Global prevalence of Mycobacterium massiliense strains with recombinant rpoB genes (Rec-Mas) horizontally transferred from Mycobacterium abscessus: two major types, dominant circul... by Dong Hyun Kim, Hyejun Seo, Sangkwon Jung, Bum-Joon Kim

“…Here, for the first time, using 1,786 M. abscessus genome sequences, we evaluated the global prevalence of M. abscessus strains subjected to rpoB HGT. …”
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Gene expression profiling of human umbilical vein endothelial cells overexpressing CELF2 as diagnostic targets in diabetes-induced erectile dysfunction by Daniyaer Nuerdebieke, Lizhong Yao, Lange Guo, Jiuzhi Li, Hongliang Jia, Yukui Nan

“…The effects of CELF2 overexpression on cell proliferation and angiogenesis were assessed via CCK-8 and angiogenesis assays. RNA sequencing was employed to evaluate the gene expression profiles and alternative splicing events regulated by CELF2. …”
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Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils by Osama M. Ghanem, Nehal Kamal, Alaa F. Said

“…The promising isolates were identified by sequencing of their16S rRNA as Arthrobacter globiformis (MW3) and Micrococcus luteus (AB3). …”
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Influence of Asymmetric Pulses in Spread Spectrum Systems by A. P. Papavasileiou, C. N. Capsalis

“…This paper examines the impact of asymmetric pulses in NRZ waveforms generated by maximum-length sequences and used in spread spectrum systems. The data asymmetry phenomenon is produced by differential propagation delays through logic circuits in the payloads. …”
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Biodegradation of plastics and pesticides by soil bacteria in Bangladesh: Insights into antibiotic resistance and potential therapeutic targets by Azrin Ahmed, Shahin Mahmud, Mst. Tania Khatun, Md. Firoz Ali, Mst Rony Akter, A.K.M. Mohiuddin

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The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy by Kumar Uddipto, Julie A. Quinlivan, George L. Mendz

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Integrative single-cell and exosomal multi-omics uncovers SCNN1A and EFNA1 as non-invasive biomarkers and drivers of ovarian cancer metastasis by Liping Tang, Dong Pang, Chengbang Wang, Jiali Lin, Shaohua Chen, Jiangchun Wu, Junqi Cui

“…We then applied ten machine learning algorithm to exosomal transcriptomic data to evaluate diagnostic performance and identify the optimal classifier. …”
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Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass by Jinsong Yang, Songsong Zhao, Wenbo Zhi, Tianyu Lu, Huahua Qiao, Wei Liu, Ying Dou, Haisheng Tan, Hanlin Zhou, Hanlin Zhou

“…The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. …”
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Artificial Intelligence for Organoids Multidimensional Assessment by Yulin Mo, Jian Wang, Huijian Yang, Long Bai, Ke Xu, Jiacan Su

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Comparison of virulence and resistance genes in Mannheimia haemolytica and Pasteurella multocida from dairy cattle with and without bovine respiratory disease by Adriana Garzon, Craig Miramontes, Bart C. Weimer, Rodrigo Profeta, Alejandro Hoyos-Jaramillo, Heather M. Fritz, Richard V. Pereira

“…The objectives of this project were to (i) characterize virulence factor (VF) and antimicrobial resistance (AMR) genes in M. haemolytica and P. multocida isolates from dairy cattle of different ages with and without BRD using whole-genome sequencing (WGS); (ii) evaluate associations between microbial genetic elements and animal disease status; and (iii) assess the accuracy of genome-based predictions for the antimicrobial resistance phenotype. …”
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Investigating dose-dependent effects of chemical compounds targeting rumen fermentation pathways using an in-vitro rumen fermentation system by Rajan Dhakal, André Luis Alves Neves, Rumakanta Sapkota, Prabhat Khanal, Lea Ellegaard-Jensen, Anne Winding, Hanne Helene Hansen

“…In this study, potential methanogenesis inhibitors or electron acceptors were evaluated for their effects on methane production, fermentation, and prokaryotic community composition. …”
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Extraction of ultrashort DNA molecules from herbarium specimens by Rafal M. Gutaker, Ella Reiter, Anja Furtwängler, Verena J. Schuenemann, Hernán A. Burbano

“…Here, we applied these improvements to DNA extraction protocols for herbarium specimens and evaluated extraction performance by shotgun sequencing, which allows an accurate estimation of the distribution of DNA fragment lengths. …”
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Transcriptomic signatures in response to antioxidants supplementation in Korean cattle beef, Hanwoo: a 7-month feeding study by Kangwook Lee, La Yoon Choi, Jun Sang Ahn, Jae Yong Song, Joong Kook Park, Suk Jun Yun, Jeong Heon Lee, Eui-Cheol Shin, Soo-Jin Yeom, Jiangchao Zhao, Tae Jin Cho, Nam Su Oh, Jeong-Oh Shin, Dahye Kim, Tae Gyun Kim, Hyung Taek Cho, Hyo Ri Shin, Young Jun Kim, Jae Kyeom Kim, Jae Kyeom Kim

“…Growth performance and carcass traits were evaluated, and liver transcriptomic changes were assessed using RNA sequencing.Results and discussionWhile no significant differences were observed in phenotypic traits such as weight gain and feed conversion ratio, transcriptomic analysis identified 641 differentially expressed genes between the CON and FEED groups. …”
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Changes in gut microbiota after gastric cancer surgery: a prospective longitudinal study by Yuhua He, Shilin Gao, Lili Jiang, Jie Yang

“…BackgroundThis study was designed to characterize gut microbiota changes of the patients with gastric cancer before and after the gastrectomy during their hospital staying periods.Methods16S ribosomal RNA (rRNA) gene sequencing was used to evaluate differences in gut microbiota among patients with gastric cancer before and after the gastrectomy by comparing gut microbiota α diversity, β diversity, and structure composition at different taxonomic levels.ResultsA total of 120 fecal specimens were collected from 60 patients. …”
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scHeteroNet: A Heterophily‐Aware Graph Neural Network for Accurate Cell Type Annotation and Novel Cell Detection by Jiacheng Liu, Xingyu Fan, Chunbin Gu, Yaodong Yang, Bian Wu, Guangyong Chen, Chang‐Yu Hsieh, Pheng‐Ann Heng

“…Abstract Single‐cell RNA sequencing (scRNA‐seq) has unveiled extensive cellular heterogeneity, yet precise cell type annotation and the identification of novel cell populations remain significant challenges. scHeteroNet, a novel graph neural network framework specifically designed to leverage heterophily in scRNA‐seq data, is presented. …”
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Identification, Antimicrobial Susceptibility and Clinical Significance of <i>Klebsiella variicola</i> Strains by Alicja Sękowska, Yulian Konechnyi, Andrés Carrazco-Montalvo

“…The aim of the study was to identify <i>K. variicola</i> strains, evaluate the susceptibility of strains to selected antimicrobials, and detect their resistance mechanisms to β-lactams. …”
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Exploring the c.406 C > T variant in TNNI3 gene: pathogenic insights into restrictive cardiomyopathy by Tannaz Masoumi, Hamed Hesami, Majid Maleki, Samira Kalayinia

“…Methods Whole exome sequencing (WES) was conducted on the proband, followed by Sanger sequencing for variant confirmation and familial segregation analysis. …”
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The Impact of Jujube Witches’ Broom Phytoplasma on the Community Structure of Endophytes in Jujube by Nian Wang, Mengli Wang, Ziming Jiang, Wenzhe Zhang, Ziyang You, Xueru Zhao, Jia Yao, Chenrui Gong, Assunta Bertaccini, Jidong Li

“…To investigate the impact of jujube witches’ broom phytoplasma on the endophyte populations in jujube, high-throughput sequencing was performed in healthy and JWB-infected orchard jujube trees and <i>in vitro</i> jujube shoots. …”
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Long-read detection of transposable element mobilization in the soma of hypomethylated Arabidopsis thaliana individuals by Andrea Movilli, Svitlana Sushko, Fernando A. Rabanal, Detlef Weigel

“…Conclusions Long-read sequencing reveals widespread TE transposition in the soma of A. thaliana hypomethylated mutants. …”
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A Rare Cause of Hypergonadotropic Hypogonadism: Transaldolase Deficiency in Two Siblings by Melek Yıldız, Zerrin Önal, Gözde Yeşil, Tuğçe Göksu Kabil, Güven Toksoy, Şükran Poyrazoğlu, Firdevs Baş, Özlem Durmaz, Feyza Darendeliler

“…The diagnosis of transaldolase deficiency should be kept in mind for these patients, and they must be evaluated for gonadal functions, especially during puberty.…”
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