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Amylin inhibits gastric cancer progression by targeting CCN1 and affecting the PI3K/AKT signalling pathway
Published 2025-12-01“…The expression of amylin in GC and its clinical correlation were evaluated using 38 pairs of GC and healthy human clinical samples. …”
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1466
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Tuberculous meningitis diagnosis and treatment: classic approaches and high-throughput pathways
Published 2025-01-01“…This review focuses on nucleic acid-based methods, including Xpert Ultra, metagenomic next-generation sequencing (mNGS), and single-cell sequencing of whole brain Tissue, alongside the diagnostic potential of metabolomic and proteomic biomarkers. …”
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1468
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Assessing the Influence of Selected Permeabilization Methods on Lymphocyte Single-Cell Multi-Omics
Published 2025-02-01“…We designed this study to evaluate previously recommended minimal permeabilization and fixation methods on the quality and quantity of transcriptomics and proteomics data generated by the BD Rhapsody™ Single-Cell Analysis System. (3) Results: Our findings showed that high-throughput sequencing with advanced quality and read-out is required for the combination of multi-omics outcomes from a permeabilized single cell. …”
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1471
Application of Amplicon Metagenomics to Identify Fungal Pathogens in Formalin-Fixed Paraffin-Embedded Samples: Proof of Concept in Animals with Fungal Pathologies
Published 2025-02-01“…The identification of fungal pathogens in formalin-fixed paraffin-embedded (FFPE) tissues is an unmet need in human and animal medicine, and sequence-agnostic approaches are needed to identify emerging pathogens. …”
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1472
Transcriptional regulation of SlFRIGIDA-like improve antioxidant system in tomato under cold
Published 2025-08-01“…Moreover, through RNA-sequencing, we found that different pathways related to cold stress were upregulated in the transgenic lines, indicating that it can improve tomato response to cold stress. …”
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1473
Exploring the effects of dietary methionine supplementation on European seabass mucosal immune responses against Tenacibaculum maritimum
Published 2025-01-01“…However, after infection, fish fed higher methionine levels exhibited a dose-dependent decrease in the mRNA expression of some proinflammatory genes. Similarly, RNA sequencing analysis of skin tissue revealed an attenuated immune response in the MET2 group at 24 hours post-infection, with few proinflammatory genes upregulated, which intensified at 48 h, potentially due to advanced tissue colonization by T. maritimum. …”
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Virome profiling of Aedes albopictus across urban ecosystems in Guangdong reveals sex-specific diversity
Published 2025-07-01“…Mosquito pools underwent viral particle enrichment followed by DNA and RNA sequencing. Bioinformatic analyses were employed to characterize viral communities, evaluate alpha/beta diversity, and conduct phylogenetic reconstruction. …”
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1478
First study on molecular identification of Anaplasma ovis in sheep in southern Kazakhstan
Published 2025-01-01“…The amplification products from the 441 positive samples were sequenced using the Sanger sequencing method. Phylogenetic analysis of the obtained sequences was performed using the maximum likelihood model. …”
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1479
A Mouse Model of Polycystic Ovary Syndrome Established Through Subcutaneous Administration of Letrozole Sustained-Release Pellets and Hepatic Transcriptome Analysis
Published 2025-04-01“…The extent of macrophage infiltration in the liver was evaluated via F4/80 immunohistochemical staining, and hepatic fibrosis levels were observed by Masson's trichrome staining. …”
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High proportion of tuberculosis recent transmission in rural areas of Northeastern China: a 3-year prospective population-based genotypic and spatial analysis in Hinggan League, Ch...
Published 2025-08-01“…We conducted a prospective study from 2021 to 2023, sequencing 221 MTB isolates from HL. After quality control, 210 cases were analyzed. …”
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