High-resolution melting of 12S rRNA and cytochrome b DNA sequences for discrimination of species within distinct European animal families. by Jana Naue, Tobias Hansmann, Ulrike Schmidt

“…The use of high-resolution melting (HRM) of DNA provides a fast closed-tube method for analysis of the sequence composition of the mitochondrial genes 12S rRNA and cytochrome b. …”
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Genome-wide high-throughput screening to investigate essential genes involved in methicillin-resistant Staphylococcus aureus Sequence Type 398 survival. by Mette T Christiansen, Rolf S Kaas, Roy R Chaudhuri, Mark A Holmes, Henrik Hasman, Frank M Aarestrup

“…Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) Sequence Type 398 (ST398) is an opportunistic pathogen that is able to colonize and cause disease in several animal species including humans. …”
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Community well-being service supply to the urban elderly based on preferred sequence demand: a case study in Zhenhai district, Ningbo by Siqi Shao, Tiantian Che, Yuxia Wu

“…This study applies the Kano model to identify the hierarchy of community-based service needs among urban elderly residents and determine a prioritized sequence of service provision to guide improvements in service supply. …”
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Differentiation of Pneumocystis jirovecii pneumonia from colonization: a clinical decision framework incorporating risk stratification and next-generation sequencing thresholds by Xiaoyan Sun, Peng Zhang, Kaiyu Zhang

“…When NGS results yield a P. jirovecii-specific sequence reads below the cutoff value of 37, a definitive diagnosis of PJP is unlikely. …”
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Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium <i>Paraliobacillus zengyii</i> CGMCC1.16464 by Qianjin Fan, Mengqi Jiao, Haoyue Huangfu, Lan Chen, Beijie Li, Zhijie Cao, Xuelian Luo, Jianguo Xu

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A clinically feasible algorithm for the parallel detection of glioma‐associated copy number variation markers based on shallow whole genome sequencing by Shuai Wu, Chenyu Ma, Jiawei Cai, Chenkang Yang, Xiaojia Liu, Chen Luo, Jingyi Yang, Zhang Xiong, Dandan Cao, Hong Chen

“…Shallow whole genome sequencing (sWGS) has been widely used for CNV detection due to its cost‐effectiveness and versatility. …”
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Deep immunoglobulin repertoire sequencing depicts a comprehensive atlas of spike-specific antibody lineages shared among COVID-19 convalescents by Qihong Yan, Yudi Zhang, Ruitian Hou, Wenjing Pan, Huan Liang, Xijie Gao, Weiqi Deng, Xiaohan Huang, Linbing Qu, Congli Tang, Ping He, Banghui Liu, Qian Wang, Xinwei Zhao, Zihan Lin, Zhaoming Chen, Pingchao Li, Jian Han, Xiaoli Xiong, Jincun Zhao, Song Li, Xuefeng Niu, Ling Chen

“…By clustering 2677 spike-specific antibodies with 360 million IgH sequences that we sequenced, a total of 329 shared spike-specific antibody clonotypes were identified from 33 COVID-19 convalescents and 24 SARS-CoV-2-naïve individuals. …”
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Prediction of favorable geological storage areas based on sedimentary characteristics: A case study on the northeastern Ordos Basin, China by Yue Zhao, Qiang Xu, Song Du, Yang Zhang, Tengzhuo Zhang, Yinglin Fan, Yan Ding, Sitong Song, Xinxin Feng

“…However, few studies have been made to evaluate the sedimentary and sequence stratigraphy characteristics of this region, as well as their influence on carbon dioxide and saline water storage potential. …”
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Laboratory Test Indirectly Reveals the Unreliability of RNA-Dependent 16S rRNA Amplicon Sequences in Detecting the Gut Bacterial Diversity of <i>Delia antiqua</i> by Miaomiao Li, Xin Cao, Linfeng Xu, Luyao Lin, Xiaoqing Wu, Susu Fan, Xinjian Zhang, Fangyuan Zhou

“…In contrast, RNA-dependent sequencing can identify alive bacterial communities, making them more suitable for evaluating alive microbiota diversity. …”
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SCAR marker linked to resistance gene of powdery mildew in bottle gourd [Lagenaria siceraria (Molina) Standl.] breeding line J083 by WANG Ling-ping, WU Xiao-hua, WANG Bao-gen, XU Pei, LI Guo-jing

“…The AFLP fragment was then converted to a sequence-characterized amplified region (SCAR) marker, named as GPDS_ATG/CTC75. …”
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Assessing the Leadership Qualities of Industrial Enterprises’ Managers: Methodological Approach by Andrey T. Kirichenko, Tatiana V. Yakovenko

“…The purpose of the article is to substantiate the methodological sequence of leadership assessment in the personnel management system of industrial enterprises, contributing to their effective operation. …”

Quantification of thermo-halotolerant alkaline protease activity derived from Bacillus licheniformis strains isolated from extreme environments in Morocco by Taha Chouati, Ounayssa Ayadi, Mohammed Ajdig, Lahcen Ouchari, Bahia Rached, Jamila El Alami, Elmostafa EL Fahime

“…Bacterial identification to the species level was performed using 16S rRNA gene sequencing and MultiLocus Sequence Typing (MLST), a technique that is much more precise and can identify the bacteria to the strain level. …”
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Genetic Approach to Target Putative Pks Genes in <i>Aspergillus</i> Section Nigri Species Producing Ochratoxin A by Sabah Ben Fredj Melki, Angelique Gautier, Ahmed Mliki

“…Phylogenetic methods were used to align and evaluate the sequences. The study’s primers demonstrated broad application, and several <i>Aspergillus</i> species from the section Nigri, particularly <i>A. niger</i> and <i>A. tubingensis</i>, were amplified satisfactorily. …”
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InSAR deformation observation and regional severe earthquake hazard analysis of the 2016 and 2022 strong earthquake activities in Menyuan, Qinghai by CHENG Yan, JIANG Yanan, HOU Zhongjian, ZENG Rui, LUO Huiyuan

“…Furthermore, the static Coulomb stress changes on the faults in the region and its surroundings caused by the Menyuan Mw 6.6 earthquake in 2022 were evaluated.   Results  The coseismic deformation field in 2016 exhibited a single elliptical uplift center, with predominantly thrust motion. …”
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Emergence of ST11-KL64 carbapenem-resistant hypervirulent Klebsiella Pneumoniae isolates harboring bla KPC−2 and iucA from a tertiary teaching hospital in Western China by Haojun Chen, Tingting Li, Xiaoxue Huang, Qiurong He

“…Molecular characteristics of CRKP included serotype, carbapenemases, virulence-associated factors, and multilocus sequence typing (MLST) performed by using the PCR method. …”
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The Effect of Cross-Section of Three Different Files on Apical Debris and Irrigant Solution Extrusion Using Full File Sequences: An In Vitro Study by Sohaib Fadhil Mohammed, Sura Yaseen Khudhur, Zaidoon Hasan Mohammed, Matheel AL-Rawas, Tahir Yusuf Noorani, Mohd Firdaus Yhaya

“…Objective: The aim of the study was to evaluate the cross-sectional geometry of heat-treated endodontic rotary files upon debris ejection through apex and the quantity of irrigants expelled when employing a complete sequence file system. …”
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Healthcare providers' knowledge, attitudes, and perceptions from using targeted sequencing to diagnose and manage drug-resistant tuberculosis (DR-TB) in Eswatini. by Maia Madison, Debrah Vambe, Sein Sein Thi, Mangaliso Ziyane, Nosisa Shiba, Babongile Blisset Nkala, Tara Ness, Agostinho Viana Lima, Sindisiwe Dlamini, Siphiwe Ngwenya, Anna Mandalakas, Alexander Kay

“…Design-locked Targeted Sequencing (TS) assays have the potential to improve DR-TB diagnosis and management. …”
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Analytical validation of the Percepta Nasal Swab classifier; an RNA next-generation sequencing assay for the assessment of lung cancer risk in pulmonary nodules by Shuyang Wu, Ruochen Jiang, Grazyna Fedorowicz, Mei Wong, Janna S. Chamberlin, Lori Lofaro, P. Sean Walsh, Giulia C. Kennedy, Yangyang Hao, Jing Huang, Bill Bulman

“…The PNS classifier leverages this concept using whole transcriptome sequencing (RNASeq) of cells collected from the nasal epithelium and provides “high”, “intermediate” and “low risk” classification calls to help guide clinical management decisions. …”
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Exploring the impact of cuproptosis on prostate cancer prognosis via RNA methylation regulation based on single cell and bulk RNA sequencing data by Junchao Wu, Junchao Wu, Junchao Wu, Wentian Wu, Jiaxuan Qin, Jiaxuan Qin, Jiaxuan Qin, Ziqi Chen, Ziqi Chen, Ziqi Chen, Rongfang Zhong, Peng Guo, Song Fan, Song Fan, Song Fan

“…We used receiver operating characteristic curves and C-index to evaluate the accuracy of our prognostic model. In conjunction with this, we conducted single-cell RNA sequencing (scRNA-seq) analyses to investigate underlying mechanisms and evaluate the degree of immune infiltration, as well as to assess patients’ responses to diverse chemotherapy agents. …”
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Cross-Activity Analysis of CRISPR/Cas9 Editing in Gene Families of <i>Solanum lycopersicum</i> Detected by Long-Read Sequencing by Ofri Kutchinsky, Dongqi Li, Guy Assa, Asaph Aharoni, Zohar Yakhini

“…CRISPR/Cas9 genome editing holds promise for precise genetic modifications, yet off-target effects remain a concern—particularly in gene families with high sequence similarity. In this study, we present a computational framework for analyzing editing specificity and cross-reactivity in gene families using long-read sequencing data. …”
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