Tang, HuiXin Liu, XiaoLong Se, MingFei Xie, Canling Long, Chih-Min Chiu, Szu-Han Fang, JiaMing Zhao, Yang-Chi-Dung Lin, Yang-Chi-Dung Lin, Yang-Chi-Dung Lin, XueTao Yu, Hsien-Da Huang, Hsien-Da Huang, Hsien-Da Huang, Hsien-Da Huang

“…Such delays and inaccuracies can critically impede timely clinical interventions, potentially compromising patient outcomes. Next-generation sequencing (NGS) is a powerful tool for rapid, precise pathogen identification. …”
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Improvement of protein quality and reduction of anti-nutritional factors in soybean meal by solid-state fermentation with Bacillus siamensis MH03 by Tanawut Chotinu-kul, Gunjana Theeragool, Duenrut Chonudomkul

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Responses of Methane Emission and Bacterial Community to Fertilizer Reduction Plus Organic Materials over the Course of an 85-Day Leaching Experiment by Jiakai Gao, Zhenyi Ma, Ling Liu, Zhaoyong Shi, Jialong Lv

“…In addition, high-throughput sequencing was utilized to investigate the alteration of the soil bacterial community structure. …”
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A high resolution genome-wide scan for significant selective sweeps: an application to pooled sequence data in laying chickens. by Saber Qanbari, Tim M Strom, Georg Haberer, Steffen Weigend, Almas A Gheyas, Frances Turner, David W Burt, Rudolf Preisinger, Daniel Gianola, Henner Simianer

“…We scanned the genome for local reductions in heterozygosity, suggestive of selective sweeps. …”
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Diversity, functionality, and stability: shaping ecosystem multifunctionality in the successional sequences of alpine meadows and alpine steppes on the Qinghai-Tibet Plateau by Xin Jin, Abby Deng, Yuejun Fan, Kun Ma, Yangan Zhao, Yingcheng Wang, Kaifu Zheng, Xueli Zhou, Guangxin Lu

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MET mutation causes muscular dysplasia and arthrogryposis by Hang Zhou, Chengjie Lian, Tingting Wang, Xiaoming Yang, Caixia Xu, Deying Su, Shuhui Zheng, Xiangyu Huang, Zhiheng Liao, Taifeng Zhou, Xianjian Qiu, Yuyu Chen, Bo Gao, Yongyong Li, Xudong Wang, Guoling You, Qihua Fu, Christina Gurnett, Dongsheng Huang, Peiqiang Su

“…By using whole‐exome sequencing, we confirmed MET p.Y1234C mutation to be responsible for arthrogryposis in this pedigree. …”
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Evaluating and prioritizing interdependent strategies for construction land reduction in China with interdependent sustainability criteria: an integrated multi-criteria decision-ma... by Jia-He Zhou, Chao-Yue Yu, Zheng-Feng Zhang, Lei He, Hong-Li Lin

“…Abstract Construction land reduction (CLR) represents China’s ambitious attempt to promote intensive and sustainable land use, yet its implementation faces challenges due to intricate interdependences within land use systems. …”
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