Characterization and functional evaluation of goat PDX1 regulatory modules through comparative analysis of conserved interspecies homologs by Naeimeh Rezaei, Kianoush Dormiani, Abbas Kiani-Esfahani, Somayeh Mirdamadian, Mohsen Rahmani, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani

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Evaluating Second-Generation Deep Learning Technique for Noise Reduction in Myocardial T1-Mapping Magnetic Resonance Imaging by Shungo Sawamura, Shingo Kato, Naofumi Yasuda, Takumi Iwahashi, Takamasa Hirano, Taiga Kato, Daisuke Utsunomiya

“…This study aimed to evaluate the impact of SR-DLR on noise reduction and measurement consistency in myocardial T1 mapping. …”
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Identification of deleterious variants associated with male infertility genes in a cohort of idiopathic hypospermatogenesis patients by Nisha Sharma, Ashutosh Halder, Seema Kaushal, Manoj Kumar, Manish Jain

“…Comprehensive clinical history, blood samples, semen analysis parameters, and reproductive endocrine evaluation reports of 51 hypospermatogenesis patients were collected. …”
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Evaluation of mycoparasitic Trichoderma atroviride and entomopathogenic Aspergillus niger as potential bioinsecticides against the dengue vector, Aedes aegypti by A. V. Ranthilini C. Banduwardena, A. V. Ranthilini C. Banduwardena, B. Anushka N. Mendis, Kasun M. Thambugala, Kasun M. Thambugala, Kasun M. Thambugala, H. Sachini D. Fernando, Itthayakorn Promputtha, Itthayakorn Promputtha, Itthayakorn Promputtha

“…This study details the evaluation of mycoparasitic Trichoderma atroviride and entomopathogenic Aspergillus niger against pyrethroid-resistant and pyrethroid-susceptible Ae. aegypti populations.Materials and methodsMolecular identification of the isolated entomopathogenic fungal strains was done using ITS-rDNA sequence data. …”
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Production of Protein Hydrolysates from Cod Backbone Using Selected Enzymes: Evaluation of Antioxidative and Antimicrobial Activities of Hydrolysates by Dimitra Marinou, Charlotte Jacobsen, Davide Odelli, Krystalia Sarigiannidou, Ann-Dorit Moltke Sørensen

“…Selected hydrolysates showing activity were evaluated with respect to amino acid composition and molecular weight. …”
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Performance evaluation of the Streck ARM-DⓇ Kit, β-Lactamase for molecular detection of acquired β-lactamase genes by Brian B. Yoo, Norihisa Yamamoto, Justina Ilutsik Quintero, Maria Jose Machado, Sarah Sabour, Sara Blosser, Maria Karlsson, James Kamile Rasheed, Allison C. Brown

“…Results: Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. …”
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Scheduling and Evaluation of a Power-Concentrated EMU on a Conventional Intercity Railway Based on the Minimum Connection Time by Yinan Wang, Limin Xu, Xiao Yang, Jingjing Bao, Feng Lin, Yiwei Guo, Yixiang Yue

“…The optimal connection plan with 14 groups of different route division plans was evaluated by using the entropy weight–TOPSIS method, and the optimal plan was obtained in the form of a route division method with two groups of routes with three pairs of trains in each group. …”
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Diffusion-weighted magnetic resonance imaging in evaluating malignant lymph node invasion in patients with female genital neoplasms by Basta-Nikolić Marijana, Nikolić Dragan, Stojanović Sanja, Đurđević Srđan, Nikolić Olivera, Till Viktor

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Design and immunogenic evaluation of multi-epitope vaccines for colorectal cancer: insights from molecular dynamics and In-Vitro studies by Peiwei Sun, Luolin Wang, Zhong Liu, Zhenglei Xu

“…Cross-reactivity and binding affinities were evaluated through molecular docking. Two multi-epitope vaccine constructs were designed incorporating the CTL epitopes, GM-CSF and IL-2 adjuvants, and a PADRE sequence. …”
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Decoding mutational signatures in breast cancer: Insights from a multi-cohort study by Margaux Betz, Andréa Witz, Julie Dardare, Cassandra Michel, Vincent Massard, Romain Boidot, Pauline Gilson, Jean-Louis Merlin, Alexandre Harlé

“…Materials and methods: DNA from patients of the CICLADES cohort was extracted, sequenced, and custom filtering was applied to the resulting files. …”
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Analysis of a Series of 26 Cases With Prenatal Skeletal Dysplasia via Multiplatform Genetic Detection by Li‐min Cui, Hua‐ying Hu, Xiao‐mei Zhai, Ming‐fei Qi, Yan‐ming Liu, Cong‐ying Han, Jing Zhang, Ming Shen, Yu‐lan Xiang, Wen‐qi Chen, Kai Yang, Dong‐liang Zhang, Huan‐xia Xing

“…Sanger sequencing and fluorescent quantitative PCR (QF‐PCR) were used as validation methods. …”
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Development of a lytic Ralstonia phage cocktail and evaluation of its control efficacy against tobacco bacterial wilt by Haoxin He, Ke Yi, Lei Yang, Yongfeng Jing, Lifu Kang, Zhihao Gao, Dong Xiang, Ge Tan, Yunsheng Wang, Qian Liu, Lin Xie, Shiya Jiang, Tianbo Liu, Wu Chen

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Immunoinformatic-driven design and evaluation of multi-epitope mRNA vaccine targeting HIV-1 gp120 by Muhammad Zeeshan Ahmed, Muhammad Zeeshan Ahmed, Tazeen Rao, Zeeshan Mutahir, Sarfraz Ahmed, Najeeb Ullah, Suvash Chandra Ojha

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Preclinical evaluation of a fully-human, quadrivalent-hantavirus polyclonal antibody derived from non-human source. by Dr Jay Hooper, Dr Steve Kwilas, Dr Rebecca Brocato, Mr. Matthew Josleyn, Ms. Lucia Principe, Mr Joshua Shamblin, Dr. Hua Wu, Dr. Christoph Bausch, Dr. Tom Luke, Dr Priya Karmali, Mr. Jerel Vega, Dr Padmanabh Chivukaula, Dr. Eddie Sullivan

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Development of a sensitive disease-screening model using comprehensive circulating microRNA profiles in dogs: A pilot study by Kohei Omura, Kaori Ide, Masashi Takahashi, Yu Furusawa, Masanori Kobayashi, Yuichi Miyagawa, Aki Fujiwara-Igarashi, Takahiro Teshima, Yoshiaki Kubo, Akiko Yasuda, Karin Yoshida, Noriyuki Hayakawa, Masato Kobayashi, Yasuyuki Momoi

“…Among these models, the one with the highest performance was chosen for evaluation. The diagnostic model, based on the comprehensive circulating miRNA profile developed in this study, achieved an AUC score of 0.89, with a sensitivity of 85 % and a specificity of 88 % for the disease samples. …”
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Evaluation of drug-drug interaction between Suraxavir Marboxil (GP681) and itraconazole, and assessment of the impact of gene polymorphism by Mai Han, Gang Cui, Yan Zhao, Xianbo Zuo, Xiaoxue Wang, Xin Zhang, Na Mi, Jiangli Jin, Chunyan Xiao, Jing Wang, Wei Wu, Yajuan Li, Jintong Li

“…This study assesses the in vivo drug-drug interaction (DDI) potential between GP681 (including its major metabolite GP1707D07, a substrate of CYP3A4) and itraconazole in healthy Chinese subjects, along with the safety profiles during co-administration. Additionally, it evaluates the impact of CYP1A2, CYP2C19, and CYP3A4 gene polymorphisms on GP1707D07 metabolism.MethodsThe study enrolled twelve healthy adult subjects to receive the treatments consisting of GP681 monotherapy and GP681-itraconazole co-administration in a fixed-sequence. …”
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The nonlinear viscoelasticity and its asymmetry of UV-cured resin under large amplitude oscillatory axial (LAOA) loading by Qiang Zhang, Yangguang Xu, Rui Xiao, Xiangyu Li

“…Further, the global asymmetry ratios of nonlinear viscoelasticity under LAOA loading are developed based on the sequence of physical process (SPP) approach throughout the entire oscillatory cycle. …”
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VenusMutHub: A systematic evaluation of protein mutation effect predictors on small-scale experimental data by Liang Zhang, Hua Pang, Chenghao Zhang, Song Li, Yang Tan, Fan Jiang, Mingchen Li, Yuanxi Yu, Ziyi Zhou, Banghao Wu, Bingxin Zhou, Hao Liu, Pan Tan, Liang Hong

“…These datasets feature direct biochemical measurements rather than surrogate readouts, providing a more rigorous assessment of model performance in predicting mutations that affect specific molecular functions. We evaluate 23 computational models across various methodological paradigms, such as sequence-based, structure-informed and evolutionary approaches. …”
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Comparison of MR Spectroscopy, 2-Point Dixon, and Multi-Echo T2* Sequences in Assessing Hepatic Fat Fraction Across a Diverse Range of Body Mass Index (BMI) and Waist Circumfer... by Brzeska B, Sabisz A, Kozak O, Szurowska E, Pieńkowska J

“…Beata Brzeska,1 Agnieszka Sabisz,1 Oliwia Kozak,2 Edyta Szurowska,1 Joanna Pieńkowska1 1II Department of Radiology, Faculty of Health Studies, Medical University of Gdansk, Gdansk, Poland; 2I Department of Radiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, PolandCorrespondence: Beata Brzeska, Email beabrzes@gumed.edu.plObjective: The study aimed to compare and evaluate the accuracy of three magnetic resonance imaging (MRI) sequences—MR liver spectroscopy, 2-point Dixon, and multi-echo T2*—in assessing hepatic fat fraction in patients with various body mass indexes (BMIs).Methods: 167 participants were recruited, including 110 healthy subjects with diverse BMIs and 57 bariatric surgery candidates. …”
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Validation of Core and Whole-Genome Multi-Locus Sequence Typing Schemes for Shiga-Toxin-Producing <i>E. coli</i> (STEC) Outbreak Detection in a National Surveillance Network, Pulse... by Molly M. Leeper, Morgan N. Schroeder, Taylor Griswold, Mohit Thakur, Krittika Krishnan, Lee S. Katz, Kelley B. Hise, Grant M. Williams, Steven G. Stroika, Sung B. Im, Rebecca L. Lindsey, Peyton A. Smith, Jasmine Huffman, Alyssa Kelley, Sara Cleland, Alan J. Collins, Shruti Gautam, Eishita Tyagi, Subin Park, João A. Carriço, Miguel P. Machado, Hannes Pouseele, Dolf Michielsen, Heather A. Carleton

“…In this study, we applied three WGS-based subtyping methods, high quality single-nucleotide polymorphism (hqSNP) analysis, whole genome multi-locus sequence typing using chromosome-associated loci [wgMLST (chrom)], and core genome multi-locus sequence typing (cgMLST), to isolate sequences from 11 STEC outbreaks. …”
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