Targeting PRMT1-mediated methylation of TAF15 to protect against myocardial infarction by inhibiting ferroptosis via the GPX4/NRF2 pathway by Guanshen Huang, Liwei He, Bishan Liang, Mingjian Gao, Jianming Huang, Hao Xia, Xinyu Li, Hai Li, Yunjun Ruan

“…TAF15 expression was evaluated in clinical AMI patient blood samples, ischemia/reperfusion (I/R)-treated HL-1 cardiomyocytes, and myocardial tissues from the AMI mouse model using qRT-PCR and Western blot analyses. …”
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Correlative Analysis of Tumor-Informed Circulating Tumor DNA (ctDNA) and the Survival Outcomes of Patients with Pancreatic Adenocarcinoma by Yuqi Zhang, Abdullah Esmail, Hala Hassanain, Vikramjit Dhillon, Waseem Abdelrahim, Ebtesam Al-Najjar, Bayan Khasawneh, Maen Abdelrahim

“…Clinical outcomes, including progression-free survival (PFS) and OS, were evaluated in relation to ctDNA status. <b>Results:</b> Among 39 patients, 153 plasma samples were analyzed, with 17 patients testing positive for ctDNA. …”
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Exogenous Nucleotides Supplementation Attenuates Age‐Related Sarcopenia by Xin Wu, Rui Liu, Na Zhu, Xiujuan Wang, Chan Wei, Xiaoyang An, Meihong Xu, Yong Li

“…The immunofluorescence staining was used to evaluate the cross‐sectional area (CSA) or type of muscle fibers and the diameter of myotubes. …”
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Gut dysbiosis is associated with increased blood–brain barrier permeability and cognitive impairment in elderlies with coronary heart disease by Lichao Di, Peiying Huang, Yeju He, Na Sun, Liwei Chi, Lining Huang, Lining Huang, Lining Huang

“…Fecal samples underwent 16S rRNA gene sequencing (V3-V4 region) to evaluate gut microbiota diversity and composition. …”
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Antagonistic impact of Lactobacilli on Escherichia coli in the gut of Labeo rohita fingerlings fed with bakery wastes by Abdul Rehman, Fayyaz Rasool, Shakeela Parveen, Aqsa Tahir, Mati Ullah, Syed Shafat Hussain, Nimra Khalid, Harun Arslan

“…The beneficial growth promoting lactobacilli and pathogenic Escherichia coli bacteria were isolated from the gut of Labeo rohita fingerlings and evaluated the antagonistic impact of lactobacilli on E. coli. …”
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Xingxiao Pill Suppressed the Progression of Non-Small Cell Lung Cancer by Targeting SREBP1/FASN&amp;ndash;Induced Fatty Acid Biosynthesis via PI3K/AKT/mTOR Signaling Pathway by Zhou X, Hu X, Zhang Z, Lin S, Lin X, Zhou T, Bai Y, Hu K

“…Bioinformatics analysis and transcriptome sequencing identified XXP’s potential targets and mechanisms. …”
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Ruminal Yeast Strain with Probiotic Potential: Isolation and Characterization and Its Effect on Rumen Fermentation In Vitro by Pin Song, Xiaoran Yang, Manman Hou, Yue Chen, Liping Liu, Yuyan Feng, Yingdong Ni

“…A number of tests were conducted to assess their anaerobic traits, growth rate, acid tolerance, and lactate utilization ability, and a second screening in fresh ruminal fluid to evaluate in vitro pH and acid accumulation was conducted. …”
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Gemcitabine resistance by CITED4 upregulation via the regulation of BIRC2 expression in pancreatic cancer by Eun-Jeong Jeong, Yuna Roh, Eunsun Jung, Jin-Seong Hwang, Taesang Son, Hyun Seung Ban, Tae-Su Han, Young-Kug Choo, Jang-Seong Kim

“…Transcriptomic data from control or GEM-resistant tumor-derived cells were analyzed. GEM resistance was evaluated using cell viability, clonogenicity, and apoptosis assays. …”
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CO40 | The co-inheritance of two ITGB3 variants exerting additive detrimental effects on platelets leads to variant Glanzmann thrombasthenia

“…Methods: Patient platelet function was assessed and DNA sequenced by HTS-gene panel. Expression vectors were generated, and αII-bearing CHO cells were transfected with p.Asn3331Ser, p.Leu20Arg, or both β3 variants. …”
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LncRNA PTS-1 Protects Against Osteoarthritis Through the miR-8085/E2F2 Axis by Ma C, Chen Q, Wei YF, Chen SW, Liu H, Xin F, Ren YX

“…This study focuses on lncRNA PTS-1, a novel lncRNA, to explore its function and regulatory mechanisms in OA progression.Methods: The expression profile of lncRNAs was assessed using RNA sequencing and qRT-PCR. The expression of lnc-PTS-1 was further validated by qRT-PCR in degenerated cartilage tissues, degenerative primary chondrocytes, and IL-1β-treated C28/I2 cells. …”
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Ultra-Wideband Analog Radio-over-Fiber Communication System Employing Pulse-Position Modulation by Sandis Migla, Kristaps Rubuls, Nikolajs Tihomorskis, Toms Salgals, Oskars Ozolins, Vjaceslavs Bobrovs, Sandis Spolitis, Arturs Aboltins

“…System performance was evaluated by transmitting pseudorandom binary sequences (PRBSs) and measuring the bit error ratio (BER) across a 5-m wireless link between two 20 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">d</mi><mi mathvariant="normal">B</mi><mi mathvariant="normal">i</mi></mrow></semantics></math></inline-formula> gain horn (Ka-band) antennas, with and without a 20 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="normal">k</mi><mi mathvariant="normal">m</mi></mrow></semantics></math></inline-formula> single-mode optical fiber (SMF) link in transmitter side and ECC at the receiver side. …”
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Genetic surveillance shows spread of ACT resistance during period of malaria decline in Vietnam (2018-2020) by Johanna Helena Kattenberg, Mathijs Mutsaers, Van Hong Nguyen, Thi Hong Ngoc Nguyen, Arlette Umugwaneza, Maria Lara-Escandell, Xuan Xa Nguyen, Thi Huong Binh Nguyen, Anna Rosanas-Urgell

“…Variants in 14 putative resistance genes, including P. falciparum Kelch 13 (PfK13) and P. falciparum chloroquine resistance transporter (Pfcrt), were analyzed and within-country parasite diversity was evaluated. Other targets included KEL1-lineage markers and diagnostic markers of Pfhrp2/3.ResultsA concerning level of DHA-PPQ resistance was detected. …”
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Toll-like receptor 8 agonists improve NK-cell function primarily targeting CD56brightCD16− subset by Enrico Maggi, Giovanni Scambia, Anna Fagotti, Lorenzo Moretta, Andrea Pelosi, Irene Veneziani, Valentina D'Oria, Claudia Alicata, Nadine Landolina, Biancamaria Ricci

“…Natural killer (NK) cells have been described to express different endosomal TLRs triggered by RNA and DNA sequences derived from both viruses and bacteria. This study was addressed to establish which endosomal TLR could directly mediate NK activation and function after proper stimuli. …”
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IGF2BP3 enhances ferroptosis resistance in colon cancer by stabilizing SLC7A11 and is regulated by miR-98-5p by Yaya Sun, Qingwei Liu, Junjie Wu, Guangqing Jiang, Hongzhou Shi, Yihong Zhang, Yanxuan Ling, Weimin Sun, Xin Shi, Congxing Liu

“…Ferroptosis sensitivity was evaluated via CCK-8 assays, MDA/ROS quantification, and rescue experiments with ferrostatin-1. …”
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An Efficient and Cost-Effective Vehicle Detection and Tracking System for Collision Avoidance in Foggy Weather by Naeem Raza, Muhammad Asif Habib, H. M. Sajid Imran, Abdul Qayum, Sajida Perveen, Sohail Jabbar, Shahzad Sarwar, Mudassar Ahmad

“…The optimized deep-SORT outperforms pre-processing, inference, NMS post-processing, and update time, achieving higher FPS on foggy and the BDD100K dataset video sequences compared to the baseline deep-SORT and strong-SORT vehicle tracking algorithms. …”
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Alteration in the expression of MGMT and RUNX3 due to non-CpG promoter methylation and their correlation with different risk factors in esophageal cancer patients by Snigdha Saikia, Asad Ur Rehman, Prajjalendra Barooah, Preeti Sarmah, Mallika Bhattacharyya, Muktanjalee Deka, Manab Deka, Bhabadev Goswami, Syed Akhtar Husain, Subhash Medhi

“…The promoter methylation was evaluated for O 6 -methylguanine-DNA methyltransferase and runt-related transcription factor 3 in CpG, CHH, and CHG context (where H is A, T, or C) by next-generation sequencing. …”
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Activation of TLR4/CCL2 in Intact Neurons Drives Radicular Injury-Induced Global Nerve Trunk Hypersensitivity in Radiculopathy Preclinical Models by Tong SH, Zhou J, Ye F, Ding P, Mei JL, Liao P, Lu YF, Zong Y, Gao CA, Zhang SY, Gao JJ, Liu DL, Huang YG

“…Nociceptive behaviors were evaluated by von Frey testing and gait analysis. Immunofluorescence stainings were employed to analyze protein expression levels. …”
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Characterization and natural history of patients with LMNA‐related dilated cardiomyopathy in the phase 3 REALM‐DCM trial by Pablo Garcia‐Pavia, Neal K. Lakdawala, Gianfranco Sinagra, Tomas Ripoll‐Vera, Kia Afshar, Silvia G. Priori, James S. Ware, Anjali Owens, Huihua Li, Franca S. Angeli, Perry Elliott, Calum A. MacRae, Daniel P. Judge

“…The phase 3 REALM‐DCM trial evaluated a potential disease‐modifying therapy for LMNA‐related DCM but was terminated due to futility without safety concern. …”
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S100A4 enhances protumor macrophage polarization by control of PPAR-γ-dependent induction of fatty acid oxidation by Xu Chen, Yanan Li, Zhaoqing Wang, Shuangqing Liu, Huilei Zhang, Yana Zhang, Yangyang Bian, Yanqiong Zeng, Xiaohan Yao, Jiajia Wan, Jianru Li, Zhihai Qin

“…In vitro induction of macrophage M2 polarization was conducted by interleukin 4 (IL-4) stimulation. RNA-sequencing, real-time quantitative PCR, flow cytometry, western blotting, immunofluorescence staining and mass spectrometry were used to determine macrophage phenotype. …”
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SSL5-AnxA5 Fusion Protein Constructed Based on Human Atherosclerotic Plaque scRNA-Seq Data Preventing the Binding of Apoptotic Endothelial Cells, Platelets, and Inflammatory Cells by Yifei Zhao, Xingyu He, Teng Hu, Tianli Xia, Fangyang Huang, Changming Li, Yiming Li, Fei Chen, Mao Chen, Jun Ma, Yong Peng

“…Through single-cell sequencing analysis (scRNA-seq) of plaques from SAP and ACS patients, we identified significant changes in the annexin V and P-selectin glycoprotein ligand 1 pathways. …”
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