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Comparative performance evaluation of FilmArray RP 2.1 and targeted next-generation sequencing in upper respiratory tract infections
Published 2025-07-01“…The implementation of accurate pathogen detection methods is crucial for precise diagnosis and effective clinical management of URTIs. This study evaluates the clinical performance of targeted next-generation sequencing (tNGS) as a diagnostic tool for comprehensive identification of URTI-associated pathogens.MethodsA total of 190 nasopharyngeal swab specimens from patients were enrolled for the study. …”
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Evaluating gene variations in autosomal dominant polycystic kidney disease patients using whole exome sequencing and phenotype to genotype analysis
Published 2025-12-01“…This study aimed to investigate novel variations in the PKD1, PKD2, and ADPKD-related genes through whole-exome sequencing (WES) and combine the genotype and phenotype data of the patients. …”
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A comprehensive evaluation of a novel targeted-sequencing workflow for Mycobacterium species identification and anti-tuberculosis drug-resistance detection
Published 2025-06-01“…BackgroundAlthough several World Health Organization–endorsed targeted next-generation sequencing (tNGS) assays exist for tuberculosis (TB) drug-resistance detection, their target selection and diagnostic accuracy vary widely. …”
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Development and evaluation of a haplotype reference panel for low-coverage whole genome sequencing genotype imputation in turbot (Scophthalmus maximus)
Published 2025-04-01“…As a core molecular breeding tool, whole genome sequencing (WGS) can improve the speed of selection and breeding of superior variety and promote the progress of biotechnology-based breeding. …”
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Transcriptomic Differences by RNA Sequencing for Evaluation of New Method for Long-Time In Vitro Culture of Cryopreserved Testicular Tissue for Oncologic Patients
Published 2024-09-01“…Objective: To test a new method for long-time in vitro culture of testicular fragments, thawed with two different regimes, with evaluation of transcriptomic differences by RNA sequencing. …”
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Evaluation of probiotic potential, safety assessment and whole genome sequencing of Lactiplantibacillus plantarum strain MOVIN isolated from toddy sample
Published 2025-07-01“…These isolates were also evaluated for probiotic traits such as auto-aggregation, hydrophobicity, tolerance to bile, acid, and NaCl. …”
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Evaluation of Different Sampling Methods Combined with Metagenomic Next-Generation Sequencing of Respiratory Specimens in Etiological Diagnosis of Patients with Severe Pneumonia
Published 2024-12-01“…Peng Zhou,1 Dehua Zhang,2 Jianjian Fu,3 Dongling Zhu,4 Shixiao Li5 1Department of Pharmacy, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 2Emergency Department, Enze Hospital, Taizhou Enze Medical Center (Group), Taizhou, Zhejiang, People’s Republic of China; 3Burn Department, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou, Zhejiang, People’s Republic of China; 4Nursing Department, Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, Taizhou, Zhejiang, People’s Republic of China; 5Department of Clinical Microbiology Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou, Zhejiang, People’s Republic of ChinaCorrespondence: Shixiao Li, Email lsx0992@126.comObjective: To evaluate the value of respiratory specimens collected via different sampling methods combined with metagenomic next-generation sequencing (mNGS) in the etiological diagnosis of severe pneumonia.Methods: A total of 117 patients with severe pneumonia between 2019 and 2024 were included in this study, with 60 patients undergoing endotracheal aspiration (ETA) and 57 undergoing bronchoalveolar lavage (BAL), respectively. …”
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Comparative diagnostic performance of metagenomic and two targeted sequencing methods in lower respiratory infection
Published 2025-07-01Subjects: “…Metagenomic next-generation sequencing…”
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A comprehensive evaluation of diversity measures for TCR repertoire profiling
Published 2025-05-01“…Using high-throughput sequencing, one can assess TCR repertoire in various clinical settings and describe it quantitatively by calculating a diversity index. …”
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Regional Economy Using Hybrid Sequence-to-Sequence-Based Deep Learning Approach
Published 2022-01-01“…Furthermore, in this study, to start developing the regional economy and make the revolution in this field to connect it with new technology, we train the deep learning algorithm of gathering factors to manage them perfectly and make a good prediction for the future economy. Hybrid sequence to sequence (seq2seq) algorithms of deep learning fed with previous information from past years and run the system to compare the predicted result data with current information to evaluate the method to be certified for the coming years.…”
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Implication of explicit knowledge and subjective evaluation in procedural perceptual-motor learning
Published 2025-04-01“…This study aims to investigate the roles of two individual factors related to learners’ strategies and mindsets: (a) explicit knowledge of the task’s characteristics and regularities and (b) subjective evaluation of the task and performance. We hypothesized that (a) participants reporting explicit knowledge of the task would exhibit higher PPML scores compared to those who did not, and (b) PPML scores would be related to subjective evaluation.MethodsParticipants were invited to practice two types of PPML tasks: motor sequence learning assessed by a Serial Reaction Time Task (SRTT) (Experiment 1) and visuomotor adaptation assessed by a Target Jumping Task (TJT) (Experiment 2). …”
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Evaluation of the effects of traditional irrigation solutions and etidronic acid on the bond strength of endodontic sealers
Published 2025-03-01“…This study aimed to evaluate the effect of ethylenediaminetetraacetic acid (EDTA) and Hydroxyethylidene Diphosphonic acid (HEDP) on bond strength of AH Plus and EndoSequence BC HiFlow sealers to root canal walls. …”
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