Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses by Dequan Yang, Li Ma, Zhongping Yang, Xianchao Yang, Jian Wang, Houbin Ju, Chunguang Lu, Yonggang Weng, Heping Zhao, Haixiao Shen, Xin Li, Feifei Ge, Xiaoxu Wang, Xiujuan Wu, Meng Xiang, Guidan Feng, Congsheng Tang, Shixin Huang, Hongjin Zhao

“…The sensitivity, specificity, and reproducibility ability were evaluated for the mRT-qPCR. Further, 80 bovine fecal samples were subjected to the mRT-qPCR, and the results were verified using conventional reverse-transcription PCR (RT-PCR) or PCR methods and sequencing methods.ResultsThis novel method demonstrated high sensitivity and specificity,achieving a detection limit of 24 copies/mL for each pathogen. …”
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Lysin (K)-specific demethylase 1 inhibition enhances proteasome inhibitor response and overcomes drug resistance in multiple myeloma by Cecilia Bandini, Elisabetta Mereu, Tina Paradzik, Maria Labrador, Monica Maccagno, Michela Cumerlato, Federico Oreglia, Lorenzo Prever, Veronica Manicardi, Elisa Taiana, Domenica Ronchetti, Mattia D’Agostino, Francesca Gay, Alessandra Larocca, Lenka Besse, Giorgio Roberto Merlo, Emilio Hirsch, Alessia Ciarrocchi, Giorgio Inghirami, Antonino Neri, Roberto Piva

“…We confirmed the combo treatment’ synergistic effects ex vivo in primary CD138+ cells from MM patients, and in different MM xenograft models. We exploited RNA-sequencing and Reverse-Phase Protein Arrays (RPPA) to investigate the molecular mechanisms of the synergy. …”
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Resource utilization of decarbonized coal gasification slag in soil quality improvement: New insights into microbial community composition and environmental risk assessment by Longfei Kang, Qiang Li, Kenneth Dumack, Kaiyu Zhang, Yulin Xiang, Jiangdong Bian, Feng Ai

“…Here, a field trial was conducted to evaluate the feasibility of soil amendment using DCGS in a sandy soil. …”
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NOS3 rs3918188C>A is associated with susceptibility to resistant hypertension while CES1 genetic variation was not associated with resistant hypertension among South Africans by Jonathan N. Katsukunya, Jonathan N. Katsukunya, Revina Naicker, Revina Naicker, Nyarai D. Soko, Nyarai D. Soko, Nyarai D. Soko, Dirk Blom, Dirk Blom, Phumla Sinxadi, Phumla Sinxadi, Emile R. Chimusa, Brian Rayner, Brian Rayner, Erika Jones, Erika Jones, Collet Dandara, Collet Dandara

“…IntroductionGenetic variation in genes coding for enzymes metabolising antihypertensive drugs, may affect the efficacy of angiotensin converting enzyme (ACE) inhibitors such as enalapril, potentially leading to resistant hypertension (RHTN). We set out to evaluate the contribution of genetic variation in CES1 and NOS3 genes on susceptibility to RHTN, as well as estimate the frequencies of CES1 copy number variation (CNV) in African and Mixed Ancestry (MA) populations of South Africa.MethodsUsing a retrospective age, sex and ethnicity matched case-control study design, 379 participants with hypertension belonging to the African and MA ethnic groups were recruited. …”
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Troxerutin suppresses the stemness of osteosarcoma via the CD155/SRC/β-catenin signaling axis by Junkai Chen, Hongbo Li, Qinglin Jin, Xiaoguang Li, Yiwen Zhang, Jingnan Shen, Gang Huang, Junqiang Yin, Changye Zou, Xinyu Li, Xin He, Xianbiao Xie, Tiao Lin

“…The sphere formation experiment was used to evaluate the stemness of tumor cells. Additionally, in vivo animal models were utilized to assess the functional role of CD155 in a biological context. …”
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Connexin 43 suppression enhances contractile force in human iPSC-derived cardiac tissues by Takuma Takada, Takuma Takada, Katsuhisa Matsuura, Katsuhisa Matsuura, Katsuhisa Matsuura, Tatsuro Iida, Tatsuro Iida, Toshiharu Koike, Toshiharu Koike, Hidekazu Sekine, Yuhei Higashi, Yuhei Higashi, Tsukasa Hara, Daisuke Sasaki, Kyohei Fujita, Yuto Hinata, Yuto Hinata, Junichi Yamaguchi, Tatsuya Shimizu

“…This study aimed to evaluate whether regulating GJA1, the gene encoding Cx43, could enhance contractility and synchrony in these tissues. …”
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Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis by Shimaa K. Ali, F.M Mohamed, Ahmed H. El-Ghorab, Eman A. Hamed, Moustafa A. Aboel-Ainin, Mohamed A. Abdelgawad, Khaled El‐Adl, Hussein S. Mohamed

“…The antibacterial and antitumor activities of the polymer were evaluated through biological assays. The antibacterial activity was tested against gram-positive microorganisms (such as Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus) and gram-negative organisms (such as Shigella sonnei and Escherichia coli). …”
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Therapeutic depletion of CCR8+ tumor-infiltrating regulatory T cells elicits antitumor immunity and synergizes with anti-PD-1 therapy by James Dooley, Bart Neyns, Elizabeth Allen, Frank Tacke, Louis Boon, Damya Laoui, Julia Katharina Schwarze, Helena Van Damme, Bruno Dombrecht, Máté Kiss, Heleen Roose, Eva Van Overmeire, Daliya Kancheva, Liesbet Martens, Aleksandar Murgaski, Pauline Madeleine Rachel Bardet, Gillian Blancke, Maude Jans, Evangelia Bolli, Maria Solange Martins, Yvon Elkrim, Kiavash Movahedi, Niels Vandamme, Sebahat Ocak, Isabelle Scheyltjens, Lars Vereecke, Frank Aboubakar Nana, Pascal Merchiers, Jo Agnes Van Ginderachter

“…The therapeutic use of these Nb-Fc fusion proteins was evaluated, either as monotherapy or as combination therapy with anti-programmed cell death protein-1 (anti-PD-1), in both the LLC-OVA and MC38 mouse models.Results We were able to discern two ti-Treg populations, one of which is characterized by the unique expression of Ccr8 in conjunction with Treg activation markers. …”
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Does the alternating timing of rTMS combined with soft-hand rehabilitation robot affect the recovery of hand function in patients after stroke? A study protocol for a multicentre r... by Ying Shen, Jiang Xu, Ting Zhou, Xinyue Wang, Hongxing Wang, Wenjun Dai, Chuan Guo, Xuejing Li, Xiaobing Chen, Kai Leng Catherine Chan, Fei Lu, Qinglun Su

“…The efficacy of this combination may depend on the sequencing of the interventions. However, few studies have explored the possibility of interleaving or alternating between the two treatment modalities within a single session or over a shorter time frame. …”
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Brown adipose tissue-derived extracellular vesicles regulate hepatocyte mitochondrial activity to alleviate high-fat diet-induced jawbone osteoporosis in mice by Kai Zhang, Kai Zhang, Sha Zhang, Sha Zhang, Sha Zhang, Guorong Deng, Guangxiang He, Guangxiang He, Yuan Yuan, Yu Fu, Yihan Liu, Zhen Gong, Liang Kong, Chenxi Zheng

“…The additional benefits of BEV on the osteogenic microenvironment were evaluated via AML12/MC3T3-E1 indirect co-culture under high-lipid conditions. …”
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Genetic landscape of acute myeloid leukemias with leukocytosis by K. A. Pekhova, Yu. V. Sidorova, N. A. Severina, O. A. Glinshchikova, I. S. Fevraleva, B. V. Biderman, Yu. A. Chabaeva, S. M. Kulikov, I. A. Luk’yanova, A. I. Kashlakova, T. N. Obukhova, V. N. Dvirnyk, A. B. Sudarikov

“…Tumor cell proliferation in acute myeloid leukemia (AML) may manifest with high leukocyte counts. In our work, we evaluate the association of high leukocyte counts with individual mutations, as well as their total contribution to the development of leukocytosis in AML. …”
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The Society for Cardiovascular Magnetic Resonance Registry at 150,000 by Matthew S. Tong, Jeremy A. Slivnick, Behzad Sharif, Han W. Kim, Alistair A. Young, Lilia M. Sierra-Galan, Kanae Mukai, Afshin Farzaneh-Far, Sadeer Al-Kindi, Angel T. Chan, George Dibu, Michael D. Elliott, Vanessa M. Ferreira, John Grizzard, Sebastian Kelle, Simon Lee, Maan Malahfji, Steffen E. Petersen, Venkateshwar Polsani, Olga H. Toro-Salazar, Kamran A. Shaikh, Chetan Shenoy, Monvadi B. Srichai, Jadranka Stojanovska, Qian Tao, Janet Wei, Jonathan W. Weinsaft, W. Benjamin Wince, Priya D. Chudgar, Matthew Judd, Robert M. Judd, Dipan J. Shah, Orlando P. Simonetti

“…ABSTRACT: Background: Cardiovascular magnetic resonance (CMR) is increasingly utilized to evaluate expanding cardiovascular conditions. The Society for Cardiovascular Magnetic Resonance (SCMR) Registry is a central repository for real-world clinical data to support cardiovascular research, including those relating to outcomes, quality improvement, and machine learning. …”
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Dose–response study of a fenugreek-based antibiotic alternative in Bábolna Tetra-SL chicks (1–42 days old) with mixed bacterial infections by Ádám Kerek, Ádám Kerek, Ábel Szabó, Péter Ferenc Dobra, Krisztina Bárdos, Krisztina Bárdos, Bettina Paszerbovics, Zsófia Bata, Viviána Molnár-Nagy, Ákos Jerzsele, Ákos Jerzsele, László Ózsvári, László Ózsvári

“…Future research will involve large-scale industrial trials and next-generation sequencing to evaluate the additive’s impact on gut microbiota composition.…”
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Beyond genomics: artificial intelligence-powered diagnostics for indeterminate thyroid nodules—a systematic review and meta-analysis by Karishma Jassal, Karishma Jassal, Melissa Edwards, Afsaneh Koohestani, Afsaneh Koohestani, Wendy Brown, Jonathan W. Serpell, Jonathan W. Serpell, James C. Lee, James C. Lee

“…We conducted this review to analyse the current evidence of AI in ITN diagnosis without the use of GSC.MethodsEnglish language articles evaluating the diagnostic accuracy of AI for ITNs were identified. …”
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CircABCA1 promotes ccRCC by reprogramming cholesterol metabolism and facilitating M2 macrophage polarization through IGF2BP3-mediated stabilization of SCARB1 mRNA by Hao Ning, Yan Jiang, Binbin Li, Junwu Ren, Cong Wang, Ling Wei, Linfei Li, Ai Ran, Zuozhang Li, Jiao Li, Wei Li, Yongquan Wang, Bin Xiao

“…Untargeted metabolomics, RNA- sequencing, and MS2 RNA-pulldown were conducted to identify targets. …”
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Lycium barbarum and Lactobacillus acidophilus synergistically protect against anti-tuberculosis drug-induced male reproductive injury via gut microbiota-independent pathways in mic... by Xiaoyong Song, Wei Guan, Wei Guan, Zhimin Du, Yi Gong, Dan Wang, Yajun Xiong, Yuting Gao, Xinli Shi

“…Afterwards, sperm motility, count, and serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) levels were evaluated. Gut contents were collected for 16S rRNA sequencing and real-time PCR, and testicular tissues were subjected to pathological and transcriptomic analyses.ResultsWolfberry improved sperm quality in mice with reproductive injury induced by anti-TB drugs. …”
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Synergistic Induction of Immunogenic Cell Death by Biomineralized Manganese and Bisphosphonates Enhances Anti-PD-L1 Therapy in Triple-Negative Breast Cancer by Zhang X, Liu N, Wei M, Yang C, Lin Y, Zeng Y, Li Y, Zhou L, Li T, Zheng Q, Yu H, Zhang J, Gu Y, Xia N, Li S

“…In 4T1 tumor models, we evaluated the synergistic anti-tumor effect of Mn²+ and BPs with anti-PD-L1 antibodies.Results: By leveraging the doping capacity of hydroxyapatite (HA) for Mn²+ and its high affinity for BPs, we developed MnHARis particles—a biocompatible slow-release system of biomineralized Mn²+ and risedronate (Ris). …”
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ZO-1 boosts the in vitro self-renewal of pre-haematopoietic stem cells from OCT4-reprogrammed human hair follicle mesenchymal stem cells through cytoskeleton remodeling by Yingchun Ruan, Xingang Huang, Pengpeng Sun, Xiaozhen Yu, Xiaohua Tan, Yaolin Song, Hua Chen, Zhijing Liu

“…Proliferative capacity was evaluated through CCK-8, colony formation assays, Ki67 index, and cell cycle analysis. …”
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Yeast protein as a fishmeal substitute: impacts on reproductive performance, immune responses, and gut microbiota in two sow hybrids by Pan Zhou, Qi Liu, Yang Zhao, Yachao Wu, Jianbo Shen, Tao Duan, Long Che, Yong Zhang, Honglin Yan

“…This study aimed to investigate the effects of yeast protein, an emerging sustainable protein source, on reproductive performance, immune responses, and gut microbiota in two-way crossbred sows (Landrace × Yorkshire, LY) and LLY sows.MethodsThe experiment employed a 2×2 factorial design evaluating two fixed factors: sow hybrid (LY vs LLY) and yeast protein supplementation (0% vs 2.6%). …”
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Ceftazidime-Induced Overexpression of MexJK-OprM Confers Resistance to Ceftolozane/Tazobactam in an Isolate of Pseudomonas aeruginosa by Sato T, Tamura M, Kaimori K, Okumura F, Ikejima S, Nakai H, Kawamura M, Fujimura S

“…Carbapenemase genes were detected via multiplex PCR. Sequencing and expression analyses of ampC, ftsI, oprD, and mex family genes were conducted. …”
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