Genomic insights into tigecycline non-susceptibility in Clostridioides difficile: the role of the Tet P determinant and efflux mechanisms by Zijun Dang, Peiwen Xia, Jinzhu Huang, Bingxue Yang, Jiajia Liao, Qi Han, Yun Xia

“…Efflux pump involvement in resistance was evaluated using efflux pump inhibitors. Results Whole-genome sequencing revealed that C. difficile 16338 lacked plasmids and contained the Tet P determinant on its chromosome. …”
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Evolutionary characterization and pathogenicity of a porcine G9P[23] rotavirus with gene segments linked to canine and giant panda strains by Xi Li, Jingjing Wang, Yuankui Zhang, Yarong Zhao, Wenjun Liu, Yanli Shi

“…Therefore, evidence suggests interspecies transmission and genetic reassortment events between porcine, canine, and giant panda rotavirus strains. To evaluate the pathogenicity of ZJ03 strain, we experimentally infected 3-day-old piglets oral inoculation with the PoRV ZJ03 strain at a dose of 2 × 10^5.5 TCID50/ml per piglet. …”
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Dual oxic-anoxic co-culture enables direct study of anaerobe–host interactions at the airway epithelial interface by Patrick J. Moore, Kayla Hoffman, Sara Ahmed, Joshua R. Fletcher, Talia D. Wiggen, Sarah K. Lucas, Sabrina J. Arif, Adam J. Gilbertsen, Leslie A. Kent, Jessica K. Fiege, Ryan A. Langlois, Scott M. O'Grady, Ryan C. Hunter

“…We also combine DOAC with single-cell RNA sequencing to reveal a cell type-specific transcriptional response of the airway epithelium to F. nucleatum infection, including the increased expression of inflammatory marker genes and cancer-associated pathways. …”
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SV-MeCa: an XGBoost-based meta-caller approach for structural variant calling from short-read data by Rudel Christian Nkouamedjo Fankep, Arda Söylev, Anna-Lena Kobiela, Jochen Blom, Corinna Ernst, Susanne Motameny

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Soil Microbial Community Characteristics and Influencing Factors in Alpine Marsh Wetlands with Different Degradation Levels in Qilian Mountain National Park, Qinghai, China by Jintao Zhang, Xufeng Mao, Hongyan Yu, Xin Jin, Lele Zhang, Kai Du, Yanxiang Jin, Yongxiao Yang, Xianying Wang

“…The microbial community is one of the key indicators for evaluating the health of alpine marsh wetlands, and understanding the composition and health of alpine wetland communities provides a scientific rationale for conservation and restoration efforts. …”
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Isolation and Identification of <i>Burkholderia stagnalis</i> YJ-2 from the Rhizosphere Soil of <i>Woodsia ilvensis</i> to Explore Its Potential as a Biocontrol Agent Against Plant... by Xufei Zhu, Wanqing Ning, Wei Xiao, Zhaoren Wang, Shengli Li, Jinlong Zhang, Min Ren, Chengnan Xu, Bo Liu, Yanfeng Wang, Juanli Cheng, Jinshui Lin

“…Furthermore, various formulations of microbial agents based on <i>B. stagnalis</i> YJ-2 were evaluated for their efficacy against plant pathogens. …”
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Evaluating the utility of deep genome skimming for phylogenomic analyses: A case study in the species-rich genus Rhododendron by Zhi-Qiong Mo, Chao-Nan Fu, Alex D. Twyford, Pete M. Hollingsworth, Ting Zhang, Jun-Bo Yang, De-Zhu Li, Lian-Ming Gao

“…We first investigated locus recovery variation with sequencing depth, before evaluating the phylogenetic utility of different sets of loci, including Angiosperms353, target nuclear exons, and extended exon-flanking regions. …”
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Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR. by Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekinge

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A molecular dynamics-based algorithm for evaluating the glycosaminoglycan mimicking potential of synthetic, homogenous, sulfated small molecules. by Balaji Nagarajan, Nehru Viji Sankaranarayanan, Bhaumik B Patel, Umesh R Desai

“…Despite this multiplicity of function, natural GAG sequences have not yielded drugs because of problems of heterogeneity and synthesis. …”
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Establishment and Performance Evaluation of Multiplex PCR-Dipstick DNA Chromatography for Mycoplasma pneumoniae and Chlamydia pneumoniae Rapid Detection by Liuyang Hu, Xiuri Wang, Donglin Cao, Qiuchen Cheng, Qiong Li

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A PHYLOGENETIC APPROACH TO EVALUATE THE RELATION BETWEEN miRNA EXPRESSIONS AND HEPATITIS C VIRUS IN HEPATOCELLULAR CARCINOMA by Ayşe Banu Demir

“…Multiple alignment and phylogenetic analysis were used on stem loop sequences of miRNAs with significant expression changes and tried to identify a possible evolutionary relation of these miRNAs with HCV genotypes. …”
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Evaluating postmortem tongue fluids as a tool for monitoring PRRSV and IAV in the post-wean phases of swine production by Onyekachukwu Henry Osemeke, Isadora Machado, Mafalda Mil-Homens, Grant Allison, Michael Paustian, Daniel C. L. Linhares, Gustavo S. Silva

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Metagenomic evaluation of food hygiene practices in the National School Nutrition Programme in KwaZulu Natal, South Africa by Sithembile S. Madlala, Nokuthula Mchunu, Monica Dalasile, Rian Pierneef, Poovendhree Reddy

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Virtual screening and evaluation of bioactive peptides from Haliotis discus hannai as potential HMGCR inhibitors for hyperlipidemia treatment by Kun Qiao, Lina Liu, Yihui Chen, Qiongmei Huang, Bei Chen, Jingna Wu, Wenmei Huang, Zhiyu Liu

“…The inhibitory effects of these peptides on HMGCR activity were evaluated in vitro using a high-fat Hep-G2 cell model. …”
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Evaluating the Contribution of <i>Sporosarcina</i> to Carbonate Precipitation in Anaerobic Soils: A Microbial Community and Quantitative Analysis by Zen-ichiro Kimura, Ko-shiro Kirihara, Saki Komoto, Wataru Sera, Ryota Kojima, Sota Ihara, Yuya Itoiri, Daisuke Tanikawa, Yuki Iwasaki

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Comprehensive RNA-Seq Profiling to Evaluate the Sheep Mammary Gland Transcriptome in Response to Experimental Mycoplasma agalactiae Infection. by Rohini Chopra-Dewasthaly, Melanie Korb, René Brunthaler, Reinhard Ertl

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Predicting Potential miRNA Targets for Antibody Glycosylation in CHO Cells Using a Simplified Evaluation Workflow by Patrick Schlossbauer, Florian Klingler, Friedemann Hesse

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Considerations in biodistribution evaluation of iPSC-derived cell therapy: A pancreatic islet cell case study by Miyu Nakayama, Yuu Moriya, Hikaru Ueno, Takeshi Watanabe, Hideki Hirabayashi, Syunsuke Yamamoto

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Evaluating denitrification performance and microbial com-munities in sorghum stalk-based systems: Optimal conditions for nitrate removal by Zhilong Wang, Jun Xie, Guangjun Wang, Zhifei Li, Kai Zhang, Yun Xia, Jingjing Tian, Hongyan Li, Wenping Xie, Wangbao Gong

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The role of genetic testing in evaluating surgical outcomes for pediatric focal cortical dysplasia associated with NPRL3 variant by Bei Li, Liang Liu, Jianyun Su, Shanshan Jia, Zhijing Wang, Xia Li, Siyu Zhao

“…The patient underwent left frontal epileptogenic focus resection at the age of 6, with pathological findings confirming FCD type 1b. Whole-exome sequencing of blood and brain tissue samples revealed NPRL3 gene variants. …”
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