Construction of a novel CD8T cell-related index for predicting clinical outcomes and immune landscape in ovarian cancer by combined single-cell and RNA-sequencing analysis by Yu Zhang, Peng Wan, Liangliang Wang, Ruiping Ren

“…Methods The single-cell sequencing data from the EMTAB8107 cohort were used to identify CD8 T-cell subtypes. …”
Get full text

Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 (NGN3) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS) by Insung Park, Sergio Navarro-Serna, Raquel M. Pinho, Trish Berger, Elizabeth A. Maga, Joaquín Gadea, Seung K. Kim, Pablo J. Ross

“…Methods: Using a synthetic guide-RNA targeting porcine NGN3, we optimized the electroporation conditions to effectively introduce CRISPR/Cas9 in porcine zygotes and evaluate its effects on mosaicism and off-targets. …”
Get full text

Single-cell RNA sequencing reveals SLC31A1-mediated M2 polarization of macrophages promotes malignant progression in triple-negative breast cancer by Xiwei Zhang, Guoqing Li, Tieyan Chen, Haohang Sun, Ji Dai, Qi Chen, Mengze Chen, Meidi Yan

“…Copper ion and H2O2 detection kits were employed to evaluate the copper ion content and oxidative stress levels in macrophages. …”
Get full text

Detection of gene variants associated with recessive limb–girdle muscular weakness and Pompe disease in a global cohort of patients through the application of next-generation seque... by Jorge Alfredo Bevilacqua, Abdullah Mohammed Al-Salti, Abubaker Al Madani, Armando Alves da Fonseca, Hacer Durmus, Josiah Chai, Ali Alshehri, Márcia Gonçalves Ribeiro, Paulo Sgobbi, Sergey S. Nikitin, Steven Vargas, Adriana Furtado, Nathan Thibault, Roberto Araujo, Nadia Daba

“…Late-onset Pompe disease is an important disorder with a differential diagnosis for LGMD, where next-generation sequencing (NGS) plays a crucial role in accurate and prompt diagnosis. …”
Get full text

Integration of sequence data from a Consanguineous family with genetic data from an outbred population identifies PLB1 as a candidate rheumatoid arthritis risk gene. by Yukinori Okada, Dorothee Diogo, Jeffrey D Greenberg, Faten Mouassess, Walid A L Achkar, Robert S Fulton, Joshua C Denny, Namrata Gupta, Daniel Mirel, Stacy Gabriel, Gang Li, Joel M Kremer, Dimitrios A Pappas, Robert J Carroll, Anne E Eyler, Gosia Trynka, Eli A Stahl, Jing Cui, Richa Saxena, Marieke J H Coenen, Henk-Jan Guchelaar, Tom W J Huizinga, Philippe Dieudé, Xavier Mariette, Anne Barton, Helena Canhão, João E Fonseca, Niek de Vries, Paul P Tak, Larry W Moreland, S Louis Bridges, Corinne Miceli-Richard, Hyon K Choi, Yoichiro Kamatani, Pilar Galan, Mark Lathrop, Towfique Raj, Philip L De Jager, Soumya Raychaudhuri, Jane Worthington, Leonid Padyukov, Lars Klareskog, Katherine A Siminovitch, Peter K Gregersen, Elaine R Mardis, Thurayya Arayssi, Layla A Kazkaz, Robert M Plenge

“…We further evaluated PLB1 variants and risk of RA using a GWAS meta-analysis of 8,875 RA cases and 29,367 controls of European ancestry. …”
Get full text

Treatment sequences, outcomes, healthcare utilization, and costs in patients with inflammatory bowel diseases requiring advanced treatment—real world comparative effectiveness from... by Axel Dignass, Niels Teich, Stephan Kaiser, Juliane Sünwoldt, Christina Dünweber, Ines Weinhold, Julia Borchert, Robert Kudernatsch

“…Methods Evaluable patient characteristics, treatments, sequences, and outcomes were extracted from the WIG2 claims benchmark database and observed from 2014 to 2021. …”
Get full text

Follow-up of gestational trophoblastic disease/neoplasia via quantification of circulating nucleic acids of placental origin using C19MC microRNAs, hypermethylated , and sequences by Ilona Hromadnikova, Katerina Kotlabova, Ladislav Krofta, Filip Hron

“…The aim of the study was to evaluate the effectiveness of placental-specific markers, extracellular fetal DNA (sex-determining region Y and hypermethylated RASSF1A sequences) and circulating C19MC microRNAs (miR-516-5p, miR-517-5p, miR-518b, miR-520a-5p, miR-520h, miR-525, and miR-526a) for the diagnosis and consecutive follow-up of gestational trophoblastic disease/neoplasia. …”
Get full text

Molecular mechanisms of the Xiao-chai-hu-tang on chronic stress-induced colorectal cancer growth based on an integrated network pharmacology and RNA sequencing approach with experi... by Wang Yao, Dong-Ming Hua, Ying-Ru Zhang, Yi-Yang Zhao, Ying Feng, Zhao-Zhou Zhang, Zhong-Ya Ni, Hai-dong Guo, Yun-Feng Guan, Yan Wang

“…XCHT was administered via oral gavage once daily at dosages of 10.27 g/kg and 20.54 g/kg. RNA-sequencing was combined with network pharmacology to investigate potential target and pathway in this study. …”
Get full text

Discovering biomarkers associated with infiltration of CD8+ T cells and tumor-associated fibrosis in colon adenocarcinoma using single-cell RNA sequencing and gene co-expression ne... by Jinning Zhang, Jinning Zhang, Ziquan Sun, Ziquan Sun, Guodong Li, Guodong Li, Lixian Ding, Zitong Wang, Ming Liu, Ming Liu

Get full text

Mechanistic Insights into Shenzhuo Formula for Diabetic Retinopathy: Integrating UPLC-Q-TOF-MS/MS, Network Pharmacology, Single-Cell RNA Sequencing Data, and Experimental Validatio... by Zang X, Zhang L, Ma J, Wang A, Ding L, Wang Y, Sun J, Li J, Hang X, Li X, Zhao L

Get full text

Co-existence of plasmid-mediated blaNDM-1 and blaNDM-5 in Escherichia coli sequence type 167 and ST101 and their discrimination through restriction digestion by Amrita Bhattacharjee, Priyanka Basak, Shravani Mitra, Jagannath Sarkar, Shanta Dutta, Sulagna Basu

“…ABSTRACT The concurrent presence of multiple New Delhi metallo-β-lactamase (blaNDM) variants within an isolate often goes undetected without next-generation sequencing. This study detects and characterizes dual blaNDM variants in Escherichia coli through Sanger and whole-genome sequencing. …”
Get full text

Hybridized Brazilian–Bowein type spectral gradient projection method for constrained nonlinear equations by Jitsupa Deepho, Abdulkarim Hassan Ibrahim, Auwal Bala Abubakar, Maggie Aphane

Get full text

Low-hit frequency-hopping communication systems for power Internet of things random access by Shu DU, Mei MA, Bo ZHAO, Qi ZENG, Xing LIU

“…The communication network is an essential component of the data acquisition and information transmission in power Internet of things (IoT).To meet the development requirements of multiple power service in the future, the wireless communication technique with flexible-access and high scalability is one of the development directions for power IoT.Due to the features of large-scale access, random access time, high security and reliability for information transmission of power IoT, a frequency-hopping (FH) technique with low-hit rate for random access was proposed.The construction algorithm of such FH pattern was based upon the combination and shift operation of conventional FH sequences.By the theoretical arithmetic and numerical simulation, the properties of the proposed FH pattern and the error-rate of the FH-based power IoT were evaluated.The analysis reveals that the new class of FH sequences and the system can meet the requirements of large-scale access and highly reliable communication for the power IoT, which has good application prospects.…”
Get full text

Low-hit frequency-hopping communication systems for power Internet of things random access by Shu DU, Mei MA, Bo ZHAO, Qi ZENG, Xing LIU

“…The communication network is an essential component of the data acquisition and information transmission in power Internet of things (IoT).To meet the development requirements of multiple power service in the future, the wireless communication technique with flexible-access and high scalability is one of the development directions for power IoT.Due to the features of large-scale access, random access time, high security and reliability for information transmission of power IoT, a frequency-hopping (FH) technique with low-hit rate for random access was proposed.The construction algorithm of such FH pattern was based upon the combination and shift operation of conventional FH sequences.By the theoretical arithmetic and numerical simulation, the properties of the proposed FH pattern and the error-rate of the FH-based power IoT were evaluated.The analysis reveals that the new class of FH sequences and the system can meet the requirements of large-scale access and highly reliable communication for the power IoT, which has good application prospects.…”
Get full text

Improved Photosynthetic Accumulation Models for Biomass Estimation of Soybean and Cotton Using Vegetation Indices and Canopy Height by Jinglong Liu, Jordi J. Mallorqui, Albert Aguasca, Xavier Fàbregas, Antoni Broquetas, Jordi Llop, Mireia Mas, Feng Zhao, Yanan Wang

“…To further enhance the model performance and evaluate its applicability across different crop types, an improved PAM model (IPAM) is proposed with three strategies. …”
Get full text

Evaluation of DNA Extraction Methods for Microbial Community Profiling in Deadwood Decomposition by Yanmei Zhang, Zewei Song, Jonathan S. Schilling

Get full text

Assessment of Drought Tolerance in Barley Genotypes Through Phenotypical and Molecular Analysis by Mariam El Nabawy, Khadegah Najeeb, Khaled Abd El- Atey, Alia El-Seoudy

Get full text

A multimodal travel route recommendation system leveraging visual Transformers and self-attention mechanisms by Zhang Juan, Jing Zhang, Ming Gao

“…Concurrently, a Long Short-Term Memory (LSTM) network encodes sequential data to capture the temporal dependencies within travel sequences. To effectively merge these two modalities, a self-attention mechanism fuses the visual features and sequential encodings, thoroughly accounting for their interdependencies. …”
Get full text

Effects of reclaimed water irrigation and nitrogen fertilization on the chemical properties and microbial community of soil by Wei GUO, Mathias N Andersen, Xue-bin QI, Ping LI, Zhong-yang LI, Xiang-yang FAN, Yuan ZHOU

“…Based on a 16S ribosomal RNA (16S rRNA) sequence analysis, the Proteobacteria, Gemmatimonadetes and Bacteroidetes were more abundant in soil irrigated with reclaimed water than in soil irrigated with clean water. …”
Get full text

Probiotic potential and safety assessment of bacteriocinogenic Enterococcus faecalis CAUM157 by Arxel G. Elnar, Geun-Bae Kim

Get full text