Comparative evaluation of sequencing platforms: Pacific Biosciences, Oxford Nanopore Technologies, and Illumina for 16S rRNA-based soil microbiome profiling by Vladimir Veselovsky, Mikhail Romanov, Polina Zoruk, Andrey Larin, Vladislav Babenko, Maxim Morozov, Aleksandra Strokach, Natalia Zakharevich, Sadokatkhon Khamidova, Albina Danilova, Aleksey Vatlin, Vsevolod Pavshintsev, Feng Chenguang, Denis Tsybizov, Nikita Mitkin, Olesya Galanova, Alexander Zakharenko, Kirill Golokhvast, Kirill Golokhvast, Ksenia Klimina

“…These results provide a comprehensive evaluation of sequencing platform performance.…”
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Development and evaluation of a deep learning framework for pelvic and sacral tumor segmentation from multi-sequence MRI: a retrospective study by Ping Yin, Weidao Chen, Qianrui Fan, Ruize Yu, Xia Liu, Tao Liu, Dawei Wang, Nan Hong

“…Abstract Background Accurate segmentation of pelvic and sacral tumors (PSTs) in multi-sequence magnetic resonance imaging (MRI) is essential for effective treatment and surgical planning. …”
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170. Evaluating The Impact of Early Mandibular Distraction Osteogenesis on Obstructive Sleep Apnea in Patients With Robin Sequence: A 20-year Perspective by Valeria Mejia, BS, Asli Pekcan, BS, Melanie Bakovic, BS, Raina K. Patel, BS, Marvee Turk, MD, Pasha Shakoori, DDS, MD, Mark M Urata, DDS, MD, Jeffrey A Hammoudeh, DDS, MD

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Is the Juice Worth the Squeeze? Evaluating Peptide Nucleic Acid Blockers for Reducing Plant DNA Contamination in 16S rRNA Gene Sequencing by Lena Flörl, Nicholas A. Bokulich

“…Next-generation sequencing of microbial communities frequently results in off-target amplification of the host or environmental DNA, which can lead to substantial data loss. …”
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P629: Analytical performance evaluation of whole genome sequencing as first-line genetic testing for intellectual development disorders and congenital malformations* by Peter Bui, Colyn Cain, Julie Cox, Mark Fleharty, Yueyao Gao, Benjamin Hilton, Sean Hofherr, Katie Larkin, Guang Li, David Marsh, Sarah South, Reha Toydemir

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Approaches to Evaluate Whole Exome Sequencing Data That Incorporate Genetic Intolerance Scores for Congenital Anomalies, Including Intronic Regions Adjacent to Exons by Kosuke Taniguchi, Fuyuki Hasegawa, Yuka Okazaki, Asuka Hori, Hiroko Ogata‐Kawata, Saki Aoto, Ohsuke Migita, Tomoko Kawai, Kazuhiko Nakabayashi, Kohji Okamura, Kana Fukui, Seiji Wada, Katsusuke Ozawa, Yushi Ito, Haruhiko Sago, Kenichiro Hata

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Evaluation of kefir consumption on gut microbial diversity in a healthy young population using full-length 16S rRNA sequencing by Yejin Choi, Gi Beom Keum, Juyoun Kang, Hyunok Doo, Jinok Kwak, Haram Kim, Yeongjae Chae, Suyoung Lee, Hyunjin Yang, Sheena Kim, Xingmin Sun, Hyeun Bum Kim, Soo Jin Yoo, Soo Jin Yoo

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Evaluating the Effect of Dietary Protein–Energy Ratios on Yak Intestinal Microbiota Using High-Throughput 16S rRNA Gene Sequencing by Yanbin Zhu, Yangji Cidan, Munwar Ali, Sijia Lu, Usama Javed, Zhuoma Cisang, Deji Gusang, Quzha Danzeng, Kun Li, Wangdui Basang

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Evaluation of different in silico tools for the assessment of deleterious variants in acute myeloid leukemia by Wardah Qureshi, Muhammad Irfan, Ishtiaq Ahmad Khan, Muhammad Shakeel

“…Several algorithms have been developed and evaluated to predict deleterious variants. The objective of this study was to assess the performance of 34 pathogenicity prediction tools (such as BayesDel, CADD, ClinPred, DANN, DEOGEN2, Eigen-PC, FATHMM, GERP++, M-CAP, MetaLR, MutationAssessor, MutationTaster, MutPred, Polyphen2, PROVEAN, REVEL, and SIFT) on the latest version of ClinVar dataset and implement it on the exome sequence data of acute myeloid leukemia (AML) patients to assess the performance to these tools in clinical samples. …”
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Splice_sim: a nucleotide conversion-enabled RNA-seq simulation and evaluation framework by Niko Popitsch, Tobias Neumann, Arndt von Haeseler, Stefan L. Ameres

“…Abstract Nucleotide conversion RNA sequencing techniques interrogate chemical RNA modifications in cellular transcripts, resulting in mismatch-containing reads. …”
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Appliance-Specific Noise-Aware Hyperparameter Tuning for Enhancing Non-Intrusive Load Monitoring Systems by João Góis, Lucas Pereira

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Improvement of Morkhor 60-3 Upland Rice Variety for Blast and Bacterial Blight Resistance Using Marker–Assisted Backcross Selection by Sawinee Panmaha, Chaiwat Netpakdee, Tanawat Wongsa, Sompong Chankaew, Tidarat Monkham, Jirawat Sanitchon

Subjects: “…simple sequence repeat…”
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Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses by Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal, Tony Brooks, Cristina Venturini, Charles Miller, Julianne R. Brown, Nathaniel Storey, Laura Atkinson, Timothy Best, Sunando Roy, Sian Goldsworthy, Sergi Castellano, Peter Simmonds, Heli Harvala, Tanya Golubchik, Rachel Williams, Judith Breuer, Sofia Morfopoulou, Oscar Enrique Torres Montaguth

“…Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories. …”
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Comparison of sequence- and structure-based antibody clustering approaches on simulated repertoire sequencing data. by Katharina Waury, Stefan Lelieveld, Sanne Abeln, Henk-Jan van den Ham

“…Repertoire sequencing allows us to investigate the antibody-mediated immune response. …”
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Evaluation of the efficacy of two methods for direct extraction of DNA from Mycobacterium tuberculosis sputum by Victor Ndhlovu, Wilson Mandala, Derek Sloan, Mercy Kamdolozi, Maxine Caws, Gerry Davies

“…We compared DNA extraction methods for direct extraction from Mycobacterium tuberculosis positive sputum and assessed their suitability for Single Molecule Real Time sequencing. Methodology: We evaluated the extraction efficiency of the PrimeXtract kit and an in-house CTAB method by extracting DNA from Mtb sputum. …”
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Evaluation of the genetic diversity and population structure of 5 pheasant breeds in Shanghai by Lina Qi, Xianyu Li, Jingle Jiang, Wengang Zhang, Xuelin Lu, Hongyan Yuan, Weijian Zhang

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Energy Stealth Evaluation of Radar Systems with a Space–Time Modulated Probing Signal by V. M. Kutuzov, V. P. Ipatov, S. S. Sokolov

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Evaluating stool microbiome integrity after domestic freezer storage using whole-metagenome sequencing, genome assembly, and antimicrobial resistance gene analysis by Paula Momo Cabrera, Nicholas A. Bokulich, Petra Zimmermann

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Development and Evaluation of a MinION Full-Length 16S rDNA Sequencing Analysis Pipeline for Rapid Diagnosis of Animal Gastrointestinal Diseases by Ying Zhong, Qingyun Pan, Yu Wang, Jinyan Yu, Yaomen Li, Lifang Gu, Meicun Hou, Shenglong Liang, Jia Guo, Xinan Jiao, Yunzeng Zhang

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[18F]-FLT-PET to evaluate how the sequencing of chemotherapies impacts the efficacy of combination treatment in mouse models of triple-negative breast cancer by Yun Lu, Jonathan Moye, Adriana V.F. Massicano, Carlos A. Gallegos, Shannon E Lynch, Patrick N. Song, Sharon Samuel, Anna G. Sorace

“…Introduction: Triple-negative breast cancer (TNBC) lacks targeted therapies due to an absence of biomarkers, making chemotherapy the primary treatment option for early-stage cancer. This study evaluates whether the order and sequence of combination chemotherapy—doxorubicin (DRB) and paclitaxel (PTX)—affects treatment efficacy in TNBC. …”
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