Exome sequencing shows same pattern of clonal tumor mutational burden, intratumor heterogenicity and clonal neoantigen between autologous tumor and Vigil product by David Willoughby, Ernest Bognar, Laura Stanbery, Casey Nagel, Gladice Wallraven, Aman Pruthi, Nicholas Bild, Ericca Stamper, Donald Rao, Adam Walter, John Nemunaitis

“…Evaluation of the pipeline using synthetic sequencing data from a sub-clonal deconvolution tool benchmarking study, showed positive predictive value (PPV) and positive percent agreement (PPA) of > 97.5% and > 96.5%, respectively, for SNV and InDel detection with minimum requirements for variant density and allele fraction. …”
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A first large study of whole-exome sequencing (WES) in 489 patients with suspected rare genetic disorders at a tertiary centre in Malaysia by Lip Hen Moey, Go Hun Seo, Boon Eu Cheah, Wee Teik Keng, Hane Lee, Gaik Siew Ch’ng

“…From August 2020 to December 2021, proband-only WES was offered to patients suspected of having a genetic disorder after clinical evaluation by a clinical geneticist. WES was performed at a single reference laboratory in South Korea. …”
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Human factors evaluation of an innovative wound care technology by Anna Bradford, Kathryn Arnold, Katherine VanTil, Jill de Grood, Kathryn Ambler, Patty Wickson, Chester Ho

“…Thematic analysis and journey mapping were conducted based on participant interviews to compare the user experience and satisfaction between NanoSALV and the current state from different patient and provider perspectives. Results: The evaluation indicated that the procedures for changing wound dressings, whether using the current state dressing or NanoSALV, followed the same sequence of steps, with NanoSALV requiring fewer subtasks in the application step, demonstrating the feasibility of implementing NanoSALV into clinical practice. …”
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The Evaluation of Tectonic Activity in Eshtehard Plain Basin with Interferometry by Farrin Nayebzadeh, Aghil Madadi, Ghasem Azizi

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Comparative relation mining of customer reviews based on a hybrid CSR method by Song Gao, Hongwei Wang, Yuanjun Zhu, Jiaqi Liu, Ou Tang

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Comparison of cleaning effectiveness of single rotary file OneShape and reciprocating F2 Protaper with Protaper Universal sequence: A SEM analysis by Adish A. Saraf, Anand C. Patil, T.M. Mangala, Rushikesh Mahaparale, Sneha Mali, Sagar Pawar

“…Intergroup comparisons revealed a statistically significant difference (p ≤ 0.05) in the middle and apical 1/3rd when the smear layer was evaluated. Conclusion: The Protaper full sequence group provided better results than Single F2 ProTaper and One shape groups when debris and smear layer removal was investigated.…”
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LPBERT: A Protein–Protein Interaction Prediction Method Based on a Pre-Trained Language Model by An Hu, Linai Kuang, Dinghai Yang

“…The prediction of protein–protein interactions is a key task in proteomics. Since protein sequences are easily available and understandable, they have become the primary data source for predicting protein–protein interactions. …”
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Detection and Localization of Rotor Winding Inter-Turn Short Circuit Fault in DFIG Using Zero-Sequence Current Component Under Variable Operating Conditions by Muhammad Shahzad Aziz, Jianzhong Zhang, Sarvarbek Ruzimov, Xu Huang

“…This paper presents a comprehensive methodology for online ITSC fault diagnosis in DFIG rotor windings based on zero-sequence current (ZSC) component analysis under variable operating conditions. …”
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Search for associations of polymorphic variants of the <i>MTHFR, MET, CHEK2</i> genes, identified through next-generation sequencing, with cervical cancer by K. V. Lenkova, R. M. Minyazeva, V. L. Akhmetova, I. R. Gilyazova, R. I. Khusainova, I. R. Minniakhmetov

“…Additionally, clinical significance of the identified substitutions was evaluated using literature data, databases and bioinformatics methods. …”
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Exploring the clinical outcomes and molecular characteristics of Acinetobacter baumannii bloodstream infections: a study of sequence types, capsular types, and drug resistance in C... by Jiao Chen, Yanting Shao, Zhibin Cheng, Guanghui Li, Fen Wan, Chenyan Gao, Danqin Wu, Dandan Wei, Dandan Wei, Yang Liu, Yang Liu, Rong Li

“…The clinical characteristics of all A. baumannii isolates were evaluated. Virulence phenotypes of all isolates were evaluated using the growth curve, biofilm-forming assay, antiserum complement killing, and G.mellonella killing assay. …”
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OperonSEQer: A set of machine-learning algorithms with threshold voting for detection of operon pairs using short-read RNA-sequencing data. by Raga Krishnakumar, Anne M Ruffing

“…We show that our approach detects operon pairs that are missed by current methods by comparing our predictions to publicly available long-read sequencing data. OperonSEQer therefore improves on existing methods in terms of accuracy, flexibility, and adaptability.…”
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Next-generation sequencing guides the treatment of severe community-acquired pneumonia with empiric antimicrobial therapy failure: A propensity-score-matched study. by Yuhao Zhao, Yajuan Wang, Yi Li, Chao Song, Pinhua Pan

“…<h4>Background</h4>Next-generation sequencing (NGS) is a promising diagnostic tool for pathogens diagnosis. …”
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Illuminating choices for library prep: a comparison of library preparation methods for whole genome sequencing of Cryptococcus neoformans using Illumina HiSeq. by Johanna Rhodes, Mathew A Beale, Matthew C Fisher

“…The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually. …”
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Comparative sequence, antigenic and phylogenetic analysis of avian influenza (H9N2) surface proteins isolated in Pakistan between 1999 and 2008 by Azeem Mehmood Butt, Samerene Siddique, Shifa Tahir, Izza Nasrullah, Mureed Hussain, Muhammad Idrees, Jun Lu, Yigang Tong

“…Conclusions: Because of the rapid mutating nature of avian influenza subtype H9N2, constant surveillance of annual sequence variations is important. Preventive measures and vaccine products can be evaluated by keeping track of changes that may lead to reassortment among different circulating strains in Pakistan's commercial poultry flocks or in humans.…”
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Prognostic Value of TBC1D1 and Its Relationship With the Tumor Microenvironment in Pancreatic Cancer: A Study Based on Single‐Cell Sequencing by Linfeng Yang, Duan Yan, Jun Yu, Dawei Deng, Lin Ma, Ruixin Yu, Song Wei, Jiahui Yu, Chuan Lan, Pengsheng Yi

“…In this study, we aimed to evaluate the prognostic value of TBC1D1 and its correlation with the tumor microenvironment (TME) in PC. …”
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Integrated analysis of single-cell and bulk RNA sequencing to construct a CD8+ T cell-related immune gene signature in pancreatic cancer by Yuzhi Liu, Fei Xu, Anyi Jiang, Jie Ding, Chungao Li, Ming Quan

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Nanopore-based targeted next-generation sequencing (tNGS): A versatile technology specialized in detecting low bacterial load clinical specimens. by Chen Yang, Weiwei Gao, Yicheng Guo, Yi Zeng

“…Nanopore-based targeted next-generation sequencing (tNGS) offers advantages such as convenience, efficiency, and long-read sequencing, making it a commonly used method for mycobacteria identification.…”
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Sensitivity of Diffusion-Weighted Image Combined with T2 Turbo Inversion Recovery Magnitude Sequence and as an Alternative to Contrast-Enhanced MRI in the Detection of Perianal Fis... by Noor Fadhil Baqir, Rasha Sabeeh Ahmed, Khaleel Ibraheem Mohsen

“…Diffusion-weighted images and T2 turbo inversion recovery magnitude sequences exhibit comparable efficacy to T1-enhanced contrast sequences in detecting perianal fistula. …”
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Integrating Nanopore MinION Sequencing into National Animal Health AMR Surveillance Programs: An Indonesian Pilot Study of Chicken Slaughterhouse Effluent and Rivers by Rallya Telussa, Puji Rahayu, Thufeil Yunindika, Curtis J. Kapsak, Kanti Puji Rahayu, Oli Susanti, Imron Suandy, Nuraini Triwijayanti, Aji B. Niasono, Syamsul Ma’arif, Hendra Wibawa, Lestari Lestari, Gunawan B. Utomo, Farida C. Zenal, Luuk Schoonman, Lee E. Voth-Gaeddert

“…<b>Conclusions:</b> Cost–benefit comparisons suggest sequencing can complement culture-based methods but is dependent on the value placed on the additional information gained.…”
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Genomic Analysis of Advanced Phyllodes Tumors Using Next-Generation Sequencing and Their Chemotherapy Response: A Retrospective Study Using the C-CAT Database by Shuhei Suzuki, Yosuke Saito

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