Sialyltransferase gene signature as a predictor of prognosis and therapeutic response in kidney renal clear cell carcinoma by Huiyu Liu, Changwei Zhou, Zaichun Yan, Hairong Yang, Yun Zhao, Rui Tian, Xuejun Bo, Leizuo Zhao, Wei Ren

“…Validation cohorts of KIRC samples were obtained from the International Cancer Genome Consortium (ICGC) database. Single-cell RNA sequencing (scRNA-seq) data were downloaded from the Gene Expression Omnibus (GEO) platform, and preprocessing, normalization, and dimensionality reduction analyses were conducted using the "Seurat" package. …”
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Impact of increasing CD4 count threshold eligibility for antiretroviral therapy initiation on advanced HIV disease and tuberculosis prevalence and incidence in South Africa: an int... by Richard John Lessells, Kogieleum Naidoo, Nigel Garrett, Jienchi Dorward, Lara Lewis, Christian Bottomley, Yukteshwar Sookrajh, Kwabena Asare, Johan van der Molen, Thokozani Khubone, Thulani Ngwenya, Ntokozo Siyabonga Mkhize, Phelelani Sosibo

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Differential Responses to Targeted Therapies in Non-Small Cell Lung Cancer: A Comparative Analysis of Outcomes in Patients with Single EGFR Mutation and Concurrent Gene Alterations by Le LT, Nguyen NV, Trinh HL, Van Le Q, Dao THT, Nguyen Ngoc S, Van LD, Thi Nguyen T

“…Linh Tu Le,1,2 Nhung Viet Nguyen,3 Huy Le Trinh,4,5 Quang Van Le,4– 6 Trang Huyen Thi Dao,7,8 Son Nguyen Ngoc,7 Luong Dinh Van,1,2 Trang Thi Nguyen7– 10 1Department of Oncology, National Lung Hospital, Hanoi, 100000, Vietnam; 2Department of Tuberculosis and Lung Disease, Hanoi Medical University, Hanoi, 100000, Vietnam; 3Department of Pulmonology, University of Medicine and Pharmacy, Vietnam National University, Hanoi, 100000, Vietnam; 4Department of Oncology, Hanoi Medical University, Hanoi, 100000, Vietnam; 5Department of Oncology and Palliative Care, Hanoi Medical University Hospital, Hanoi Medical University, Hanoi, 100000, Vietnam; 6Vietnam National Cancer Hospital, Hanoi, 100000, Vietnam; 7Hanoi Medical University Hospital, Hanoi, 100000, Vietnam; 8Department of Biology and Medical Genetic, Hanoi Medical University, Hanoi, 100000, Vietnam; 9Genetic Counseling Center, Hanoi Medical University Hospital, Hanoi, 100000, Vietnam; 10Hanoi Medical University – Thanh Hoa Campus, Thanh Hoa, 440000, VietnamCorrespondence: Trang Thi Nguyen, Genetic Counseling Center, Hanoi Medical University Hospital, Hanoi, 100000, Vietnam, Email trangnguyen@hmu.edu.vnBackground: Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) improve the quality of life in individuals with EGFR mutation-positive non-small cell lung cancer (NSCLC). This study evaluates the treatment outcomes of EGFR-mutant NSCLC patients with concurrent gene alterations, aiming to determine their predictive significance concerning responses to EGFR-TKI therapy.Materials and Methods: We conducted a retrospective cohort study using next-generation sequencing (NGS) data from January 2019 to June 2023. …”
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Zi Shen Wan Fang repaired blood–brain barrier integrity in diabetic cognitive impairment mice via preventing cerebrovascular cells senescence by Qingsheng Yin, Genhui Yang, Runtao Su, Jie Bu, Ying Li, Han Zhang, Yanjun Zhang, Pengwei Zhuang

“…Cognitive function was evaluated using MWM, blood–brain-barrier (BBB) integrity was tested using immunostaining and western blot, the underlying mechanisms were explored using single-cell RNA sequencing (scRNA-seq), validation experiments were performed with immunofluorescence analysis, and the potential active ingredients of ZSWF against cerebrovascular senescence were predicted using molecular docking. …”
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KLF5 enhances CXCL12 transcription in adipose-derived stem cells to promote endothelial progenitor cells neovascularization and accelerate diabetic wound healing by Yunjia Xie, Xuejun Ni, Xiaofen Wan, Nating Xu, Lu Chen, Chensheng Lin, Xi Zheng, Beichen Cai, Qian Lin, Ruonan Ke, Tao Huang, Xuefeng Hu, Biao Wang, Xiuying Shan

“…Mouse adipose-derived stem cells (ADSCs) from C57BL/6 mice were evaluated in vitro for their effects on EPCs under HG using EdU, Transwell, and tube formation assays. …”
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INVESTIGATION OF THE GENETIC ALTERATIONS IN CDKN2A/B AND IL7R IN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA by RMB Lemos, ES Costa, T Ferraz, MT Schramm, MM Lins, MCN Fagundes, ALT Maciel, M Emerenciano

“…The SALSA MLPA P335-C1 kit (MRC Holland), multiplex ligation-dependent probe amplification (MLPA) technique, was used to evaluate the presence of copy number alterations (CNA) in genes related to lymphopoiesis and commonly altered in ALLs, such as CDKN2A/B. …”
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PPIH Expression Correlates with Tumor Aggressiveness and Immune Dysregulation in Hepatocellular Carcinoma by Bei J, Sun Z, Fu R, Huang X, Huang J, Luo Y, Li Y, Chen Y, Wei Z

“…This study investigates the role of Peptidyl-prolyl isomerase H (PPIH) in HCC by analyzing its expression, prognosis, methylation levels, and relationship with immune cell infiltration.Methods: We utilized bulk sequencing and clinical data from UCSC Xena and the GTEx database for preprocessing and subsequent differential expression analysis of PPIH in tumor and adjacent normal tissues, evaluating prognostic parameters like overall survival and disease-free interval between low and high PPIH expression groups. …”
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Baseline serum EV-miR-1-3p as a protective factor and biomarker for hepatocellular carcinoma development after HCV eradication by Wei Teng, Wei-Ting Ku, Po-Ting Lin, Guan-Ting Chen, LiChieh Julie Chu, Hsuan Liu, Yung-Chang Lin, Chun-Yen Lin

“…EV-miRNA profiles were assessed by small RNA sequencing and validated using a miRNA enzyme immunoassay (miREIA). …”
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Mechanism study of KCMF1 promoting proliferation and NF-κB signaling transduction in colorectal cancer cells by WU Zhibai, XU Guiqin, ZHANG Li, YANG Zhaojuan, LIU Yun, JIAO Kun, CHEN Zehong, XU Chen, ZUO You, ZHENG Ningqian, YE Zhiqian, LIU Yongzhong

“…Changes in the transcriptional profile in HCT116 cells upon KCMF1 knockdown were identified by RNA sequencing (RNA-Seq), and the affected signaling pathways were evaluated by bioinformatics analysis. …”
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético by Roca William M., Tohme Joseph, López F. Yamel, Ramírez Hernando, Mayer Jorge E.

“…Around 80% of the unique clones were single copy, and 20% were low copy sequences as expected from a cDNA library. Three pairs of parental bean lines were chosen for their agronomical traits, and evaluated for polymorphism, which was highest as revealed by digestion with EcoRV (77%), followed by DraI (73%), EcoRI (63%) and HindIIl (60%). …”
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Assessment of hemodynamic disturbances and impaired ventricular filling in asymptomatic fontan patients: A 4D flow CMR study by Li-Wei Hu, Xiaodan Zhao, Shuang Leng, RongZhen Ouyang, Qian Wang, Ai-Min Sun, Yi-Man Liu, Wei Dong, Liang Zhong, Yu-Min Zhong

“…The study utilized cine images to analyze cardiac function and inter-ventricular mechanical dyssynchrony. Echocardiography evaluated functional ventricular diastolic dysfunction. …”
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A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner by Xin-Yu Ding, Hua-Ying Zhang, Jia-Hao Chen, Meng-Jie Yang, Zhi-Xiong Huang, Yi-Hong Lei, Qin-Kun Sun, Jian-Bin Bai, Dian-Chao Lin, Jian-Fa Lan, Lu-Lu Ren, Zheng-Yi Chen, Wei-Dong Zhou, Qiong-Hua Chen

“…Functional assays (CCK-8, EdU, Transwell, and wound healing) were used to evaluate phenotypic changes. m6A immunoprecipitation sequencing (MeRIP-seq) identified downstream targets. …”
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Acasunlimab, an Fc-inert PD-L1×4-1BB bispecific antibody, combined with PD-1 blockade potentiates antitumor immunity via complementary immune modulatory effects by Maria Jure-Kunkel, Christina Yu, Nora Pencheva, Mark Fereshteh, Alexander Muik, Aras Toker, Özlem Türeci, Tahamtan Ahmadi, Ugur Sahin, Brandon W Higgs, Jordan M Blum, Saskia M Burm, Vanessa M Spires, Angelica Sette, Sina Fellermeier-Kopf, Andrea Imle, Michela Capello, Kristin Strumane, Theo Plantinga, Craig J Thalhauser, Kristina B Nürmberger, Patricia Garrido Castro, Carol Costa Sa, Bruna de Andrade Pereira

“…The acasunlimab and pembrolizumab combination is being evaluated in Phase 2 (NCT05117242) and pivotal Phase 3 (NCT06635824) trials in patients with metastatic non-small cell lung cancer after checkpoint inhibitor therapy failure.…”
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Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches by Rozi, Wiwiek Tyasningsih, Jola Rahmahani, Eduardus Bimo Aksono, Muchammad Yunus, Mohammad Anam Al-Arif, Suryo Kuncorojati, Rahayu Kusdarwati, Putri Desi Wulan Sari, Mohammad Noor Azmai Amal, Annas Salleh, Nadeem Khanand, Suwarno

“…The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. …”
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<i>Achnatherum inebrians</i> Bacterial Communities Associated with <i>Epichloë gansuensis</i> Endophyte Infection Under Low-Concentration Urea Treatment: Links to Plant Growth and... by Yuanyuan Jin, Zhenjiang Chen, Kamran Malik, Chunjie Li

“…In addition to the in vitro test, we evaluated growth performance for <i>Epichloë</i> endophyte–infected plants (E+) and <i>Epichloë</i> endophyte–free plants (E−) in a pot trial under 0.01 mol/L urea treatment. …”
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PD-1 inhibition disrupts collagen homeostasis and aggravates cardiac dysfunction through endothelial-fibroblast crosstalk and EndMT by Zejin Zhang, Zejin Zhang, Zhenzhen Yan, Zhenzhen Yan, Tao Yuan, Tao Yuan, Xiaona Zhao, Xiaona Zhao, Minghui Wang, Minghui Wang, Guoqing Liu, Guoqing Liu, Lijun Gan, Wei Qin, Wei Qin, Wei Qin

“…Single-nucleus RNA sequencing was performed to examine the effects of PD-1 inhibition on gene expression in cardiac fibroblasts (CFs) and endothelial cells (ECs). …”
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Identification of Escherichia coli 166 isolate as an effective inhibitor of African swine fever virus replication by Jinya Zhang, Hongyu Cui, Zhenjiang Zhang, Wenqing Wang, Fengwei Jiang, Encheng Sun, Yuanmao Zhu, Fang Li, Zhigao Bu, Dongming Zhao

“…In this study, we isolated bacteria from the intestinal contents of wild boar using culture-based methods and identified them through 16S ribosomal DNA (rDNA) sequencing. These isolates underwent high-throughput screening to evaluate their immunomodulatory effects on J774-Dual cells and their ability to inhibit ASFV replication in vitro. …”
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Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti... by Arndt Borkhardt, Nan Qin, Stephen T Keir, Darell D Bigner, Allison Cole, Matthias Wölfl, Viktoria Marquardt, Johanna Theruvath, David Pauck, Daniel Picard, Lena Blümel, Mara Maue, Jasmin Bartl, Ulvi Ahmadov, Maike Langini, Frauke-Dorothee Meyer, Joselyn Cruz-Cruz, Claus M Graef, Till Milde, Olaf Witt, Anat Erdreich-Epstein, Gabriel Leprivier, Ulf Kahlert, Anja Stefanski, Kai Stühler, Julia Hauer, Thomas Beez, Christiane B Knobbe-Thomsen, Ute Fischer, Jörg Felsberg, Finn K Hansen, Rajeev Vibhakar, Sujatha Venkatraman, Samuel H Cheshier, Guido Reifenberger, Thomas Kurz, Marc Remke, Siddhartha Mitra

“…Here, we report a cross-entity, epigenetic drug screen to evaluate therapeutic vulnerabilities in MYC amplified MB, which sensitizes them to macrophage-mediated phagocytosis by targeting the CD47-signal regulatory protein α (SIRPα) innate checkpoint pathway.Methods We performed a primary screen including 78 epigenetic inhibitors and a secondary screen including 20 histone deacetylase inhibitors (HDACi) to compare response profiles in atypical teratoid/rhabdoid tumor (AT/RT, n=11), MB (n=14), and glioblastoma (n=14). …”
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Early-stage endometrioid carcinoma with MSH6 protein deficiency: pitfalls in the diagnostic interpretation of microsatellite instability by Cheng Wang, Cheng Wang, Dongni Liang, Dongni Liang, Wei Wang, Wei Wang, Wei Kuang, Wei Kuang, Juan Zou, Juan Zou, Jing Zeng, Jing Zeng, Min Feng, Min Feng

“…PCR capillary electrophoresis (PCR-CE) was performed in all cases and further next-generation sequencing (NGS) was performed in non-MSI-high cases. …”
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Circulating tumour cells &amp; circulating tumour DNA in patients with resectable colorectal liver metastases (MIRACLE): a prospective, observational biomarker studyResearch in con... by Lissa Wullaert, Maurice P.H.M. Jansen, Jaco Kraan, Yannick M. Meyer, Kelly Voigt, Stavros Makrodimitris, Vanja de Weerd, Corine M. Beaufort, Mai Van, Maarten Vermaas, Eric J.T. Belt, Paul D. Gobardhan, Stefan Sleijfer, Henk M.W. Verheul, John W.M. Martens, Dirk J. Grünhagen, Saskia M. Wilting, Cornelis Verhoef, Ninos Ayez, Jan H. Wijsman, Arjen M. Rijken, Pascal Doornebosch, Joost van der Hoeven, Boris Galjart, Diederik J. Höppener, Peter M.H. Nierop, Eric P. van der Stok, Jean Helmijr, Lindsay Angus, Pauline A.J. Mendelaar, Manouk K. Bos, Elisabeth M. Jongbloed, Khrystany T. Isebia, Noortje Verschoor, Ronald van Marion, Peggy Atmodimedjo, Hendrikus J. Dubbink

“…Findings: ctDNA was detected in 117/187 patients (63%) at baseline, and 28/104 evaluable patients (27%) still had detectable ctDNA postoperatively. …”
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